Anti-inflammatory effects of the prostacyclin analogue iloprost in an in vitro model of inflamed human dental pulp cells
Rahman Wahyudi DDS
Faculty of Dentistry, Graduate Program in Oral Biology, Chulalongkorn University, Bangkok, Thailand
Search for more papers by this authorSonntana Seang DDS, PhD
Faculty of Dentistry, Graduate Program in Oral Biology, Chulalongkorn University, Bangkok, Thailand
Department of Oral and Maxillofacial Surgery and Dentistry, Khmer-Soviet Friendship Hospital, Phnom Penh, Cambodia
Search for more papers by this authorVincent Everts PhD
Faculty of Dentistry, University of Amsterdam and Vrije University Amsterdam, Amsterdam, The Netherlands
Department of Anatomy, Faculty of Dentistry, Chulalongkorn University, Bangkok, Thailand
Search for more papers by this authorThanaphum Osathanon DDS, PhD
Department of Anatomy, Faculty of Dentistry, Chulalongkorn University, Bangkok, Thailand
Dental Stem Cell Biology Research Unit, Faculty of Dentistry, Chulalongkorn University, Bangkok, Thailand
Search for more papers by this authorCorresponding Author
Chalida Nakalekha Limjeerajarus DDS, PhD
Department of Physiology, Faculty of Dentistry, Chulalongkorn University, Bangkok, Thailand
Center of Excellence in Genomics and Precision Dentistry, Faculty of Dentistry, Chulalongkorn University, Bangkok, Thailand
Correspondence
Chalida Nakalekha Limjeerajarus, Department of Physiology, Faculty of Dentistry, Chulalongkorn University, Bangkok, Thailand.
Email: [email protected]
Search for more papers by this authorRahman Wahyudi DDS
Faculty of Dentistry, Graduate Program in Oral Biology, Chulalongkorn University, Bangkok, Thailand
Search for more papers by this authorSonntana Seang DDS, PhD
Faculty of Dentistry, Graduate Program in Oral Biology, Chulalongkorn University, Bangkok, Thailand
Department of Oral and Maxillofacial Surgery and Dentistry, Khmer-Soviet Friendship Hospital, Phnom Penh, Cambodia
Search for more papers by this authorVincent Everts PhD
Faculty of Dentistry, University of Amsterdam and Vrije University Amsterdam, Amsterdam, The Netherlands
Department of Anatomy, Faculty of Dentistry, Chulalongkorn University, Bangkok, Thailand
Search for more papers by this authorThanaphum Osathanon DDS, PhD
Department of Anatomy, Faculty of Dentistry, Chulalongkorn University, Bangkok, Thailand
Dental Stem Cell Biology Research Unit, Faculty of Dentistry, Chulalongkorn University, Bangkok, Thailand
Search for more papers by this authorCorresponding Author
Chalida Nakalekha Limjeerajarus DDS, PhD
Department of Physiology, Faculty of Dentistry, Chulalongkorn University, Bangkok, Thailand
Center of Excellence in Genomics and Precision Dentistry, Faculty of Dentistry, Chulalongkorn University, Bangkok, Thailand
Correspondence
Chalida Nakalekha Limjeerajarus, Department of Physiology, Faculty of Dentistry, Chulalongkorn University, Bangkok, Thailand.
Email: [email protected]
Search for more papers by this authorAbstract
Iloprost's anti-inflammatory effects on human dental pulp stem cells (HDPCs) are currently unknown. We hypothesized that iloprost could downregulate the expression of inflammatory-related genes and protein in an inflamed HDPC in vitro model. To induce inflammation, the HDPCs were treated with a cocktail of interleukin-1 beta, interferon-gamma, and tumour necrosis alpha, at a ratio of 1:10:100. Iloprost (10−6 M) was then added or not to the cultures. Interleukin-6 (IL-6) and interleukin-12 (IL-12) mRNA expression were assessed by real-time polymerase chain reaction. IL-6 protein expression was assessed by enzyme-linked immunosorbent assay. The results were analysed using one-way ANOVA or the Kruskal–Wallis test. The cytokine cocktail induced more robust IL-6 expression than LPS treatment. Iloprost slightly, yet significantly, downregulated IL-6 and IL-12 mRNA expression. These findings suggest that iloprost might be used as a beneficial component in vital pulp therapy.
CONFLICT OF INTEREST STATEMENT
The authors deny any conflict of interest. The authors do not have any financial interest in the companies whose materials are included in this article.
Supporting Information
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