Polymorphism of HLA-A, -B, -DRB1, -DQA1 and -DQB1 haplotypes in a Croatian population
Corresponding Author
Z. Grubic
National Referral Organ Transplantation and Tissue Typing Center, University Hospital Zagreb, Kispatic eva 12, 10000 Zagreb, Croatia
Zorana Grubic , National Referral Organ Transplantation and Tissue Typing Center, University Hospital Zagreb, Kispaticeva 12, 10000 Zagreb, Croatia.Search for more papers by this authorR. Z unec
National Referral Organ Transplantation and Tissue Typing Center, University Hospital Zagreb, Kispatic eva 12, 10000 Zagreb, Croatia
Search for more papers by this authorE. C ec uk-Jelic ic
National Referral Organ Transplantation and Tissue Typing Center, University Hospital Zagreb, Kispatic eva 12, 10000 Zagreb, Croatia
Search for more papers by this authorV. Kerhin-Brkljac ic
National Referral Organ Transplantation and Tissue Typing Center, University Hospital Zagreb, Kispatic eva 12, 10000 Zagreb, Croatia
Search for more papers by this authorA. Kas telan
National Referral Organ Transplantation and Tissue Typing Center, University Hospital Zagreb, Kispatic eva 12, 10000 Zagreb, Croatia
Search for more papers by this authorCorresponding Author
Z. Grubic
National Referral Organ Transplantation and Tissue Typing Center, University Hospital Zagreb, Kispatic eva 12, 10000 Zagreb, Croatia
Zorana Grubic , National Referral Organ Transplantation and Tissue Typing Center, University Hospital Zagreb, Kispaticeva 12, 10000 Zagreb, Croatia.Search for more papers by this authorR. Z unec
National Referral Organ Transplantation and Tissue Typing Center, University Hospital Zagreb, Kispatic eva 12, 10000 Zagreb, Croatia
Search for more papers by this authorE. C ec uk-Jelic ic
National Referral Organ Transplantation and Tissue Typing Center, University Hospital Zagreb, Kispatic eva 12, 10000 Zagreb, Croatia
Search for more papers by this authorV. Kerhin-Brkljac ic
National Referral Organ Transplantation and Tissue Typing Center, University Hospital Zagreb, Kispatic eva 12, 10000 Zagreb, Croatia
Search for more papers by this authorA. Kas telan
National Referral Organ Transplantation and Tissue Typing Center, University Hospital Zagreb, Kispatic eva 12, 10000 Zagreb, Croatia
Search for more papers by this authorAbstract
We describe for the first time extended haplotypes in a Croatian population. The present study gives the HLA-A, -B, -DRB1, -DQA1 and -DQB1 allele and haplotype frequencies in 105 families with at least two offspring. All individuals were studied by conventional serology for HLA class I antigens (A and B), while class II alleles (DRB1, DQA1, DQB1) were typed using the PCR–SSOP method. HLA genotyping was performed by segregation in all 105 families. For extended haplotype analysis, 420 independent parental haplotypes were included. Fourteen HLA-A, 18 HLA-B, 28 DRB1, 9 DQA1 and 11 DQB1 alleles were found in the studied population. Most of the DRB1 alleles in our population had an exclusive association with one specific DQA1-DQB1 combination. This strong linkage disequilibrium within the HLA class II region is often extended to the HLA-B locus. A total of 10 HLA-A, -B, -DRB1, -DQA1, -DQB1 haplotypes were observed with a frequency ≤ 1.0%. The three most frequent haplotypes were HLA-A1, B8, DRB1*0301, DQA1*0501, DQB1*0201; HLA-A3, B7, DRB1*1501, DQA1*0102, DQB1*0602 and HLA-A24, B44, DRB1*0701, DQA1*0201, DQB1*02. These results should provide a useful reference for further anthropological studies, transplantation studies, and studies of associations between HLA and diseases.
References
- Begovich, A.B., McClure, G.R., Suraj, V.C., Helmuth, R.C., Fildes, N., Bugawan, T.L., Ezlich, H.A., Klitz, W. (1992) Polymorphism, recombination, and linkage disequlibrium within the HLA class II region. The Journal of Immunology, 148, 249.
