2.1. Protein production

The nucleotide sequence encoding WbdD600 (residues 1–­600 of WbdD; accession No. JX235676) was cloned into pBAD24 as described previously (Clarke et al., 2009). The same cloning strategy was used for the WbdD556 sequence. Both constructs introduced a tobacco etch virus (TEV) cleavable N-terminal His tag (MHHHHHHENLYFQG; only the C-terminal glycine remains as the new N-terminus after TEV cleavage, a one-residue extension of the sequence of the target protein). An identical expression and purification procedure was followed for both proteins. The plasmids were transformed into Escherichia coli Rosetta cells. A single colony was selected and grown overnight in Luria broth (LB) medium containing 100 µg ml⁻¹ ampicillin. The overnight culture was used to inoculate 5 l LB medium at a ratio of 1:100. This culture was grown at 310 K with shaking at 200 rev min⁻¹. Once the culture reached an optical density (600 nm) of 0.4, the incubation temperature was lowered to 301 K. At an OD₆₀₀ of 1.0, protein expression was induced by adding L-­arabinose to a final concentration of 0.2% and the culture was incubated at 301 K for 5 h. The cells were harvested by centrifugation at 7000g for 30 min. Cell pellets containing the target proteins were resuspended in lysis buffer [20 mM bis-Tris, 250 mM NaCl, 10 mM imidazole, 5%(w/v) glycerol pH 7.0 and one Complete protease-inhibitor cocktail tablet (Roche Diagnostics) per 50 ml of extract] and the mixture was stirred at 277 K for 30 min. After treatment with a cell disrupter (207 MPa; Constant Cell Disruption Systems, Daventry, England), the lysate was clarified by centrifugation at 30 000g for 1 h at 277 K. The cell-free supernatant was loaded onto a 5 ml Ni–NTA column (GE Healthcare). Prior to loading, the resin was equilibrated in lysis buffer. The loaded resin was washed with lysis buffer and the target protein was eluted with lysis buffer containing 1 M imidazole. For final purification, the protein was passed over a Superdex 200 16/60 column (GE Healthcare) and eluted with 20 mM bis-Tris pH 7.0, 50 mM NaCl. Fractions with appropriate purity, as judged by SDS–PAGE, were pooled and concentrated to 10–20 mg ml⁻¹ for crystallization. Protein identity and integrity were confirmed by mass spectrometry. The protein was flash-cooled in liquid N₂ using thin-walled PCR tubes and stored at 193 K prior to further use.

2.2. Production of selenomethionine-labelled WbdD556

Selenomethionine-labelled WbdD556 was prepared using glucose-free SeMet medium from Molecular Dimensions. Glycerol was added at a concentration of 5% to provide a carbon source. The cells from a 100 ml overnight culture were harvested by centrifugation and resuspended in phosphate-buffered saline (PBS). The main culture was inoculated and grown for 1 h at 310 K before seleno-L-methionine (SeMet) was added (50 µg ml⁻¹). When an OD₆₀₀ of 0.5 was reached, the temperature was lowered to 301 K and protein expression was induced by adding 0.2% L-arabinose. The SeMet protein was purified in the same way as the native protein (see above).

2.3. Crystallization

Initial crystallization trials for WbdD600 and WbdD556 were performed using a Honeybee 963 robot system (Genomic Solutions) with both commercially available and self-made (Oke et al., 2010) crystallization screens. For each of the 96-well sitting-drop vapour-diffusion screens (MRC plates, Swissci), 150 nl protein solution (∼15 mg ml⁻¹ in 20 mM bis-Tris pH 7.0, 50 mM NaCl supplemented with 5 mM each of ATP, SAM and MgCl₂) was mixed with 150 nl precipitant and equilibrated against a reservoir of 75 µl precipitant. The sealed plates were then incubated at 293 K.

Initial WbdD crystals were obtained in a condition containing 51% Tacsimate pH 8.0 from the commercial Index screen (Hampton Research) and were further optimized by several rounds of stochastic optimizations in a 96-well format. The best results, as judged by crystal appearance, were obtained with a mixture of 0.26 M lithium sulfate, 0.1 M Tris–HCl pH 8.4, 1.12 M ammonium sulfate. Crystals (>100 µm) usually appeared after 1–3 d at 293 K. Prior to flash-cooling in liquid nitrogen for data collection, the crystals were cryoprotected by transferring them into either a saturated ammonium sulfate solution or mother liquor supplemented with either 30% mannose, 30% ethylene glycol or 30% glycerol.

