A clinical-grade acellular matrix for esophageal replacement
Lousineh Arakelian
Unité de Thérapie Cellulaire, Hôpital Saint-Louis, AP-HP, Paris, France
Institut de Recherche Saint Louis, INSERM, CIC-BT1427 and UMR-U976, Hôpital St-Louis, Paris, France
Search for more papers by this authorClémentine Caille
Unité de Thérapie Cellulaire, Hôpital Saint-Louis, AP-HP, Paris, France
Institut de Recherche Saint Louis, INSERM, CIC-BT1427 and UMR-U976, Hôpital St-Louis, Paris, France
Search for more papers by this authorLionel Faivre
Unité de Thérapie Cellulaire, Hôpital Saint-Louis, AP-HP, Paris, France
Institut de Recherche Saint Louis, INSERM, CIC-BT1427 and UMR-U976, Hôpital St-Louis, Paris, France
Search for more papers by this authorLaurent Corté
MAT—Centre des Matériaux, MINES ParisTech, PSL Research University, CNRS UMR 7633, France
Laboratoire Matière Molle et Chimie, ESPCI Paris, PSL Research University, CNRS UMR 7167, Paris, France
Search for more papers by this authorPatrick Bruneval
Department of Pathology, Georges Pompidou European Hospital, AP-HP, Paris, France
Search for more papers by this authorSara Shamdani
Laboratoire CRRET, Université Paris Est Créteil, Université Paris Est, EA 4397 ERL CNRS 9215, Créteil, France
Search for more papers by this authorCamille Flageollet
Laboratoire CRRET, Université Paris Est Créteil, Université Paris Est, EA 4397 ERL CNRS 9215, Créteil, France
Search for more papers by this authorPatricia Albanese
Laboratoire CRRET, Université Paris Est Créteil, Université Paris Est, EA 4397 ERL CNRS 9215, Créteil, France
Search for more papers by this authorThomas Domet
Unité de Thérapie Cellulaire, Hôpital Saint-Louis, AP-HP, Paris, France
Institut de Recherche Saint Louis, INSERM, CIC-BT1427 and UMR-U976, Hôpital St-Louis, Paris, France
Search for more papers by this authorMohamed Jarraya
Banque des Tissus Humains, Hôpital St-Louis, AP-HP, Paris, France
Search for more papers by this authorNiclas Setterblad
Technological Core facility of the Hematology Institute, Université Paris-Diderot and Inserm, Hôpital Saint-Louis, Paris, France
Search for more papers by this authorSabrina Kellouche
Equipe de Recherche sur les Relations Matrice Extracellulaire-Cellules, ERRMECe (EA1391), Institut des Matériaux, I-MAT (FD4122), University of Cergy-Pontoise, MIR, France
Search for more papers by this authorJérôme Larghero
Unité de Thérapie Cellulaire, Hôpital Saint-Louis, AP-HP, Paris, France
Institut de Recherche Saint Louis, INSERM, CIC-BT1427 and UMR-U976, Hôpital St-Louis, Paris, France
Search for more papers by this authorCorresponding Author
Pierre Cattan
Institut de Recherche Saint Louis, INSERM, CIC-BT1427 and UMR-U976, Hôpital St-Louis, Paris, France
Department of Digestive Surgery, St-Louis Hospital—Paris 7 University, Paris, France
Correspondence
Valérie Vanneaux, Unité de Thérapie Cellulaire, Hôpital Saint-Louis, AP-HP. Paris, France.
Email: [email protected]
Pierre Cattan, Institut de Recherche Saint Louis, INSERM, CIC-BT1427 and UMR-U976, Hôpital St-Louis. Paris, France.
Email: [email protected]
Search for more papers by this authorCorresponding Author
Valérie Vanneaux
Unité de Thérapie Cellulaire, Hôpital Saint-Louis, AP-HP, Paris, France
Institut de Recherche Saint Louis, INSERM, CIC-BT1427 and UMR-U976, Hôpital St-Louis, Paris, France
Correspondence
Valérie Vanneaux, Unité de Thérapie Cellulaire, Hôpital Saint-Louis, AP-HP. Paris, France.
Email: [email protected]
Pierre Cattan, Institut de Recherche Saint Louis, INSERM, CIC-BT1427 and UMR-U976, Hôpital St-Louis. Paris, France.
