Comparative In Vitro Evaluation of Two Different Preparations of Small Diameter Polyurethane Vascular Grafts

Abstract: Polyurethane (PU) frequently has been used to manufacture small diameter vascular grafts due to its good bicompatibility. In this study, sponge PU small diameter vascular grafts were fabricated from Pellethane 2103, as well as a self-synthesized PU, by utilization of a salt casting technique and by varying the salt/polymer ratios. Two types were made; one of them had a thin solid layer on the outer surface. The inner surface was identical and was coated with gelatin crosslinked by epoxide. Tensile properties, compliance, platelet activation, and endothelial cell attachment were evaluated in vitro. It was found that for the grafts with an outer nonporous coating, compliance could not be estimated from modulus due to anisotropy. The most compliance-matched grafts were made of self-synthesized polyurethane with a salt/polymer ratio 4 to 8 or Pellethane with a ratio 6 to 8 without the outer nonporous coating. The former had better elongation. Self-synthesized PU had lower platelet adhesion as well as more endothelial attachment than Pellethane. Porosity activated platelets strongly and reduced endothelial adhesion unless the surface was modified by the crosslinked gelatin layer. It was concluded that PU synthesized in our lab with a salt/polymer ratio of at least 4 and coated with epoxy crosslinked gelatin was a better substrate for preparation of small diameter vascular grafts.