Effects of oophorectomy on functional properties of resistance arteries isolated from the cancellous bone of the rabbit femur
Corresponding Author
Vibeke B. Hansen
Department of Obstetrics and Gynaecology, University of Aarhus, Skejby Hospital, Research Laboratory, 8200 Aarhus N, Denmark
Department of Obstetrics and Gynaecology, University of Aarhus, Skejby Hospital, Research Laboratory, 8200 Aarhus N, Denmark, Tel.: +45-8949-6391; fax: +45-8949-6373Search for more papers by this authorAxel Forman
Department of Obstetrics and Gynaecology, University of Aarhus, Skejby Hospital, Research Laboratory, 8200 Aarhus N, Denmark
Search for more papers by this authorAnnette Lundgaard
Department of Orthopaedics, University of Aarhus, Aarhus, Denmark
Institute of Experimental Clinical Research, University of Aarhus, Aarhus, Denmark
Search for more papers by this authorChristian Aalkjaer
Department of Pharmacology, University of Aarhus, Aarhus, Denmark
Search for more papers by this authorKristjar Skajaa
Department of Obstetrics and Gynaecology, University of Aarhus, Skejby Hospital, Research Laboratory, 8200 Aarhus N, Denmark
Search for more papers by this authorEbbe S. Hansen
Department of Orthopaedics, University of Aarhus, Aarhus, Denmark
Institute of Experimental Clinical Research, University of Aarhus, Aarhus, Denmark
Search for more papers by this authorCorresponding Author
Vibeke B. Hansen
Department of Obstetrics and Gynaecology, University of Aarhus, Skejby Hospital, Research Laboratory, 8200 Aarhus N, Denmark
Department of Obstetrics and Gynaecology, University of Aarhus, Skejby Hospital, Research Laboratory, 8200 Aarhus N, Denmark, Tel.: +45-8949-6391; fax: +45-8949-6373Search for more papers by this authorAxel Forman
Department of Obstetrics and Gynaecology, University of Aarhus, Skejby Hospital, Research Laboratory, 8200 Aarhus N, Denmark
Search for more papers by this authorAnnette Lundgaard
Department of Orthopaedics, University of Aarhus, Aarhus, Denmark
Institute of Experimental Clinical Research, University of Aarhus, Aarhus, Denmark
Search for more papers by this authorChristian Aalkjaer
Department of Pharmacology, University of Aarhus, Aarhus, Denmark
Search for more papers by this authorKristjar Skajaa
Department of Obstetrics and Gynaecology, University of Aarhus, Skejby Hospital, Research Laboratory, 8200 Aarhus N, Denmark
Search for more papers by this authorEbbe S. Hansen
Department of Orthopaedics, University of Aarhus, Aarhus, Denmark
Institute of Experimental Clinical Research, University of Aarhus, Aarhus, Denmark
Search for more papers by this authorAbstract
Information is sparse concerning the effect of oophorectomy (OOX) on bone vascularization and blood flow of possible significance for altered remodeling. Whether OOX affects functional characteristics of isolated bone resistance arteries was investigated. Ring preparations (diameter ∼250 μm) of small femoral bone arteries from oophorectomized and sham-operated rabbits were mounted on a myograph six weeks postoperatively. Cumulative concentration-response curves were obtained for various agonists at a normalized lumen diameter. Oophorectomy did not significantly influence lumen diameter, maximal response to high potassium, or maximal response to high potassium and 10−5 M noradrenaline. However, OOX significantly increased the maximal response to noradrenaline (OOX 2.14 ± 0.36 N/m, Sham 1.25 ± 0.14 N/m) and endothelin-1 (OOX 1.76 ± 0.32, Sham 0.95 ± 0.15) in meta-physeal arteries. Moreover, the corresponding maximal active pressure for the agonists was significantly increased. OOX did not influence endothelial function assessed by the effects of acetylcholine or substance P. The functional responses of diaphyseal arteries were unaffected by OOX. The study demonstrates regional differences in the effects of OOX on small arteries of importance for control of vascular resistance in bone which suggests a relation between altered vascular function after ovarian hormonal withdrawal and the changes in bone turnover associated with osteoporosis. © 2001 Orthopaedic Research Society. Published by Elsevier Science Ltd. All rights reserved.
