Identification of a new HLA-B*39 allele: HLA-B*3924
Correspondence to:Julia CookTransplantation ImmunologyNuffield Department of SurgeryOxford Transplant CentreChurchill HospitalOxford, OX3 7LJUKFax: +44 1865 226162e-mail:
[email protected]
Abstract:
We have identified a new HLA-B*39 allele through polymerase chain reaction (PCR) using sequence-specific primers (SSP) and sequence-based typing of exons 2 and 3. This novel allele was identified in three HLA-identical siblings of Turkish origin. This allele only differs from HLA-B*3903 at a unique single nucleotide substitution (T for C) at position 365 in exon 3 which results in an amino acid change in codon 98 of methionine (ATG) to threonine (ACG). The sequencing enabled the development of a monospecific PCR-SSP reaction which can be used to discriminate between HLA-B*3924 and other B*39 alleles.