Email: [email protected]
Search for more papers by this authorLousineh Arakelian
Unité de Thérapie Cellulaire, Hôpital Saint-Louis, AP-HP, Paris, France
Institut de Recherche Saint Louis, INSERM, CIC-BT1427 and UMR-U976, Hôpital St-Louis, Paris, France
Search for more papers by this authorClémentine Caille
Unité de Thérapie Cellulaire, Hôpital Saint-Louis, AP-HP, Paris, France
Institut de Recherche Saint Louis, INSERM, CIC-BT1427 and UMR-U976, Hôpital St-Louis, Paris, France
Search for more papers by this authorLionel Faivre
Unité de Thérapie Cellulaire, Hôpital Saint-Louis, AP-HP, Paris, France
Institut de Recherche Saint Louis, INSERM, CIC-BT1427 and UMR-U976, Hôpital St-Louis, Paris, France
Search for more papers by this authorLaurent Corté
MAT—Centre des Matériaux, MINES ParisTech, PSL Research University, CNRS UMR 7633, France
Laboratoire Matière Molle et Chimie, ESPCI Paris, PSL Research University, CNRS UMR 7167, Paris, France
Search for more papers by this authorPatrick Bruneval
Department of Pathology, Georges Pompidou European Hospital, AP-HP, Paris, France
Search for more papers by this authorSara Shamdani
Laboratoire CRRET, Université Paris Est Créteil, Université Paris Est, EA 4397 ERL CNRS 9215, Créteil, France
Search for more papers by this authorCamille Flageollet
Laboratoire CRRET, Université Paris Est Créteil, Université Paris Est, EA 4397 ERL CNRS 9215, Créteil, France
Search for more papers by this authorPatricia Albanese
Laboratoire CRRET, Université Paris Est Créteil, Université Paris Est, EA 4397 ERL CNRS 9215, Créteil, France
Search for more papers by this authorThomas Domet
Unité de Thérapie Cellulaire, Hôpital Saint-Louis, AP-HP, Paris, France
Institut de Recherche Saint Louis, INSERM, CIC-BT1427 and UMR-U976, Hôpital St-Louis, Paris, France
Search for more papers by this authorMohamed Jarraya
Banque des Tissus Humains, Hôpital St-Louis, AP-HP, Paris, France
Search for more papers by this authorNiclas Setterblad
Technological Core facility of the Hematology Institute, Université Paris-Diderot and Inserm, Hôpital Saint-Louis, Paris, France
Search for more papers by this authorSabrina Kellouche
Equipe de Recherche sur les Relations Matrice Extracellulaire-Cellules, ERRMECe (EA1391), Institut des Matériaux, I-MAT (FD4122), University of Cergy-Pontoise, MIR, France
Search for more papers by this authorJérôme Larghero
Unité de Thérapie Cellulaire, Hôpital Saint-Louis, AP-HP, Paris, France
Institut de Recherche Saint Louis, INSERM, CIC-BT1427 and UMR-U976, Hôpital St-Louis, Paris, France
Search for more papers by this authorCorresponding Author
Pierre Cattan
Institut de Recherche Saint Louis, INSERM, CIC-BT1427 and UMR-U976, Hôpital St-Louis, Paris, France
Department of Digestive Surgery, St-Louis Hospital—Paris 7 University, Paris, France
Correspondence
Valérie Vanneaux, Unité de Thérapie Cellulaire, Hôpital Saint-Louis, AP-HP. Paris, France.
Email: [email protected]
Pierre Cattan, Institut de Recherche Saint Louis, INSERM, CIC-BT1427 and UMR-U976, Hôpital St-Louis. Paris, France.
Email: [email protected]
Search for more papers by this authorCorresponding Author
Valérie Vanneaux
Unité de Thérapie Cellulaire, Hôpital Saint-Louis, AP-HP, Paris, France
Institut de Recherche Saint Louis, INSERM, CIC-BT1427 and UMR-U976, Hôpital St-Louis, Paris, France
Correspondence
Valérie Vanneaux, Unité de Thérapie Cellulaire, Hôpital Saint-Louis, AP-HP. Paris, France.
Email: [email protected]
Pierre Cattan, Institut de Recherche Saint Louis, INSERM, CIC-BT1427 and UMR-U976, Hôpital St-Louis. Paris, France.
Email: [email protected]
Search for more papers by this authorAbstract
In pathologies of the esophagus such as esophageal atresia, cancers, and caustic injuries, methods for full thickness esophageal replacement require the sacrifice of healthy intra-abdominal organs such as the stomach and the colon and are associated with high morbidity, mortality, and poor functional results. To overcome these problems, tissue engineering methods are developed to create a substitute with scaffolds and cells. The aim of this study was to develop a simple and safe decellularization process in order to obtain a clinical grade esophageal extracellular matrix. Following the decontamination step, porcine esophagi were decellularized in a bioreactor with sodium dodecyl sulfate and ethylenediaminetetraacetic acid for 3 days and were rinsed with deionized water. DNA was eliminated by a 3-hr DNase treatment. To remove any residual detergent, the matrix was then incubated with an absorbing resin. The resulting porcine esophageal matrix was characterized by the assessment of the efficiency of the decellularization process (DNA quantification), evaluation of sterility and absence of cytotoxicity, and its composition and biomechanical properties, as well as the possibility to be reseeded with mesenchymal stem cells. Complete decellularization with the preservation of the general structure, composition, and biomechanical properties of the native esophageal matrix was obtained. Sterility was maintained throughout the process, and the matrix showed no cytotoxicity. The resulting matrix met clinical grade criteria and was successfully reseeded with mesenchymal stem cells..
CONFLICT OF INTEREST
The authors declare that they have no conflict of interest.
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