Influence of anti-CD49f and anti-CD29 monoclonal antibodies on mitotic activity of epithelial cells (HaCaT) and gingival fibroblasts in vitro
Christoph Becker
Department for Operative Dentistry, Periodontology and Preventive Dentistry, Medical Faculty, Rheinisch Westfälische Technische Hochschule (RWTH) Aachen, Germany,
Interdisciplinary Center of Clinical Research in Biomaterials and Tissue–Material Interaction in Implants ‘BIOMAT.’, Medical Faculty, RWTH Aachen, Germany
Search for more papers by this authorPatricia Buttler
Department for Operative Dentistry, Periodontology and Preventive Dentistry, Medical Faculty, Rheinisch Westfälische Technische Hochschule (RWTH) Aachen, Germany,
Search for more papers by this authorHans Georg Gräber
Department for Operative Dentistry, Periodontology and Preventive Dentistry, Medical Faculty, Rheinisch Westfälische Technische Hochschule (RWTH) Aachen, Germany,
Search for more papers by this authorChristoph Becker
Department for Operative Dentistry, Periodontology and Preventive Dentistry, Medical Faculty, Rheinisch Westfälische Technische Hochschule (RWTH) Aachen, Germany,
Interdisciplinary Center of Clinical Research in Biomaterials and Tissue–Material Interaction in Implants ‘BIOMAT.’, Medical Faculty, RWTH Aachen, Germany
Search for more papers by this authorPatricia Buttler
Department for Operative Dentistry, Periodontology and Preventive Dentistry, Medical Faculty, Rheinisch Westfälische Technische Hochschule (RWTH) Aachen, Germany,
Search for more papers by this authorHans Georg Gräber
Department for Operative Dentistry, Periodontology and Preventive Dentistry, Medical Faculty, Rheinisch Westfälische Technische Hochschule (RWTH) Aachen, Germany,
Search for more papers by this authorAbstract
A major complication in the treatment of periodontitis marginalis is the reepithelization of periodontal defects inhibiting collagen fiber attachment and periodontal regeneration. In this study we investigated the possibility of a molecular blockade of epithelial mitosis in vitro. Monoclonal antibodies against the VLA-6 laminin receptor subunit α6 interrupted interactions between epithelial cells (HaCaT cells) and their extracellular matrix and thus resulted in reduction of proliferation rates by more than 50%. The same effect was observed with antiα1-antibodies. In contrast, collagen-producing and -secreting gingival fibroblasts, which play an important role in periodontal regeneration, remained unaffected by the applied antiα6 antibodies. Correspondingly, these cells were found to lack VLA-6 laminin receptors. Selective molecular inhibition of epithelial proliferation and apical migration by monoclonal antiα6 antibody application may provide an adjuvant periodontitis therapy resulting in an enhanced periodontal regeneration.
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