2.4. Limited proteolysis

The proteases thermolysin, papain, trypsin and subtilisin (Sigma) were mixed with WbdD600 (1 mg ml⁻¹) in a 1:10 or a 1:100 molar ratio of protease:WbdD600. The mixtures were incubated on ice for 1 h and the reactions were stopped by adding boiling SDS–PAGE loading buffer (NuPAGE LDS sample buffer, Invitrogen). The samples were then analyzed by SDS–PAGE.

3.1. Finding a suitable crystallization construct by limited proteolysis

WbdD is a 82 kDa protein with 708 amino-acid residues and two functional domains. The N-terminus of the protein contains a methyltransferase (MTase) domain (residues 1–210) followed by a kinase domain (residues 211–459) and a C-­terminal coiled-coil domain (residues 460–708) (Fig. 1a). The full-length protein is difficult to solubilize and purify, but we found a construct comprising amino acids 1–600 of WbdD (WbdD600) that is expressed in sufficient amounts for crystallization experiments (>100 mg per litre of culture). We submitted the WbdD600 sequence to the XtalPred server (Slabinski et al., 2007) to assess the likelihood of crystallization and the protein was classified as `very difficult'. This is mainly owing to its relatively large size and the presence of multiple coiled-coil domains and the amphipathic helix (Fig. 1a). Nevertheless, the protein was purified using a combination of Ni–NTA, ion-exchange and gel-filtration chromatography (Fig. 1b, lane 2). Although more than 1000 crystallization conditions were tested at protein concentrations ranging from 5 to 40 mg ml<sup>−1</sup>, no crystals were observed. We subjected the protein to limited proteolysis in order to identify protease-resistant domains which might facilitate crystallization. Digestion with the protease subtilisin resulted in two stable cleavage products (Fig. 1b). Analysis by mass spectrometry revealed that ∼50 amino acids were removed from the C-­terminus of the protein. We therefore designed, cloned and expressed a new construct, WbdD556 (residues 1–556), that was purified in the same way as WbdD600. Although this construct was still classified as `very difficult' by XtalPred, it readily crystallized in a Tacsimate-based condition (51% Tacsimate pH 8.0, 5 mM SAM/Mg²⁺/ATP after optimization) based on the commercially available Index screen (condition 29; Hampton Research; Fig. 1c). Condition 2.4 of The JCSG+ Suite (Molecular Dimensions) led to identical WbdD556 crystals growing from a mixture of lithium sulfate and ammonium sulfate (0.26 M lithium sulfate, 0.1 M Tris–HCl pH 8.4, 1.12 M ammonium sulfate, 5 mM SAM/Mg²⁺/ATP after optimization). We found that these crystals could also be obtained by in situ proteolysis (Dong et al., 2007; Wernimont & Edwards, 2009) by crystallizing WbdD600 in the presence of low concentrations (1:1000 molar ratio of protease:WbdD) of subtilisin or trypsin.

3.2. WbdD crystals vary in diffraction quality

The initial optimization of the cubic (space group I23) WbdD crystals was based on their optical appearance and proved to be straightforward; large crystals (>200 µm) could be grown (Fig. 1d). However, these crystals typically did not diffract to a resolution better than 7–8 Å (crystal category D). From over 500 tested crystals, only a dozen diffracted to 4–5 Å resolution (crystal category C) and only three crystals to around 3.5 Å resolution (crystal category B). An SeMet derivative gave similar results. During crystal screening, we noticed that the first crystal retrieved from a drop frequently diffracted significantly better than subsequently harvested crystals. We found a single native crystal that diffracted to better than 3.0 Å resolution (crystal category A), although there was no obvious visual indication as to why and this could not be reproduced. This crystal grew from the ammonium sulfate-based condition, but was harvested more than six months after the plate had been set up. During the intervening time, approximately half of the volume of the reservoir solution had evaporated, leaving a much higher (∼2 M) concentration of ammonium sulfate. By comparing the unit-cell parameters of the different crystal categories (A, B, C and D), we found that the decrease in length of the cubic unit-cell axis from 185 Å in crystal category D to 167 Å in crystal category A generally correlated with resolution. We interpreted this as an indication that dehydration of the WbdD556 crystals improved diffraction quality (Table 1).

