Intratendon delivery of leukocyte-rich platelet-rich plasma at early stage promotes tendon repair in a rabbit Achilles tendinopathy model
Correction(s) for this article
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Corrigendum
- Volume 16Issue 1Journal of Tissue Engineering and Regenerative Medicine
- pages: 86-87
- First Published online: January 6, 2022
Sihao Li
Department of Orthopedic Surgery, 2nd Affiliated Hospital, School of Medicine, Zhejiang University, Zhejiang, China
Search for more papers by this authorYifan Wu
Department of Surgery, Zhejiang University Hospital, Zhejiang University, Hangzhou, China
Search for more papers by this authorGuangyao Jiang
Department of Orthopedic Surgery, 2nd Affiliated Hospital, School of Medicine, Zhejiang University, Zhejiang, China
Search for more papers by this authorXiulian Tian
Department of Neurology, 2nd Affiliated Hospital, School of Medicine, Zhejiang University, Zhejiang, China
Search for more papers by this authorJianqiao Hong
Department of Orthopedic Surgery, 2nd Affiliated Hospital, School of Medicine, Zhejiang University, Zhejiang, China
Search for more papers by this authorShiming Chen
Department of Surgery, Shaoxing Second Hospital, Shaoxing, China
Search for more papers by this authorCorresponding Author
Ruijian Yan
Department of Orthopedic Surgery, 2nd Affiliated Hospital, School of Medicine, Zhejiang University, Zhejiang, China
Correspondence
Ruijian Yan and Gang Feng, Department of Orthopedic Surgery, Second Affiliated Hospital, Zhejiang University School of Medicine, 88 Jie Fang Road, Hangzhou 310009, China.
Email: [email protected]; [email protected]
Search for more papers by this authorCorresponding Author
Gang Feng
Department of Orthopedic Surgery, 2nd Affiliated Hospital, School of Medicine, Zhejiang University, Zhejiang, China
Correspondence
Ruijian Yan and Gang Feng, Department of Orthopedic Surgery, Second Affiliated Hospital, Zhejiang University School of Medicine, 88 Jie Fang Road, Hangzhou 310009, China.
Email: [email protected]; [email protected]
Search for more papers by this authorZhiyuan Cheng
Institute of Microelectronics and Nanoelectronics, Key Lab. of Advanced Micro/Nano Electronics Devices & Smart Systems of Zhejiang, College of Information Science & Electronic Engineering, Zhejiang University, Hangzhou, China
Search for more papers by this authorSihao Li
Department of Orthopedic Surgery, 2nd Affiliated Hospital, School of Medicine, Zhejiang University, Zhejiang, China
Search for more papers by this authorYifan Wu
Department of Surgery, Zhejiang University Hospital, Zhejiang University, Hangzhou, China
Search for more papers by this authorGuangyao Jiang
Department of Orthopedic Surgery, 2nd Affiliated Hospital, School of Medicine, Zhejiang University, Zhejiang, China
Search for more papers by this authorXiulian Tian
Department of Neurology, 2nd Affiliated Hospital, School of Medicine, Zhejiang University, Zhejiang, China
Search for more papers by this authorJianqiao Hong
Department of Orthopedic Surgery, 2nd Affiliated Hospital, School of Medicine, Zhejiang University, Zhejiang, China
Search for more papers by this authorShiming Chen
Department of Surgery, Shaoxing Second Hospital, Shaoxing, China
Search for more papers by this authorCorresponding Author
Ruijian Yan
Department of Orthopedic Surgery, 2nd Affiliated Hospital, School of Medicine, Zhejiang University, Zhejiang, China
Correspondence
Ruijian Yan and Gang Feng, Department of Orthopedic Surgery, Second Affiliated Hospital, Zhejiang University School of Medicine, 88 Jie Fang Road, Hangzhou 310009, China.
