Plasma polymer surface modified expanded polytetrafluoroethylene promotes epithelial monolayer formation in vitro and can be transplanted into the dystrophic rat subretinal space
Shen Nian
Department of Ophthalmology, Li Ka Shing Faculty of Medicine, the University of Hong Kong, Hong Kong, China
Department of Eye and Vision Science, Institute of Life Course and Medical Sciences, University of Liverpool, Liverpool, UK
Search for more papers by this authorCorresponding Author
Victoria R. Kearns
Department of Eye and Vision Science, Institute of Life Course and Medical Sciences, University of Liverpool, Liverpool, UK
Correspondence
Victoria Kearns, Department of Eye and Vision Science, Institute of Life Course and Medical Sciences, University of Liverpool, William Henry Duncan Building, West Derby Street, Liverpool, L7 8TX, UK.
Email: [email protected]
Present address
Shen Nilan, Department of Pathology, Xi'an Medical University, Xi'an, Shaanxi Province, 710021, China.
Search for more papers by this authorDavid S. H. Wong
Department of Ophthalmology, Li Ka Shing Faculty of Medicine, the University of Hong Kong, Hong Kong, China
Department of Eye and Vision Science, Institute of Life Course and Medical Sciences, University of Liverpool, Liverpool, UK
Search for more papers by this authorAkash Bachhuka
School of Engineering, University of South Australia, Adelaide, South Australia, Australia
Search for more papers by this authorKrasimir Vasilev
School of Engineering, University of South Australia, Adelaide, South Australia, Australia
Search for more papers by this authorRachel L. Williams
Department of Eye and Vision Science, Institute of Life Course and Medical Sciences, University of Liverpool, Liverpool, UK
Search for more papers by this authorWico W. Lai
Department of Ophthalmology, Li Ka Shing Faculty of Medicine, the University of Hong Kong, Hong Kong, China
Search for more papers by this authorAmy Lo
Department of Ophthalmology, Li Ka Shing Faculty of Medicine, the University of Hong Kong, Hong Kong, China
Search for more papers by this authorCarl M. Sheridan
Department of Eye and Vision Science, Institute of Life Course and Medical Sciences, University of Liverpool, Liverpool, UK
Search for more papers by this authorShen Nian
Department of Ophthalmology, Li Ka Shing Faculty of Medicine, the University of Hong Kong, Hong Kong, China
Department of Eye and Vision Science, Institute of Life Course and Medical Sciences, University of Liverpool, Liverpool, UK
Search for more papers by this authorCorresponding Author
Victoria R. Kearns
Department of Eye and Vision Science, Institute of Life Course and Medical Sciences, University of Liverpool, Liverpool, UK
Correspondence
Victoria Kearns, Department of Eye and Vision Science, Institute of Life Course and Medical Sciences, University of Liverpool, William Henry Duncan Building, West Derby Street, Liverpool, L7 8TX, UK.
Email: [email protected]
Present address
Shen Nilan, Department of Pathology, Xi'an Medical University, Xi'an, Shaanxi Province, 710021, China.
Search for more papers by this authorDavid S. H. Wong
Department of Ophthalmology, Li Ka Shing Faculty of Medicine, the University of Hong Kong, Hong Kong, China
Department of Eye and Vision Science, Institute of Life Course and Medical Sciences, University of Liverpool, Liverpool, UK
Search for more papers by this authorAkash Bachhuka
School of Engineering, University of South Australia, Adelaide, South Australia, Australia
Search for more papers by this authorKrasimir Vasilev
School of Engineering, University of South Australia, Adelaide, South Australia, Australia
Search for more papers by this authorRachel L. Williams
Department of Eye and Vision Science, Institute of Life Course and Medical Sciences, University of Liverpool, Liverpool, UK
Search for more papers by this authorWico W. Lai
Department of Ophthalmology, Li Ka Shing Faculty of Medicine, the University of Hong Kong, Hong Kong, China
Search for more papers by this authorAmy Lo
Department of Ophthalmology, Li Ka Shing Faculty of Medicine, the University of Hong Kong, Hong Kong, China
Search for more papers by this authorCarl M. Sheridan
Department of Eye and Vision Science, Institute of Life Course and Medical Sciences, University of Liverpool, Liverpool, UK
Search for more papers by this authorAbstract
The aim of this study was to evaluate whether the surface modification of expanded polytetrafluoroethylene (ePTFE) using an n-heptylamine (HA) plasma polymer would allow for functional epithelial monolayer formation suitable for subretinal transplant into a non-dystrophic rat model. Freshly isolated iris pigment epithelial (IPE) cells from two rat strains (Long Evans [LE] and Dark Agouti [DA]) were seeded onto HA, fibronectin-coated n-heptylamine modified (F-HA) and unmodified ePFTE and fibronectin-coated tissue culture (F-TCPS) substrates. Both F-HA ePTFE and F-TCPS substrates enabled functional monolayer formation with both strains of rat. Without fibronectin coating, only LE IPE formed a monolayer on HA-treated ePTFE. Functional assessment of both IPE strains on F-HA ePTFE demonstrated uptake of POS that increased significantly with time that was greater than control F-TCPS. Surgical optimization using Healon GV and mixtures of Healon GV: phosphate buffered saline (PBS) to induce retinal detachment demonstrated that only Healon GV:PBS allowed F-HA ePTFE substrates to be successfully transplanted into the subretinal space of Royal College of Surgeons rats, where they remained flat beneath the neural retina for up to 4 weeks. No apparent substrate-induced inflammatory response was observed by fundus microscopy or immunohistochemical analysis, indicating the potential of this substrate for future clinical applications.
CONFLICT OF INTEREST STATEMENT
The authors have no conflict of interest.
Open Research
DATA AVAILABILITY STATEMENT
The datasets generated and/or analyzed during the current study will be made available in University of Liverpool open access data repository.
Supporting Information
| Filename | Description |
|---|---|
| term3154-sup-0001-fig_s1.tif929.6 KB | SupplementaryInformation1 |
| term3154-sup-0002-fig_s2.tif1.5 MB | SupplementaryInformation2 |
Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.
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