References
- 1 Bartelink ML, Wollersheim H, Theeuwes A, van-Duren D, Thien T. Changes in skin blood flow during the menstrual cycle: the influence of the menstrual cycle on the peripheral circulation in healthy female volunteers. Clin Sci 1990; 78: 527–32.
- 2 Bourne T, Hillard TC, Whitehead MI, Crook D, Campbell S. Oestrogens, arterial status, and postmenopausal women [letter]. Lancet 1990; 335: 1470–1.
- 3 Brandi ML, Crescioli C, Tanini A, Frediani U, Agnusdei D, Gennari C. Bone endothelial cells as estrogen targets. Calcif Tissue Int 1993; 53: 312–7.
- 4 Brinker MR, Lippton HL, Cook SD, Hyman AL. Pharmacological regulation of the circulation of bone. J Bone Joint Surg 1990; 72A: 964–75.
- 5 Burkhardt R, Kettner G, Bohm W, et al. Changes in trabecular bone, hematopoiesis and bone marrow vessels in aplastic anemia, primary osteoporosis, and old age: a comparative histomorphometric study. Bone 1987; 8: 157–64.
- 6 Collins P, Beale CM, Rosano GM, Chierchia SL, Leonardo F. Oestrogen as a calcium channal blocker. Cardioprotective effects of ovarian hormones. Eur Heart J 1996; 17(Suppl D): 15–9.
- 7 Collins P, Shay J, Jiang C, Moss J. Nitric oxide accounts for dose-dependent estrogen-mediated coronary relaxation after acute estrogen withdrawal. Circulation 1994; 90(14): 1964–8.
- 8 Davis TR, Wood MB. Endothelial control of long bone vascular resistance. J Orthop Res 1992; 10: 344–9.
- 9 Egrise D, Martin D, Neve P, Vienne A, Verhas M, Schoutens A. Bone blood flow and in vitro proliferation of bone marrow and trabecular bone osteoblast-like cells in ovariectomized rats. Calcif Tissue Int 1992; 50: 336–41.
- 10 Eriksen EF, Colvard DS, Berg NJ, Graham ML, Mann KG, Spelsberg TC, Riggs BL. Evidence of estrogen receptors in normal human osteoblast-like cells. Science 1988; 241: 84–6.
- 11 Eriksen EF, Langdahl BL. The pathogenesis of osteoporosis. Horm Res 1997; 48(Suppl 5): 78–82.
- 12 Eriksen EF, Steiniche T, Mosekilde L, Melsen F. Histomorphometric analysis of bone in metabolic bone disease. In: Metabolic bone disease, Part I. Endocrinology and metabolism clinics of North Amerika 1989; 18: 919–54.
- 13 Gangar KF, Vyas S, Whitehead M, Crook D, Meire H, Campbell S. Pulsatility index in internal carotid artery in relation to transdermal oestradiol and time since menopause. Lancet 1991; 338: 839–42.
- 14 Gilligan DM, Badar DM, Panza JA, Quyyumi AA, Cannon RO, Effects of estrogen replacement teraphy on peripheral vasomotor function on postmenopausal women. Am J Cardiol 1995; 75: 264–68.
- 15 Gilligan DM, Quyyumi AA, Cannon RO. Effects of physiological levels of estrogen on coronary vasomotor function in postmenopausal women. Circulation 1994; 89: 2545–51.
- 16
Hansen VB,
Skajaa K,
Aalkær C,
Glavind EB,
Petersen OB,
Forman A.
The effect of oophorectomy on mechanical properties of rabbit isolated cerebral and coronary resistance arteries.
Am J Obstet Gynecol
1996;
175(5):
1272–80.
