ORIGINAL ARTICLE

Influence of Huaiqihuang granule on peritoneal fibrosis induced by peritoneal dialysis: A study based on rat model

Lin Jia

Department of Nephrology, The Central Hospital of Wuhan, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, China

Key Laboratory for Molecular Diagnosis of Hubei Province, The Central Hospital of Wuhan, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, China

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Hui Liu,

Hui Liu

Department of Nephrology, The Central Hospital of Wuhan, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, China

Key Laboratory for Molecular Diagnosis of Hubei Province, The Central Hospital of Wuhan, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, China

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Yongxia Li,

Yongxia Li

Department of Nephrology, The Central Hospital of Wuhan, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, China

Key Laboratory for Molecular Diagnosis of Hubei Province, The Central Hospital of Wuhan, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, China

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Yunfang Huang,

Corresponding Author

Yunfang Huang

[email protected]

orcid.org/0009-0000-6763-9456

Department of Nephrology, The Central Hospital of Wuhan, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, China

Key Laboratory for Molecular Diagnosis of Hubei Province, The Central Hospital of Wuhan, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, China

Correspondence

Yunfang Huang, Department of Nephrology, The Central Hospital of Wuhan, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, 430014, China.

Email: [email protected]

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Lin Jia,

Lin Jia

Department of Nephrology, The Central Hospital of Wuhan, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, China

Key Laboratory for Molecular Diagnosis of Hubei Province, The Central Hospital of Wuhan, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, China

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Hui Liu,

Hui Liu

Department of Nephrology, The Central Hospital of Wuhan, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, China

Key Laboratory for Molecular Diagnosis of Hubei Province, The Central Hospital of Wuhan, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, China

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Yongxia Li,

Yongxia Li

Department of Nephrology, The Central Hospital of Wuhan, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, China

Key Laboratory for Molecular Diagnosis of Hubei Province, The Central Hospital of Wuhan, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, China

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Yunfang Huang,

Corresponding Author

Yunfang Huang

[email protected]

orcid.org/0009-0000-6763-9456

Department of Nephrology, The Central Hospital of Wuhan, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, China

Key Laboratory for Molecular Diagnosis of Hubei Province, The Central Hospital of Wuhan, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, China

Correspondence

Yunfang Huang, Department of Nephrology, The Central Hospital of Wuhan, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, 430014, China.

Email: [email protected]

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First published: 14 May 2025

https://doi.org/10.1111/1744-9987.70031

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Abstract

Introduction

To investigate the role of Huaiqihuang granule in the inhibition and immune regulation of peritoneal fibrosis in peritoneal dialysis rats.

Methods

Peritoneal fibrosis rat models were established and subsequently assigned to five groups: a normal group (Group A); a model group (Group B); a low-concentration treatment group (Group C); a medium-concentration treatment group (Group D); and a high-concentration treatment group (Group E). The normal rats served as the control group (Group A). All rats underwent intragastric administration of Huaiqihuang granules for a duration of 21 days.

Results

Peritoneal histopathological staining showed that group B rats had the highest degree of peritoneal fibrosis. In the intervention group, the degree of peritoneal fibrosis decreased with the increase of concentration of Huaiqihuang granule with a significant difference (p <0.05). Immunohistochemical analysis showed that the expression of TGF-β1, TNF-α, and α-SMA in rat peritoneal tissue was higher in group B than in group E (p< 0.05). Polymerase Chain Reaction (PCR) detection of mRNA expression of TGF-1 and fibronectin in rat peritoneal tissues: group B>group C>group D>group E>group A (p<0.05). The macrophage phagocytosis function, the levels of CD3+, CD4+, CD8+ T cells, and the CD4+/CD8+ ratio in peripheral blood were lowest in group B compared to groups C, D, and E (p < 0.05). In contrast, the levels of IL-2, IL-6, TGF-β1, and TNF-α were highest in group B (p < 0.05).

Conclusion

It is speculated that Huaiqihuang granule may be used clinically as effective drugs to improve peritoneal fibrosis and regulate immune function in peritoneal dialysis patients.

CONFLICT OF INTEREST STATEMENT

The authors declare that they have no competing interests.

Supporting Information

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Volume29, Issue4

45th Annual Meeting of Japanese Society for Apheresis

August 2025

Pages 653-661

Influence of Huaiqihuang granule on peritoneal fibrosis induced by peritoneal dialysis: A study based on rat model

Abstract

Introduction

Methods

Results

Conclusion

CONFLICT OF INTEREST STATEMENT

Supporting Information

REFERENCES

References

Information

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Influence of Huaiqihuang granule on peritoneal fibrosis induced by peritoneal dialysis: A study based on rat model

Abstract

Introduction

Methods

Results

Conclusion

CONFLICT OF INTEREST STATEMENT

Supporting Information

REFERENCES

References

Related

Information