Does Calretinin Immunohistochemistry Reduce Inconclusive Diagnosis in Rectal Biopsies for Hirschsprung Disease?
Corresponding Author
Pedro L.T. de Arruda Lourenção
Division of Pediatric Surgery, Department of Surgery, Botucatu Medical School, UNESP—Universidade Estadual Paulista, Botucatu-SP, Brazil
Address correspondence and reprint requests to Prof Pedro L.T. de Arruda Lourenção, Division of Pediatric Surgery, Department of Surgery, Botucatu Medical School, UNESP–São Paulo State University, Botucatu-SP CEP 18618-970, Brazil (e-mail: [email protected]).Search for more papers by this authorBonifácio K. Takegawa
Division of Pediatric Surgery, Department of Surgery, Botucatu Medical School, UNESP—Universidade Estadual Paulista, Botucatu-SP, Brazil
Search for more papers by this authorErika V.P. Ortolan
Division of Pediatric Surgery, Department of Surgery, Botucatu Medical School, UNESP—Universidade Estadual Paulista, Botucatu-SP, Brazil
Search for more papers by this authorSimone A. Terra
Department of Pathology, Botucatu Medical School, UNESP—Universidade Estadual Paulista, Botucatu-SP, Brazil
Search for more papers by this authorMaria A.M. Rodrigues
Department of Pathology, Botucatu Medical School, UNESP—Universidade Estadual Paulista, Botucatu-SP, Brazil
Search for more papers by this authorCorresponding Author
Pedro L.T. de Arruda Lourenção
Division of Pediatric Surgery, Department of Surgery, Botucatu Medical School, UNESP—Universidade Estadual Paulista, Botucatu-SP, Brazil
Address correspondence and reprint requests to Prof Pedro L.T. de Arruda Lourenção, Division of Pediatric Surgery, Department of Surgery, Botucatu Medical School, UNESP–São Paulo State University, Botucatu-SP CEP 18618-970, Brazil (e-mail: [email protected]).Search for more papers by this authorBonifácio K. Takegawa
Division of Pediatric Surgery, Department of Surgery, Botucatu Medical School, UNESP—Universidade Estadual Paulista, Botucatu-SP, Brazil
Search for more papers by this authorErika V.P. Ortolan
Division of Pediatric Surgery, Department of Surgery, Botucatu Medical School, UNESP—Universidade Estadual Paulista, Botucatu-SP, Brazil
Search for more papers by this authorSimone A. Terra
Department of Pathology, Botucatu Medical School, UNESP—Universidade Estadual Paulista, Botucatu-SP, Brazil
Search for more papers by this authorMaria A.M. Rodrigues
Department of Pathology, Botucatu Medical School, UNESP—Universidade Estadual Paulista, Botucatu-SP, Brazil
Search for more papers by this authorThe authors report no conflicts of interest.
ABSTRACT
Many difficulties occur during the evaluation of rectal biopsies for the diagnosis of Hirschsprung disease. We investigated whether the introduction of calretinin (CR) immunohistochemistry in a diagnostic panel could decrease the rate of inconclusive results. Data from 82 patients undergoing rectal biopsies before and after CR introduction were analyzed. Inconclusive results were obtained in 17 of 45 rectal biopsies (37.8%) in the series of cases before CR introduction and in 5 of 42 rectal biopsies (11.9%) in the series of cases after CR (P < 0.006). The inclusion of CR in the histopathologic panel may improve the diagnostic accuracy of Hirschsprung disease.
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