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Expression of urokinase-type plasminogen activator and its receptor is up-regulated during tendon healing

Wei Xia

Orthopaedic Research Institute, The St. George Hospital, University of New South Wales, Kogarah, Sydney, NSW 2217, Australia

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Charles de Bock,

Charles de Bock

Orthopaedic Research Institute, The St. George Hospital, University of New South Wales, Kogarah, Sydney, NSW 2217, Australia

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George A. C. Murrell,

Corresponding Author

George A. C. Murrell

[email protected]

Orthopaedic Research Institute, The St. George Hospital, University of New South Wales, Kogarah, Sydney, NSW 2217, Australia

Orthopaedic Research Institute, The St. George Hospital, University of New South Wales, Kogarah, Sydney, NSW 2217, Australia. Tel.: +61-2-93502827; fax: +61-293503967Search for more papers by this author

Yao Wang,

Yao Wang

Orthopaedic Research Institute, The St. George Hospital, University of New South Wales, Kogarah, Sydney, NSW 2217, Australia

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Wei Xia,

Wei Xia

Orthopaedic Research Institute, The St. George Hospital, University of New South Wales, Kogarah, Sydney, NSW 2217, Australia

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Charles de Bock,

Charles de Bock

Orthopaedic Research Institute, The St. George Hospital, University of New South Wales, Kogarah, Sydney, NSW 2217, Australia

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George A. C. Murrell,

Corresponding Author

George A. C. Murrell

[email protected]

Orthopaedic Research Institute, The St. George Hospital, University of New South Wales, Kogarah, Sydney, NSW 2217, Australia

Yao Wang,

Yao Wang

Orthopaedic Research Institute, The St. George Hospital, University of New South Wales, Kogarah, Sydney, NSW 2217, Australia

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First published: 01 January 2006

https://doi.org/10.1016/S0736-0266(03)00058-5

Citations: 10

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Abstract

The urokinase-type plasminogen activator (uPA) system has been implicated in cell migration, angiogenesis, extracellular matrix synthesis and tissue remodelling. However, little is known about the role of the system in tendon healing. We used a rat Achilles tendon model to study mRNA and protein expression of uPA and its receptor (uPAR) during tendon healing using immunohistochemical, Northern and Western blot analyses. Time-dependent increases in uPA/uPAR mRNAs and proteins, maximal on days 4–7 and 7–14 respectively, were found following Achilles tendon division. Interestingly, uninjured control tendons expressed uPA mRNA and protein, but little uPAR transcripts and protein. On day 14 following Achilles tendon division, uPA receptor protein increased 12.6-fold (p < 0.001) while uPA itself increased only 1.3-fold (p < 0.05). Immunohistochemical analyses revealed that both uPAR/uPA positive staining cells were increased in the healing tendon tissue section. These findings show for the first time that uPA, and especially its receptor uPAR, are up-regulated during tendon healing. © 2003 Orthopaedic Research Society. Published by Elsevier Science Ltd. All rights reserved.

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Volume21, Issue5

September 2003

Pages 819-825

Expression of urokinase-type plasminogen activator and its receptor is up-regulated during tendon healing

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Expression of urokinase-type plasminogen activator and its receptor is up-regulated during tendon healing

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