Email: [email protected]; [email protected]
Search for more papers by this authorCorresponding Author
Gang Feng
Department of Orthopedic Surgery, 2nd Affiliated Hospital, School of Medicine, Zhejiang University, Zhejiang, China
Correspondence
Ruijian Yan and Gang Feng, Department of Orthopedic Surgery, Second Affiliated Hospital, Zhejiang University School of Medicine, 88 Jie Fang Road, Hangzhou 310009, China.
Email: [email protected]; [email protected]
Search for more papers by this authorZhiyuan Cheng
Institute of Microelectronics and Nanoelectronics, Key Lab. of Advanced Micro/Nano Electronics Devices & Smart Systems of Zhejiang, College of Information Science & Electronic Engineering, Zhejiang University, Hangzhou, China
Search for more papers by this authorAbstract
Tendinopathy is a great obstacle in clinical practice due to its poor regenerative capacity. The influence of different stages of tendinopathy on effects of leukocyte-rich platelet-rich plasma (Lr-PRP) has not been elucidated. The aim of this study is to investigate the optimal time point for delivery of Lr-PRP on tendinopathy. A tendinopathy model was established by local collagenase injection on the rabbit Achilles tendon. Then after collagenase induction, following treatments were applied randomly on the lesion: (a) 200 μl of Lr-PRP at 1 week (PRP-1 group), (b) 200 μl of saline at 1 week (Saline-1 group), (c) 200 μl of Lr-PRP at 4 weeks (PRP-2 group), and (d) 200 μl of saline at 4 weeks (Saline-2 group). Six weeks after collagenase induction, outcomes were assessed by magnetic resonance imaging, cytokine quantification, gene expression, histology, and transmission electron microscopy. Our results demonstrated that PRP-1 group had the least cross-sectional area and lesion percent of the involved tendon, as well as the lowest signal intensity in magnetic resonance imaging among all groups. However, the PRP-2 group showed larger cross-sectional area than saline groups. Enzyme-linked immunosorbent assay indicated that PRP-1 group had a higher level of interleukin-10 but lower level of interleukin-6 when compared with PRP-2 and saline groups. Meanwhile, the highest expression of collagen (Col) 1 in PRP-1 and Col 3, matrix metalloproteinase (MMP)-1, and MMP-3 in PRP-2 was found. Histologically, the PRP-1 showed better general scores than PRP-2, and no significant difference was found between the PRP-2 and saline groups. For transmission electron microscopy, PRP-1 had the largest mean collagen fibril diameter, and the PRP-2 group showed even smaller mean collagen fibril diameter than saline groups. In conclusion, intratendon delivery of Lr-PRP at early stage showed beneficial effect for repair of tendinopathy but not at late stage. For translation of our results to clinical circumstances, further studies are still needed.
CONFLICT OF INTEREST
The authors declare there are no financial conflicts of interest.
Supporting Information
| Filename | Description |
|---|---|
| term3006-sup-0001_Figures.docxWord 2007 document , 4.7 MB |
Figure S1. Immunohistochemistry images of CD163 marked M2 macrophage in the (A) Normal, (B) Saline-1, (C) PRP-1, (D) Saline-2 and (E) PRP-2 group. Scale bar: 200μm. Figure S2.The Achilles tendon was firmly fixed on the Instron machine to perform the mechanical test Figure S3. The tendon was removed after reaching maximum load. The arrow line indicated the small cracks. Figure S4.Histology evaluation. (A) Fiber arrangement score. (B) Fiber structure score. (C) Angiogenesis score. (D)Rounding of nuclear score.(E) Inflammation score. (F) Cell density score.*P<.05. Figure S5.Distribution of collagen fibril diameters in the Saline-1 (A), PRP-1 (B), Saline-2(C), PRP-2 (D)and Normal (E) groups. |
| term3006-sup-0002_Tables.docxWord 2007 document , 17.7 KB |
Table S1. Mechanical properties of tendon repair. Table S2. Histological evaluation scores of tendon. |
Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.
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