Topical S-nitrosoglutathione-releasing hydrogel improves healing of rat ischaemic wounds
J. L. Georgii
Histology and Embryology Department, State University of Rio de Janeiro, UERJ, Rio de Janeiro, Brazil
Search for more papers by this authorT. P. Amadeu
Histology and Embryology Department, State University of Rio de Janeiro, UERJ, Rio de Janeiro, Brazil
Leprosy Laboratory, Oswald Cruz Institute, FIOCRUZ, Rio de Janeiro, Brazil
Search for more papers by this authorA. B. Seabra
Institute of Chemistry, University of Campinas, UNICAMP, Campinas, São Paulo, Brazil
Search for more papers by this authorM. G. de Oliveira
Institute of Chemistry, University of Campinas, UNICAMP, Campinas, São Paulo, Brazil
Search for more papers by this authorCorresponding Author
A. Monte-Alto-Costa
Histology and Embryology Department, State University of Rio de Janeiro, UERJ, Rio de Janeiro, Brazil
State University of Rio de Janeiro (UERJ), Histology and Embryology Department, Rua Professor Manoel de Abreu 444, 30 Andar, 20550-170, Rio de Janeiro, RJ, Brazil.Search for more papers by this authorJ. L. Georgii
Histology and Embryology Department, State University of Rio de Janeiro, UERJ, Rio de Janeiro, Brazil
Search for more papers by this authorT. P. Amadeu
Histology and Embryology Department, State University of Rio de Janeiro, UERJ, Rio de Janeiro, Brazil
Leprosy Laboratory, Oswald Cruz Institute, FIOCRUZ, Rio de Janeiro, Brazil
Search for more papers by this authorA. B. Seabra
Institute of Chemistry, University of Campinas, UNICAMP, Campinas, São Paulo, Brazil
Search for more papers by this authorM. G. de Oliveira
Institute of Chemistry, University of Campinas, UNICAMP, Campinas, São Paulo, Brazil
Search for more papers by this authorCorresponding Author
A. Monte-Alto-Costa
Histology and Embryology Department, State University of Rio de Janeiro, UERJ, Rio de Janeiro, Brazil
State University of Rio de Janeiro (UERJ), Histology and Embryology Department, Rua Professor Manoel de Abreu 444, 30 Andar, 20550-170, Rio de Janeiro, RJ, Brazil.Search for more papers by this authorAbstract
Topical application of the nitric oxide (NO) donor S-nitrosoglutathione (GSNO) is known to exert beneficial effects on wound healing. The aim of this study was to evaluate, for the first time, the effect of topical application of GSNO on the healing of ischaemic wounds. Wistar rats were submitted to two parallels incisions on their backs; the skin was separated from the underlying tissue, the incisions were sutured and an excisional wound was made between the parallel incisions to create an ischaemic condition surrounding the wound. The animals were separated into a control group, which received a hydrogel vehicle without GSNO, and a GSNO-treated group, which received a GSNO-containing hydrogel. The animals were treated for 7 days consecutively with one daily application. The GSNO-treated group displayed higher rates of wound contraction and re-epithelization, lower amounts of inflammatory cells, an increase in collagen fibre density and organization and a decrease in the neovascularization compared to control. These results show that topical application of GSNO is effective in the treatment of ischaemic wounds, leading to a significant improvement in the wound healing. Therefore, topical GSNO-containing hydrogels have potential for the therapeutic treatment of ischaemic diabetic and venous ulcers. Copyright © 2010 John Wiley & Sons, Ltd.
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