Biomechanical Insights into the Proteomic Profiling of Cells in Response to Red Light Absorption
Antônio V. S. Silva
Departamento de Física, Universidade Federal do Ceará, Fortaleza, CE, 60440-900 Brazil
Instituto Federal do Rio Grande do Norte, Pau dos Ferros, RN, 59900-000 Brazil
Search for more papers by this authorFelipe D. Sousa
Núcleo de Biologia Experimental, Universidade de Fortaleza, Fortaleza, CE, 60811-905 Brazil
Search for more papers by this authorBrandon F. Sousa
Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Ceará, Fortaleza, CE, 60440-900 Brazil
Search for more papers by this authorWallace V. Santos
Departamento de Física, Universidade Federal do Ceará, Fortaleza, CE, 60440-900 Brazil
Search for more papers by this authorAntônio E. R. Oliveira
Núcleo de Biologia Experimental, Universidade de Fortaleza, Fortaleza, CE, 60811-905 Brazil
Search for more papers by this authorMarina D. P. Lobo
Departamento de Biologia, Universidade Federal do Ceará, Fortaleza, CE, 60440-900 Brazil
Search for more papers by this authorMárcio V. Ramos
Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Ceará, Fortaleza, CE, 60440-900 Brazil
Search for more papers by this authorNylane M. N. Alencar
Departamento de Fisiologia e Farmacologia, Universidade Federal do Ceará, Fortaleza, CE, 60430-275 Brazil
Search for more papers by this authorMarcelo V. P. de Sousa
Departamento de Física, Universidade Federal do Ceará, Fortaleza, CE, 60440-900 Brazil
Bright Photomedicine, São Paulo, SP, 05508-000 Brazil
Instituto Federal de Sergipe, Estância, SE, 49200-000 Brazil
Search for more papers by this authorRosemayre S. Freire
Central Analítica, Universidade Federal do Ceará, Fortaleza, CE, 60440-900 Brazil
Search for more papers by this authorCláudio L. N. Oliveira
Departamento de Física, Universidade Federal do Ceará, Fortaleza, CE, 60440-900 Brazil
Search for more papers by this authorCorresponding Author
Jeanlex S. de Sousa
Departamento de Física, Universidade Federal do Ceará, Fortaleza, CE, 60440-900 Brazil
E-mail: [email protected]
Search for more papers by this authorAntônio V. S. Silva
Departamento de Física, Universidade Federal do Ceará, Fortaleza, CE, 60440-900 Brazil
Instituto Federal do Rio Grande do Norte, Pau dos Ferros, RN, 59900-000 Brazil
Search for more papers by this authorFelipe D. Sousa
Núcleo de Biologia Experimental, Universidade de Fortaleza, Fortaleza, CE, 60811-905 Brazil
Search for more papers by this authorBrandon F. Sousa
Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Ceará, Fortaleza, CE, 60440-900 Brazil
Search for more papers by this authorWallace V. Santos
Departamento de Física, Universidade Federal do Ceará, Fortaleza, CE, 60440-900 Brazil
Search for more papers by this authorAntônio E. R. Oliveira
Núcleo de Biologia Experimental, Universidade de Fortaleza, Fortaleza, CE, 60811-905 Brazil
Search for more papers by this authorMarina D. P. Lobo
Departamento de Biologia, Universidade Federal do Ceará, Fortaleza, CE, 60440-900 Brazil
Search for more papers by this authorMárcio V. Ramos
Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Ceará, Fortaleza, CE, 60440-900 Brazil
Search for more papers by this authorNylane M. N. Alencar
Departamento de Fisiologia e Farmacologia, Universidade Federal do Ceará, Fortaleza, CE, 60430-275 Brazil
Search for more papers by this authorMarcelo V. P. de Sousa
Departamento de Física, Universidade Federal do Ceará, Fortaleza, CE, 60440-900 Brazil
Bright Photomedicine, São Paulo, SP, 05508-000 Brazil
Instituto Federal de Sergipe, Estância, SE, 49200-000 Brazil
Search for more papers by this authorRosemayre S. Freire
Central Analítica, Universidade Federal do Ceará, Fortaleza, CE, 60440-900 Brazil
Search for more papers by this authorCláudio L. N. Oliveira
Departamento de Física, Universidade Federal do Ceará, Fortaleza, CE, 60440-900 Brazil
Search for more papers by this authorCorresponding Author
Jeanlex S. de Sousa
Departamento de Física, Universidade Federal do Ceará, Fortaleza, CE, 60440-900 Brazil
E-mail: [email protected]
Search for more papers by this authorAbstract
Photobiomodulation (PBM) is a promising non-invasive therapy for tissue repair, but its underlying cellular mechanisms are not fully understood. In this study, the biomechanical and proteomic responses of three cell types – keratinocytes (HACAT), fibroblasts (L929), and osteoblasts (OFCOLII) – exposed to red light (633 nm) are investigated using atomic force microscopy (AFM) and mass spectrometry-based proteomic analysis. Red light absorption resulted in cell-type-specific changes in viscoelastic properties, with fibroblasts exhibiting increased fluidity, reduced stiffness, and enhanced motility. Conversely, keratinocytes exhibited intensity-dependent responses, while osteoblasts appeared to be relatively insensitive to irradiation conditions. Proteomic profiling identified key signaling pathways involved in immune response, ATP production, and stress regulation. The immune and ATP pathways are strongly linked to the modulation of viscoelastic properties, particularly in fibroblasts, while weaker correlations were observed in keratinocytes. Cytoskeletal remodeling, primarily within the F-actin network, is identified as the main driver of mechanical alterations, with additional contributions from microtubules and intermediate filaments. These findings provide new insights into how red light absorption modulates cellular viscoelasticity through cytoskeletal remodeling, with potential applications in optimizing light-based therapies for tissue regeneration and disease treatment.
Conflict of Interest
The authors declare no conflict of interest.
Open Research
Data Availability Statement
The data that support the findings of this study are available from the corresponding author upon reasonable request.
Supporting Information
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| smll202410062-sup-0001-SuppMat.pdf2 MB | Supporting Information |
Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.
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