- Bignon, J.D., Cheneau, M.L., Herry, P., Bonneville, F., Cesborn, A., Muller, J.Y. (1992) Strong linkage disequlibrium of HLA DPw11 with the HLA B44–DR7-DQw2 extended haplotype. Tissue Antigens, 39, 35.
- Bignon, J.D. & Fernandez, M.A. (1997) Protocols of the 12th International Histocompatibility Workshop for typing of HLA class II alleles by DNA amplification by the polamerase chain reaction (PCR) and hybridization with sequnce specific oligonucleotide probes (SSOP). In: Genetic Diversity of HLA Functional and Medical Implications. Proceedings of the Twelfth International Histocompatibility Workshop and Conference, Vol. 1 (ed. by D. Charron), p. 584. EDK, Paris.
- Bodmer, J.G., Marsh, S., Albert, E.D., Bodmer, W.F. (1997) Nomenclature for factors of the HLA system, 1996& thinsp;— updates January, February, March, April 1997. In: Genetic Diversity of HLA Functional and Medical Implication. Proceedings of the Twelfth International Histocompatibility Workshop and Conference, Vol. 1 (ed. by D. Charron), p. 505. EDK, Paris.
- Cerna, M., Fernandez-Vina, M.A., Ivaskova, E., Stastny, P. (1992) Comparison of HLA class II alleles in Gypsy and Czech populations by DNA typing with oligonucleotide probes. Tissue Antigens, 39, 111.
- Cuccia, M., Astolfi, P., Gyodi, E., Kas telan , A. (1992) HLA in five populations of southern Europe. In: HLA 1991. Proceedings of the Eleventh International Histocompatibility Workshop and Conference, Vol. 1 (ed. by K. Tsuji, M. Aizawa & T. Sasazuki), p. 656. Oxford University Press, Oxford.
- Degos, L. & Dausset, J. (1974) Human migrations and linkage disequilibrium of HLA system. Immunogenetics, 1, 195.
- Fernandez-Vina, M.A., Gao, X., Moares, M.E., Moares, J.R., Salatiel, I., Miller, S., Tsai, J., Sun, Y., An, J., Layrisse, Z., Gazit, E., Brauthar, C., Stastry, P. (1991) Alleles at four HLA class II loci determined by oligonucleotide hybridization and their associations in five ethnic groups. Immunogenetics, 34, 299.
- Fisher, R.A. (1973) Statistical Methods for Research Workers. Hafner Publishing Company, New York.
- Gregersen, P.K., Kao, H., Nunex-Roland, A., Hurley, C.K., Karr, R.W., Silver, J. (1988) Recombination sites in the HLA class II region are haplotype dependent. Immunogenetics, 18, 503.
- Grubic , Z., Z unec , R., Kas telan , A. (1996) Striking diversity of DR15 haplotypes in Croatians. Tissue Antigens, 49, 180.
- Grubic , Z., Z unec , R., Naipal, A., Kas telan , A., Giphart, M.J. (1995) Molecular analysis of HLA class II polymorphism in Croatians. Tissue Antigens, 46, 293.
- Imanishi, T., Akaza, T., Kimura, A., Tokunaga, K., Gojobori, T. (1992a) Estimation of allele and haplotype frequencies for HLA and complement loci. In: HLA 1991. Proceedings of the Eleventh International Histocompatibility Workshop and Conference, Vol. 1 (eds by K. Tsuji, M. Aizawa & T. Sasazuki), p. 76. Oxford University Press, Oxford.
- Imanishi, T., Akaza, T., Kimura, A., Tokunaga, K., Gojobori, T. (1992b) Allele and haplotype frequencies for HLA and complement loci in various ethnic groups. In: HLA 1991. Proceedings of the Eleventh International Histocompatibility Workshop and Conference, Vol. 1 (eds by K. Tsuji, M. Aizawa & T. Sasazuki), p. 1065. Oxford University Press, Oxford.
- Ivanova, R., Naoumova, E., Lepage, V., Djoulah, S., Yordanov, Y., Loste, M.N., Charron, D. (1996) HLA-DRB1, DQA1, DQB1 DNA polymorphism in the Bulgarian population. Tissue Antigens, 47, 122.
