MicroRNA-132 directs human periodontal ligament-derived neural crest stem cell neural differentiation
Tsz Kin Ng
Joint Shantou International Eye Center of Shantou University and The Chinese University of Hong Kong, Shantou, Guangdong, China
Shantou University Medical College, Shantou, Guangdong, China
Department of Ophthalmology and Visual Sciences, The Chinese University of Hong Kong, Kowloon, Hong Kong
Geriatric Research, Education and Clinical Center, Miami Veterans Affairs Medical Center, Miami, Florida
These authors contributed equally to this work.Search for more papers by this authorQichen Yang
Department of Ophthalmology and Visual Sciences, The Chinese University of Hong Kong, Kowloon, Hong Kong
These authors contributed equally to this work.Search for more papers by this authorVeronica R. Fortino
Department of Biomedical Engineering, College of Engineering, University of Miami, Coral Gables, Florida
Department of Biological Sciences, Nova Southeastern University, Fort Lauderdale, Florida
Search for more papers by this authorNikky Yuk-Ki Lai
Department of Ophthalmology and Visual Sciences, The Chinese University of Hong Kong, Kowloon, Hong Kong
Search for more papers by this authorCarlos M. Carballosa
Department of Biomedical Engineering, College of Engineering, University of Miami, Coral Gables, Florida
Search for more papers by this authorJordan M. Greenberg
Department of Biomedical Engineering, College of Engineering, University of Miami, Coral Gables, Florida
Search for more papers by this authorKwong Wai Choy
Department of Obstetrics and Gynaecology, The Chinese University of Hong Kong, Shatin, Hong Kong
Search for more papers by this authorDaniel Pelaez
Geriatric Research, Education and Clinical Center, Miami Veterans Affairs Medical Center, Miami, Florida
Department of Ophthalmology, Bascom Palmer Eye Institute, University of Miami, Miami, Florida
Search for more papers by this authorChi Pui Pang
Joint Shantou International Eye Center of Shantou University and The Chinese University of Hong Kong, Shantou, Guangdong, China
Department of Ophthalmology and Visual Sciences, The Chinese University of Hong Kong, Kowloon, Hong Kong
Search for more papers by this authorCorresponding Author
Herman S. Cheung
Geriatric Research, Education and Clinical Center, Miami Veterans Affairs Medical Center, Miami, Florida
Department of Biomedical Engineering, College of Engineering, University of Miami, Coral Gables, Florida
Correspondence
Herman S. Cheung, Geriatric Research, Education and Clinical Center, Miami Veterans Affairs Medical Center, 1201 NW 16th Street, Miami, FL 33125.
Email: [email protected]
Search for more papers by this authorTsz Kin Ng
Joint Shantou International Eye Center of Shantou University and The Chinese University of Hong Kong, Shantou, Guangdong, China
Shantou University Medical College, Shantou, Guangdong, China
Department of Ophthalmology and Visual Sciences, The Chinese University of Hong Kong, Kowloon, Hong Kong
Geriatric Research, Education and Clinical Center, Miami Veterans Affairs Medical Center, Miami, Florida
These authors contributed equally to this work.Search for more papers by this authorQichen Yang
Department of Ophthalmology and Visual Sciences, The Chinese University of Hong Kong, Kowloon, Hong Kong
These authors contributed equally to this work.Search for more papers by this authorVeronica R. Fortino
Department of Biomedical Engineering, College of Engineering, University of Miami, Coral Gables, Florida
Department of Biological Sciences, Nova Southeastern University, Fort Lauderdale, Florida
Search for more papers by this authorNikky Yuk-Ki Lai
Department of Ophthalmology and Visual Sciences, The Chinese University of Hong Kong, Kowloon, Hong Kong
Search for more papers by this authorCarlos M. Carballosa
Department of Biomedical Engineering, College of Engineering, University of Miami, Coral Gables, Florida
Search for more papers by this authorJordan M. Greenberg
Department of Biomedical Engineering, College of Engineering, University of Miami, Coral Gables, Florida
Search for more papers by this authorKwong Wai Choy
Department of Obstetrics and Gynaecology, The Chinese University of Hong Kong, Shatin, Hong Kong
Search for more papers by this authorDaniel Pelaez
Geriatric Research, Education and Clinical Center, Miami Veterans Affairs Medical Center, Miami, Florida
Department of Ophthalmology, Bascom Palmer Eye Institute, University of Miami, Miami, Florida
Search for more papers by this authorChi Pui Pang
Joint Shantou International Eye Center of Shantou University and The Chinese University of Hong Kong, Shantou, Guangdong, China
Department of Ophthalmology and Visual Sciences, The Chinese University of Hong Kong, Kowloon, Hong Kong
Search for more papers by this authorCorresponding Author
Herman S. Cheung
Geriatric Research, Education and Clinical Center, Miami Veterans Affairs Medical Center, Miami, Florida
Department of Biomedical Engineering, College of Engineering, University of Miami, Coral Gables, Florida
Correspondence
Herman S. Cheung, Geriatric Research, Education and Clinical Center, Miami Veterans Affairs Medical Center, 1201 NW 16th Street, Miami, FL 33125.
Email: [email protected]
Search for more papers by this authorAbstract
Neurogenesis is the basis of stem cell tissue engineering and regenerative medicine for central nervous system (CNS) disorders. We have established differentiation protocols to direct human periodontal ligament-derived stem cells (PDLSCs) into neuronal lineage, and we recently isolated the neural crest subpopulation from PDLSCs, which are pluripotent in nature. Here, we report the neural differentiation potential of these periodontal ligament-derived neural crest stem cells (NCSCs) as well as its microRNA (miRNA) regulatory mechanism and function in NCSC neural differentiation. NCSCs, treated with basic fibroblast growth factor and epidermal growth factor-based differentiation medium for 24 days, expressed neuronal and glial markers (βIII-tubulin, neurofilament, NeuN, neuron-specific enolase, GFAP, and S100) and exhibited glutamate-induced calcium responses. The global miRNA expression profiling identified 60 upregulated and 19 downregulated human miRNAs after neural differentiation, and the gene ontology analysis of the miRNA target genes confirmed the neuronal differentiation-related biological functions. In addition, overexpression of miR-132 in NCSCs promoted the expression of neuronal markers and downregulated ZEB2 protein expression. Our results suggested that the pluripotent NCSCs from human periodontal ligament can be directed into neural lineage, which demonstrate its potential in tissue engineering and regenerative medicine for CNS disorders.
CONFLICTS OF INTEREST
The authors disclose no conflicts of interest.
Supporting Information
| Filename | Description |
|---|---|
| term2759-sup-0001-sf1.tifTIFF image, 2.4 MB |
Figure S1: Gene enrichment analysis of neural-induced microRNA target genes. FunRich analysis of 1939 neural-induced miRNA target genes. (A) The 12 significant biological process (p < 0.05). (B) Top 12 significant biological pathway (p < 0.001). (C) Top 12 significant site of expression (p < 0.001). |
| term2759-sup-0002-st1.docWord document, 46.5 KB |
Table S1: Primers for gene expression analysis. |
| term2759-sup-0003-st2.docWord document, 39.5 KB |
Table S2: Antibodies for protein expression analysis. |
Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.
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