Development of Biodegradable Osteopromotive Citrate-Based Bone Putty
Xinyu Tan
Department of Cell Biology, School of Basic Medical Sciences, Southern Medical University, Guangzhou, Guangdong Province, 510515 China
Department of Biomedical Engineering, Materials Research Institute, The Huck Institutes of the Life Sciences, The Pennsylvania State University, University Park, PA, 16802 USA
Academy of Orthopedics, Provincial Key Laboratory of Bone and Joint Degenerative Diseases, The Third Affiliated Hospital of Southern Medical University, Guangzhou, Guangdong Province, 510280 China
Search for more papers by this authorEthan Gerhard
Department of Biomedical Engineering, Materials Research Institute, The Huck Institutes of the Life Sciences, The Pennsylvania State University, University Park, PA, 16802 USA
Search for more papers by this authorYuqi Wang
Department of Biomedical Engineering, Materials Research Institute, The Huck Institutes of the Life Sciences, The Pennsylvania State University, University Park, PA, 16802 USA
Search for more papers by this authorRichard T. Tran
Department of Biomedical Engineering, Materials Research Institute, The Huck Institutes of the Life Sciences, The Pennsylvania State University, University Park, PA, 16802 USA
Search for more papers by this authorHui Xu
Department of Biomedical Engineering, Materials Research Institute, The Huck Institutes of the Life Sciences, The Pennsylvania State University, University Park, PA, 16802 USA
Search for more papers by this authorSu Yan
Department of Biomedical Engineering, Materials Research Institute, The Huck Institutes of the Life Sciences, The Pennsylvania State University, University Park, PA, 16802 USA
Search for more papers by this authorElias B. Rizk
Department of Neurosurgery, College of Medicine, The Pennsylvania State University, Hershey, PA, 17033 USA
Search for more papers by this authorApril D. Armstrong
Department of Orthopaedics and Rehabilitation, College of Medicine, The Pennsylvania State University, Hershey, PA, 17033 USA
Search for more papers by this authorYuxiao Zhou
Department of Mechanical Engineering, The Pennsylvania State University, University Park, PA, 16802 USA
Search for more papers by this authorJing Du
Department of Mechanical Engineering, The Pennsylvania State University, University Park, PA, 16802 USA
Search for more papers by this authorCorresponding Author
Xiaochun Bai
Department of Cell Biology, School of Basic Medical Sciences, Southern Medical University, Guangzhou, Guangdong Province, 510515 China
Academy of Orthopedics, Provincial Key Laboratory of Bone and Joint Degenerative Diseases, The Third Affiliated Hospital of Southern Medical University, Guangzhou, Guangdong Province, 510280 China
E-mail: [email protected], [email protected]
Search for more papers by this authorCorresponding Author
Jian Yang
Department of Biomedical Engineering, Materials Research Institute, The Huck Institutes of the Life Sciences, The Pennsylvania State University, University Park, PA, 16802 USA
E-mail: [email protected], [email protected]
Search for more papers by this authorXinyu Tan
Department of Cell Biology, School of Basic Medical Sciences, Southern Medical University, Guangzhou, Guangdong Province, 510515 China
Department of Biomedical Engineering, Materials Research Institute, The Huck Institutes of the Life Sciences, The Pennsylvania State University, University Park, PA, 16802 USA
Academy of Orthopedics, Provincial Key Laboratory of Bone and Joint Degenerative Diseases, The Third Affiliated Hospital of Southern Medical University, Guangzhou, Guangdong Province, 510280 China
Search for more papers by this authorEthan Gerhard
Department of Biomedical Engineering, Materials Research Institute, The Huck Institutes of the Life Sciences, The Pennsylvania State University, University Park, PA, 16802 USA
Search for more papers by this authorYuqi Wang
Department of Biomedical Engineering, Materials Research Institute, The Huck Institutes of the Life Sciences, The Pennsylvania State University, University Park, PA, 16802 USA
Search for more papers by this authorRichard T. Tran
Department of Biomedical Engineering, Materials Research Institute, The Huck Institutes of the Life Sciences, The Pennsylvania State University, University Park, PA, 16802 USA
Search for more papers by this authorHui Xu
Department of Biomedical Engineering, Materials Research Institute, The Huck Institutes of the Life Sciences, The Pennsylvania State University, University Park, PA, 16802 USA
Search for more papers by this authorSu Yan
Department of Biomedical Engineering, Materials Research Institute, The Huck Institutes of the Life Sciences, The Pennsylvania State University, University Park, PA, 16802 USA
Search for more papers by this authorElias B. Rizk
Department of Neurosurgery, College of Medicine, The Pennsylvania State University, Hershey, PA, 17033 USA
Search for more papers by this authorApril D. Armstrong
Department of Orthopaedics and Rehabilitation, College of Medicine, The Pennsylvania State University, Hershey, PA, 17033 USA
Search for more papers by this authorYuxiao Zhou
Department of Mechanical Engineering, The Pennsylvania State University, University Park, PA, 16802 USA
Search for more papers by this authorJing Du
Department of Mechanical Engineering, The Pennsylvania State University, University Park, PA, 16802 USA
Search for more papers by this authorCorresponding Author
Xiaochun Bai
Department of Cell Biology, School of Basic Medical Sciences, Southern Medical University, Guangzhou, Guangdong Province, 510515 China
Academy of Orthopedics, Provincial Key Laboratory of Bone and Joint Degenerative Diseases, The Third Affiliated Hospital of Southern Medical University, Guangzhou, Guangdong Province, 510280 China
E-mail: [email protected], [email protected]
Search for more papers by this authorCorresponding Author
Jian Yang
Department of Biomedical Engineering, Materials Research Institute, The Huck Institutes of the Life Sciences, The Pennsylvania State University, University Park, PA, 16802 USA
E-mail: [email protected], [email protected]
Search for more papers by this authorAbstract
The burden of bone fractures demands development of effective biomaterial solutions, while additional acute events such as noncompressible bleeding further motivate the search for multi-functional implants to avoid complications including osseous hemorrhage, infection, and nonunion. Bone wax has been widely used in orthopedic bleeding control due to its simplicity of use and conformation to irregular defects; however, its nondegradability results in impaired bone healing, risk of infection, and significant inflammatory responses. Herein, a class of intrinsically fluorescent, osteopromotive citrate-based polymer/hydroxyapatite (HA) composites (BPLP-Ser/HA) as a highly malleable press-fit putty is designed. BPLP-Ser/HA putty displays mechanics replicating early nonmineralized bone (initial moduli from ≈2–500 kPa), hydration induced mechanical strengthening in physiological conditions, tunable degradation rates (over 2 months), low swelling ratios (<10%), clotting and hemostatic sealing potential (resistant to blood pressure for >24 h) and significant adhesion to bone (≈350–550 kPa). Simultaneously, citrate's bioactive properties result in antimicrobial (≈100% and 55% inhibition of S. aureus and E. coli) and osteopromotive effects. Finally, BPLP-Ser/HA putty demonstrates in vivo regeneration in a critical-sized rat calvaria model equivalent to gold standard autograft. BPLP-Ser/HA putty represents a simple, off-the-shelf solution to the combined challenges of acute wound management and subsequent bone regeneration.
Conflict of Interest
Dr. Jian Yang and The Pennsylvania State University have a financial interest in Acuitive Technologies, Inc. and Aleo BME, Inc. These interests have been reviewed by the University's Institutional and Individual Conflict of Interest Committees and are currently being managed by the University.
Open Research
Data Availability Statement
Research data are not shared.
Supporting Information
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