10.1016/S0002-9378(96)70040-6 Google Scholar
- 17 Hayashi T, Fukuto JM, Ignarro LJ, Chaudhuri G. Basal release of nitric oxide from aortic rings is greater in female rabbits than in male rabbits: implications for atherosclerosis. Proc Natl Acad Sci USA 1992; 89: 11259–63.
- 18 Hayashi T, Yamada K, Esaki T, Kuzuya M, Satake S, Ishikawa T, et al. Estrogen increases nitric oxide by receptor-mediated system. Biochem Biophys Res Commun 1995; 214(3): 847–55.
- 19 Hillard TC, Bourne TH, Whitehead MI, Crayford TB, Collins WP, Campbell S. Differential effects of transdermal estradiol and sequential progestogens on impedance to flow within the uterine arteries of postmenopausal women. Fertil Steril 1992; 58: 959–63.
- 20 Jilka RL, Hangoc G, Girasole G, et al. Increased osteoclast development after estrogen loss: mediation by interleukin-6. Science 1992; 257: 88–91.
- 21 Kapitola J, Andrle J, Kubickova J. Possible participation of prostaglandins in the increase in the bone blood flow in oopho-rectomized female rats. Exp Clin Endocrinol 1994; 102: 414–6.
- 22 Kirkeby OJ, Berg Larsen T. Regional blood flow and strontium-85 incorporation rate in the rat hindlimb skeleton. J Orthop Res 1991; 9: 862–8.
- 23
Lindsay R.
Osteoporosis.
Clin Geriatr Med
1988;
4: 411–30.
10.1016/S0749-0690(18)30756-0 Google Scholar
- 24 Lundgaard A, Aalkjaer C, Holm Nielsen P, Mulvany MJ, Hansen ES. Method for assessment of vascular reactivity in bone: in vitro studies on resistance arteries isolated from porcine cancellous bone. J Orthop Res 1996; 14: 962–71.
- 25 Magness RR, Rosenfeld CR. Local and systemic estradiol-17 beta effects on uterine and systemic vasodilation. Am J Physiol 1989; 256: E536–42.
- 26 McCarthy ID, Lang Y. The realtionship between bone blood flow, strontium clerance, and bone turnover in tibial cortex. Trans Orthop Res Soc 1992; 17: 11–4.
- 27 Miller VM, Vanhoutte PM. 17 beta-Estradiol augments endothelium dependent contractions to arachidonic acid in rabbit aorta. Am J Physiol 1990; 258: R502–E507.
- 28 Mulvany MJ, Halpern W. Contractile properties of small arterial resistance vessels in spontaneously hypertensive and normotensive rats. Circ Res 1977; 41: 19–26.
- 29 Penotti M, Nencioni T, Gabrielli L, Farina M, Castiglioni E, Polvani F. Blood flow variations in internal carotid and middle cerebral arteries induced by postmenopausal hormone replacement therapy. Am J Obstet Gynecol 1993; 169: 1226–32.
- 30 Pensler JM, Radosevich JA, Higbee R, Langman CB. Osteoclasts isolated from membranous bone in children exhibit nuclear estrogen and progesterone receptors. J Bone Miner Res 1990; 5: 797–802.
- 31 Pirhonen JP, Vuento MH, Makinen JI, Salmi TA. Long-term effects of hormone replacement therapy on the uterus and on uterine circulation. Am J Obstet Gynecol 1993; 168: 620–30.
- 32 Reis SE, Gloth ST, Blumenthal RS, et al. Ethinyl estradiol acutely attenuates abnormal coronary vasomotor responses to acetylcholine in postmenopausal women. Circulation 1994; 89: 52–60.
- 33 Roorda J, Han CS, Wood MB. In vivo confirmation of bone blood flow control by endothelial derived relaxation factor in the canine tibia. Trans Orthop Res Soc 1992; 17: 8.
- 34 Rosselli M, Imthurn B, Keller PJ, Jackson EK, Dubey RK. Circulating nitric oxide (nitrite/nitrate) levels in prostmenopausal women substituted with 17 beta-estradiol nd norethisterone acetate. A two-year follow-up study. Hypertension 1996; 25(4 Pt 2): 848–53.