- Jungerman, M., Sanchez-Mazas, A., Fichna, P., Ivanova, R., Charron, D. (1997) HLA class II DRB1, DQA1 and DQB1 polymorphism in the Polish population from Wielkopolska. Tissue Anitigens, 49, 624.
- Kas telan , A., Kerhin-Brkljac ic , V., Hors, J., Brkljac ic , L., Macasevic, P. (1974) The distribution of HLA antigens and genes in the Yugoslav population. Tissue Antigens, 4, 69.
- Kimura, A. & Sasazuki, T. (1992) Eleventh international histocompatibility workshop reference protocol for the HLA DNA-typing technique. In: HLA 1991. Proceedings of the Eleventh International Histocompatibility Workshop and Conference, Vol. 1 (eds by K. Tsuji, M. Aizawa & T. Sasazuki), p. 1065. Oxford University Press, Oxford.
- Krokowski, M., Bodalski, J., Bratek, A., Boitard, C., Caillant-Zucman, S. (1998) HLA class II allele and haplotype distribution in a population from central Poland. European Journal of Immunogenetics, 25, 5.
- Martinez-Laso, J., De Juan, D., Martinez-Quiles, M., Gomez-Casado, E., Cuadrado, E., Arnaiz-Villena, A. (1995) The contribution of the HLA-A, -B, -Cw and -DR, -DQ DNA typing to the study of the origins of Spaniards and Basques. Tissue Antigens, 45, 237.
- Martinez-Laso, J., Gazit, E., Gomez-Casado, E., Morales, P. (1996) HLA-DR and DQ polymorphism in Ashkenazi and non-Ashkenazi Jews: comparison with other Mediterraneans. Tissue Antigens, 47, 63.
- Matsui, Y., Alosco, S.M., Awdeh, Z. (1984) Linkage disequlibrium of HLA-SB1 with HLA-A1, B8, DR3, SCO1 and HLA–SB4 with the HLA–A26, Bw38, Dw10, DR4, SC21 extended haplotypes. Immunogenetics, 20, 623.
- Miller, S.A., Dykes, D.D., Polesky, H.F. (1988) A simply salting-out procedure for extracting DNA from human nucleated cells. Nucleic Acids Research, 16, 1215.
- Piazza, A., Fauchet, R., Richiardi, P., Carcassi, C., Contu, T. (1992) Anthropology report: Belgium, French, Italian, Portuguese, Sardinian, and Spanish populations. In: HLA 1991. Proceedings of the Eleventh International Histocompatibility Workshop and Conference, Vol. 1 (eds by K. Tsuji, M. Aizawa & T. Sasazuki), p. 648. Oxford University Press, Oxford.
- Ray, J.G. (1979) NIAID Manual of Tissue Typing Techniques, 1979, 39. NIH Publication no. 80. Bethesda.
- Ronningen, K.S., Spurkland, A., Iwe, T., Vartdal, F., Thonsby, E. (1991) Novel HLA-DR2 and -DR3 haplotypes among Norwegian Caucasians. Tissue Antigens, 37, 165.
- Stavropoulos-Giokas, C., Papasteriades, C., Polymemenidis, A., Naumova, E., Sruhan, G., Varnavidou, A. (1997) HLA in MEDI Region populations: 12th International Histocompatibility Workshop MEDI. In: Genetic Diversity of HLA Functional and Medical Implications. Proceedings of the Twelfth International Histocompatibility Workshop and Conference, Vol. 1 (ed. by D. Charron), p. 323. EDK, Paris.
- Tiilikainen, A., Fischer, G., Grubic , Z., Gyodi, E. (1997) Anthropological features of the East European region. In: Genetic Diversity of HLA Functional and Medical Implications. Proceedings of the Twelfth International Histocompatibility Workshop and Conference, Vol. 1 (ed. by D. Charron), p. 307. EDK, Paris.
- Verduyin, W., Doxiadis, I., Anthols, J., Drabbels, J.J.M., Haipal, A., D’Amano, J., Persijn, G., Giphard, M.J., Schreuber, G.M.TH. (1993) Biotynlated DRB sequence specific oligonucleotides: comparison to serological HLA-DR typing of organ donors in Eurotransplant. Human Immunology, 37, 59.