3.3. Solving the selenium substructure and determining the correct hand

WbdD556 crystals were sensitive to radiation damage and the highest resolution SeMet data set was processed to 3.5 Å with HKL-2000 (Otwinowski & Minor, 1997; Table 2). The anomalous signal was found to extend to about 4.5 Å resolution using SHELXC (Sheldrick, 2008) and phenix.xtriage (Zwart et al., 2008) (Fig. 2), and five of the expected seven selenium sites were located by SHELXD (Sheldrick, 2008). However, the phasing and density-improvement step in SHELXE (Sheldrick, 2008) did not result in an interpretable map or even clear solvent boundaries in either hand. We repeated the initial phasing steps with phenix.hyss (Grosse-Kunstleve & Adams, 2003; Zwart et al., 2008) and SOLVE/RESOLVE (Terwilliger, 2003) from the PHENIX suite (Adams et al., 2002), but obtained similar results. In the map corresponding to the original hand we noted a long tubular stretch of electron density wound around the crystallographic threefold axis, suggesting a coiled coil (Fig. 3a). No similar structure was visible in the other hand. We inserted a 20-residue helix model using Coot and submitted the resulting crystallographic helical trimer to the DALI and SSM servers (Holm & Rosenström, 2010; Krissinel & Henrick, 2004). Both algorithms identified structures with similar arrangements of helices (e.g. PDB entries 1xkm, 2lfh and 3aha; Raimondo et al., 2005; Northeast Structural Genomics Consortium, unpublished work; Izumi et al., 2010), supporting the notion that the geometric arrangement of helices was reasonable. On this basis, we tentatively selected the hand.

Table 2 Data-collection statistics for WbdD556 The 3.0 Å resolution data set was processed so that all data with I/σ(I) > 1.0 were included. Values in parentheses are for the highest resolution shell   3.0 Å, native 3.5 Å, SeMet 4.2 Å, native Space group I23 I23 I23 Unit-cell parameters (Å, °) a = b = c = 167.2, α = β = γ = 90 a = b = c = 177.6, α = β = γ = 90 a = b = c = 180.0, α = β = γ = 90 Matthews coefficient (Å3 Da−1) 3.1 3.7 3.88 Solvent content (%) 61 67 69 Molecules per asymmetric unit 1 1 1 Resolution range (Å) 30.0–2.80 (2.85–2.80) 55.0–3.52 (3.59–3.52) 63.6–4.22 (4.33–4.22) Total observations 197495 81347 153562 Unique reflections 19185 11716 7119 Completeness (%) 99.5 (97.5) 95.6 (98.3) 100 (100) Rmerge† (%) 10.3 (200) 8 (84) 20 (57.1) Multiplicity 10.3 6.9 21.6 〈I/σ(I)〉 20.1 (1.1) 30.2 (2.2) 14.8 (6.0) †Rmerge = , where Ii(hkl) is the intensity for all observations i of reflection hkl and 〈I(hkl)〉 is the weighted average intensity for all observations i of reflection hkl.

Figure 2 Strength 〈|F+ − F−|/[(σF+)2 − (σF−)2]1/2〉 of the anomalous signal versus resolution for the 3.5 Å resolution data set (Table 2) as calculated using SHELXC (Sheldrick, 2008). The red line at y = 0.8 indicates the threshold for the presence of an anomalous signal (Zwart, 2005).

Figure 3 (a) Stereo pair of an electron-density map (1.0σ) calculated with the starting experimental phases after density improvement with AUTOSOLVE (Zwart et al., 2008). The manually placed three-helix bundle is shown as a yellow tube model; the threefold crystallographic axis is indicated by a red line. (b) The white mesh represents the same map as shown in (a). The purple map was calculated from phases that were improved using PARROT. Manually placed secondary-structure elements are represented by yellow tubes. (c) The purple mesh is the same as in (b). The green map was calculated after cross-crystal averaging between the 3.5 Å resolution data set and the 4.2 Å resolution data set (Table 2) using DMMULTI (Winn et al., 2011). Manually placed secondary-structure elements are represented by yellow tubes. The crystal structure (white tubes) of the SAM-dependent methyltransferase from Pyrococcus horikoshii OT3 (PDB entry 1wzn; RIKEN Structural Genomics/Proteomics Initiative, unpublished work) is superimposed onto the manually placed secondary-structure elements. (d) The green mesh is the same as in (c). The individual domains of WbdD556 are indicated (white, methyltransferase domain; red, kinase domain; yellow, three-helix bundle).

3.4. Additional density improvement to build a crude model

The Parrot program (Winn et al., 2011; Cowtan, 2010) improved the map quality (Fig. 3b). The most significant difference was a better defined solvent boundary, which made it possible to identify the elongated shape of the molecule (Fig. 3b). We also tentatively assigned secondary-structure elements. Our strategy at this point was to improve the model to a point where it would be possible to phase the 3.0 Å resolution native data set by molecular replacement. However this failed, indicating that the model was substantially incorrect. Owing to variations of the unit-cell length, we had a collection of non-isomorphous crystals (Tables 1 and 2) and we decided to try cross-crystal averaging with DMMULTI. We defined a mask around our crude model and cross-crystal averaged using the 3.0 Å resolution native data set, assuming that the mask occupied the same position relative to the threefold axis and allowing the matrix to refine. Unfortunately, the resulting maps were not interpretable. Employing the same procedure with a 4.2 Å resolution data set resulted in a much improved electron-density map (Fig. 3c) and sufficient secondary-structural elements were fitted to confidently identify the MTase domain. This defined which part of the map corresponded to which of the two domains of WbdD. A model of Src kinase could be placed by hand into the remaining density, although the quality of fit was poor (Fig. 3d).

3.5. Molecular replacement with electron density

We suspected that the large changes in the unit-cell parameters were accompanied by domain reorganization within the crystal. We proceeded by using the separate domains for molecular replacement into the native 3.0 Å resolution data set and found a convincing solution for the MTase domain using PHASER (McCoy et al., 2007; TFZ score 16.7, LLG 232), but no solution for the kinase domain was found. Inspection of the maps calculated from the molecular-replacement phases showed no additional difference electron density. This indicated that either the kinase domain was disordered in these crystals or our incorrect model biased the phases. We repeated molecular replacement using the density for each domain (rather than the structure) as the search model in an attempt to avoid model bias as much as possible. Once again, the MTase domain was confidently located by PHASER (McCoy et al., 2007), but multiple borderline solutions were obtained for the kinase domain with low Z-scores of ∼6 and LLGs of around 40. Inspection of the Euler angles and translational shifts of the solutions revealed that the top solution of this set was in close proximity to the MTase domain solution. Phases calculated from the combined top solutions then resulted in electron density that was interpretable for both domains (Fig. 4). We were able to confidently build a complete medium-resolution model of WbdD and refine it by simulated annealing (R = 21.2%, R_free = 23.3%; Fig. 5, Table 3).

Table 3 Refinement statistics for WbdD556 The structure has been deposited in the PDB as entry 4ax8.   3.0 Å, native R (%) 21.2 Rfree (%) 23.3 R.m.s.d. bonds (Å) 0.017 R.m.s.d. angles (°) 1.835 Ramachandran plot (%)  Allowed 97.3  Disallowed 0.2 MolProbity† score 1.86 MolProbity† clashscore 7.01 †Chen et al. (2010).

Figure 4 Electron-density map (blue, 2m|Fobs| − D|Fcalc|, 1.0σ; green, m|Fobs| − D|Fcalc|, 3.0σ; red, m|Fobs| − D|Fcalc|, −3.0σ) calculated from phases directly after the masked electron densities shown in Fig. 3(c) were used as a model for molecular replacement in the 3.0 Å resolution data set (Table 2). The WbdD model (yellow) and the SAM cofactor (white) are shown as sticks.

Figure 5 Cartoon model of the overall structure of a WbdD trimer. One monomer is coloured green and the other two are shown in grey. The relative positions of the MTase and kinase domains are indicated.

3.6. A new rapid dehydration method

Only after the preceding approaches resulted in a solved medium-resolution structure of WbdD were we able to develop a protocol that would give reproducible high-quality crystals for further study.

To investigate the role of crystal dehydration in determining data quality, we mounted crystals in a free-mounting system (FMS; Proteros; Kiefersauer et al., 2000) and lowered the relative humidity (r.h.) of the gas phase surrounding the crystal. During this process, diffraction snapshots were taken and these revealed a clear improvement in the diffraction quality when the r.h. was lowered to ∼86% (Fig. 6). Indexing of the individual diffraction images revealed that in the initial stage (∼90% r.h.) the unit-cell parameter decreased (from 174 to 168 Å) and the diffraction quality improved (Fig. 6). Further dehydration (∼86% r.h.) led to only a slightly decreased unit-cell size but significantly improved the diffraction quality. The change in unit-cell parameters renders the crystals non-isomorphous. Below 86% r.h. the diffraction quality deteriorated and the process was not reversible. This is reminiscent of the complex dehydration behaviour observed in HIV-1 reverse transcriptase crystals (Esnouf et al., 1998). WbdD556 crystals often cracked during dehydration with the FMS, degrading the diffraction quality. Covering the crystal in perfluoropolyether oil, setting the r.h. to 92% and allowing the dehydration to proceed overnight proved to be a reproducible procedure.

Figure 6 Right to left: dehydration of a WbdD556 crystal using a free-mounting system (Kiefersauer et al., 2000). As indicated, the diffraction images were taken at different relative humidities (r.h.). The images were indexed with HKL-2000 (Otwinowski & Minor, 1997) to analyze the change in the cubic unit-cell parameter.

From a practical perspective, however, this procedure was not suited for the production of multiple crystals. We therefore tested a commercially available dehydration kit (JBS Crystal Dehydration and Salvage Kit), in which the r.h. is controlled by a salt solution inside a cap which covers a mounted crystal. This was unsuccessful either because the salt often crystallized or because mechanical errors in placing the cap destroyed the crystal. A different approach was devised that involved filling the reservoirs of a 96-well crystallization plate (MRC plate) with different saturated salt solutions (e.g. potassium nitrate, ammonium sulfate, sodium chloride and magnesium chloride). Using a cryoloop (MiTeGen), crystals were then placed into 0.5 µl perfluoropolyether oil drops (one crystal per drop) in each well (Fig. 7). A cat whisker was used to push the crystal out of the cryoloop into the oil drop if required. The plate was then sealed and incubated for one week. Crystals were harvested from the oil drop and cryocooled in liquid nitrogen prior to the diffraction experiment. With WbdD, dehydration with ammonium sulfate (r.h. 81%) reproducibly yielded crystals that diffracted to better than 2.5 Å resolution. The best diffracting crystals (which also belonged to space group I23) had a unit-cell parameter of 158 Å, which is again considerably shorter than the 167 Å unit-cell parameter of the native crystal that was used to solve the initial structure (Table 2). The resulting high-resolution structure, complexes, mutants and biological implications will be described elsewhere.

Figure 7 Schematic of our novel crystal-dehydration workflow. (a) A 96-well plate was filled with different saturated salt solutions. (b) The crystal (red) was harvested from its mother liquor and placed in a drop of perfluoro­polyether oil (PFO-X175/08; Hampton Research) on the sitting-drop shelf. The plate was then sealed and the setup was kept at room temperature for a week before the crystals were harvested and flash-cooled using liquid nitrogen prior to data collection.

4.1. Effect of dehydration on the structure

After the structure of WbdD556 had been solved as described above, the model was used to solve the structure of the 4.2 Å low-resolution data set (Table 2) in order to investigate the structural changes that occurred during dehydration. The RAPIDO webserver was employed to align the two structures (Mosca & Schneider, 2008; Mosca et al., 2008). The algorithm detected two rigid bodies in WbdD. The first one comprises residues 7–194, 203–230, 239–259, 264–314, 340–351 and 368–376 and represents the MTase domain together with the N-­lobe of the kinase domain (Fig. 8). The second rigid body contains the C-lobe of the kinase domain (residues 315–339, 352–367, 383–398 and 418–449). Superposing both structures based on the first rigid body revealed a twisting motion with a hinge between the N- and C-lobes of the kinase domain (ATP-binding cleft). In the highest resolution (better than 2.5 Å) WbdD structures the ATP added to the crystallization conditions was disordered. In these structures, the twisting motion displaces the nucleotide from the binding pocket by inserting protein residues into the binding cleft. There is a 20° movement of the C-terminal helix that forms the three-helix bundle in the trimer. In the high-resolution structure this helix is in much closer contact with the kinase domain (Fig. 8). In retrospect, the rigid-body motion within the kinase domain probably explains why molecular replacement based on a single kinase domain failed. The structural changes are striking when viewed in the crystal-packing environment (Fig. 9). Four WbdD trimers organize themselves into a pyramid-shaped arrangement that is centred on each corner of a unit cell. During dehydration, this assembly is dramatically compressed as the three arms of the trimers bind to each other. This may explain why dehydration is so reproducible in yielding good-quality crystals (Fig. 9).

Figure 8 (a) Rigid bodies (yellow and blue ribbons) in WbdD556 identified by the RAPIDO webserver (Mosca & Schneider, 2008). Hinge regions are shown as red ribbons and the cofactors ATP and SAM as spheres. (b) Superposition of the 3.0 Å resolution (red) and 4.2 Å resolution (green) WbdD structures (Table 2) showing the twisting motion that occurs during crystal dehydration and pivots around the ATP-binding cleft. The superposition is based on the rigid body that comprises the methyltransferase and the N-lobe of the kinase domain [coloured yellow in (a)].

Figure 9 Effect of dehydration on the trimeric WbdD structure and the crystal packing. The top row shows the packing of the non-dehydrated crystal and the bottom row that of the dehydrated crystal. One of the four WbdD556 trimers (surface representation) which are centred around each unit-cell corner is shown in blue with a superimposed cartoon representation of the protein (white). In the figure at the top right, the pyramid-shaped assembly shown on the left is cut open to reveal the size of the internal hollow space.

We analyzed the two crystal-packing arrangements (dehydrated and non-dehydrated) using the PISA server (Krissinel & Henrick, 2007). In the non-dehydrated form each WbdD556 monomer with a surface area of ∼21 000 Ų contributes to three protein–protein interfaces. Each of these has a relatively small interface area of ∼200 Ų formed by 6–8 amino-acid residues. After dehydration, each WbdD556 monomer is involved in five protein–protein interfaces; four of these have interface areas between 400 and 700 Ų and involve up to 26 amino-acid residues at each interface. In the dehydrated state, the interactions between WbdD556 monomers are so extensive that PISA classifies both the whole pyramid-shaped assembly (Fig. 9) and the trimer as being stable in solution. In contrast, PISA did not define any of the interfaces in the `non-dehydrated' packing as being stable in solution. This included the trimer, which can be identified in solution by gel filtration (data not shown).

Each of the WbdD556 structures contains an ∼20-residue α-­helix remaining from the C-terminal coiled-coil domain (Fig. 1a). In the lower-resolution structures we could model 5–6 additional residues of the helix, but the remaining ∼80 residues were always disordered. For four WbdD556 trimers in one pyramid (Fig. 9), almost 1000 amino acids would have to fit into the central cavity. A simple volume calculation revealed that the void is indeed large enough to accommodate the missing residues unless the remaining residues formed a single helix. The volume of the cavity shrinks during dehydration, perhaps explaining why in contrast to the rest of the structure the helical bundle becomes more disordered as the crystals are dehydrated. The void is too small for constructs that have a longer C-terminal bundle than WbdD556 (Fig. 9).

More than ten years ago, at the outset of structural genomics (SG) programs, our laboratory solved the structure of UDP-galactopyranose mutase (Sanders et al., 2001). This project represented a challenge owing to varying crystal quality and non-isomorphism between phased and unphased diffraction data sets. Despite the transformation in infrastructures (e.g. beamlines) and software packages in the intervening years, very similar problems were encountered with WbdD. One key element in the process of solving the structure of WbdD was the discovery of a plausible three-helix bundle in one low-resolution electron-density map (Fig. 3a). The quantity of the structural data in the PDB made this possible. Density-modification software such as DMMULTI (Winn et al., 2011) and PARROT (Cowtan, 2010) was essential in solving the structure of WbdD. In a counter-intuitive outcome, dramatically improved electron density was achieved by cross-crystal averaging with the non-isomorphous low-resolution (but not the high-resolution) data set (4.2 Å resolution; Table 2; Fig. 3c). Use of electron density as a molecular-replacement model with, for example, PHASER (McCoy et al., 2007) is now much more automated and this was also critical to our success. Although multiple cases are known in which crystals were successfully dehydrated to improve their diffraction properties (see, for example, Esnouf et al., 1998; Kiefersauer et al., 2000; Chotiyarnwong et al., 2007), it is still a technique that is rarely reported. This perhaps reflects frequent failure or technical difficulties. WbdD may be a special case because the high symmetry (I23) of the crystal lattice leads to isotropic changes in the crystal and the novel packing arrangement was particularly favourable (Fig. 9). The effect of dehydration on WbdD (an improvement in diffraction resolution from 8 to 2.2 Å) is very pronounced when compared with other examples (Heras & Martin, 2005). However, a facile and easily implementable protocol may extend the utility of dehydration. The procedure described here combines several previously published ideas (Kiefersauer et al., 2000; Heras & Martin, 2005) into an easy-to-use, low-cost and convenient workflow. It is not anticipated that this will necessarily lead to more success than other approaches, but it is straightforward and amenable to high throughput. With robotic mounting and testing of samples, the technique may be appropriate for routine application for poorly diffracting high-solvent crystals.