Laser-Assisted Fabrication of Injectable Nanofibrous Cell Carriers
Corresponding Author
Paweł Nakielski
Department of Biosystems and Soft Matter, Institute of Fundamental Technological Research, Polish Academy of Sciences, Warsaw, 02-106 Poland
E-mail: [email protected], [email protected]
Search for more papers by this authorChiara Rinoldi
Department of Biosystems and Soft Matter, Institute of Fundamental Technological Research, Polish Academy of Sciences, Warsaw, 02-106 Poland
Search for more papers by this authorMichał Pruchniewski
Department of Biosystems and Soft Matter, Institute of Fundamental Technological Research, Polish Academy of Sciences, Warsaw, 02-106 Poland
Department of Nanobiotechnology, Institute of Biology, Warsaw University of Life Sciences, Warsaw, 02-787 Poland
Search for more papers by this authorSylwia Pawłowska
Department of Biosystems and Soft Matter, Institute of Fundamental Technological Research, Polish Academy of Sciences, Warsaw, 02-106 Poland
Search for more papers by this authorMałgorzata Gazińska
Department of Engineering and Technology of Polymers, Wrocław University of Science and Technology, Wrocław, 50-370 Poland
Search for more papers by this authorBarbara Strojny
Department of Nanobiotechnology, Institute of Biology, Warsaw University of Life Sciences, Warsaw, 02-787 Poland
Search for more papers by this authorDaniel Rybak
Department of Biosystems and Soft Matter, Institute of Fundamental Technological Research, Polish Academy of Sciences, Warsaw, 02-106 Poland
Search for more papers by this authorKatarzyna Jezierska-Woźniak
Laboratory for Regenerative Medicine, Department of Neurosurgery, School of Medicine, University of Warmia and Mazury in Olsztyn, Olsztyn, 10-082 Poland
Search for more papers by this authorOlga Urbanek
Laboratory of Polymers and Biomaterials, Institute of Fundamental Technological Research, Polish Academy of Sciences, Warsaw, 02-106 Poland
Search for more papers by this authorPiotr Denis
Laboratory of Polymers and Biomaterials, Institute of Fundamental Technological Research, Polish Academy of Sciences, Warsaw, 02-106 Poland
Search for more papers by this authorEmilia Sinderewicz
Laboratory for Regenerative Medicine, Department of Neurosurgery, School of Medicine, University of Warmia and Mazury in Olsztyn, Olsztyn, 10-082 Poland
Search for more papers by this authorWioleta Czelejewska
Laboratory for Regenerative Medicine, Department of Neurosurgery, School of Medicine, University of Warmia and Mazury in Olsztyn, Olsztyn, 10-082 Poland
Search for more papers by this authorJoanna Staszkiewicz-Chodor
Laboratory for Regenerative Medicine, Department of Neurosurgery, School of Medicine, University of Warmia and Mazury in Olsztyn, Olsztyn, 10-082 Poland
Search for more papers by this authorMarta Grodzik
Department of Nanobiotechnology, Institute of Biology, Warsaw University of Life Sciences, Warsaw, 02-787 Poland
Search for more papers by this authorYasamin Ziai
Department of Biosystems and Soft Matter, Institute of Fundamental Technological Research, Polish Academy of Sciences, Warsaw, 02-106 Poland
Search for more papers by this authorMonika Barczewska
Laboratory for Regenerative Medicine, Department of Neurosurgery, School of Medicine, University of Warmia and Mazury in Olsztyn, Olsztyn, 10-082 Poland
Search for more papers by this authorWojciech Maksymowicz
Laboratory for Regenerative Medicine, Department of Neurosurgery, School of Medicine, University of Warmia and Mazury in Olsztyn, Olsztyn, 10-082 Poland
Search for more papers by this authorCorresponding Author
Filippo Pierini
Department of Biosystems and Soft Matter, Institute of Fundamental Technological Research, Polish Academy of Sciences, Warsaw, 02-106 Poland
E-mail: [email protected], [email protected]
Search for more papers by this authorCorresponding Author
Paweł Nakielski
Department of Biosystems and Soft Matter, Institute of Fundamental Technological Research, Polish Academy of Sciences, Warsaw, 02-106 Poland
E-mail: [email protected], [email protected]
Search for more papers by this authorChiara Rinoldi
Department of Biosystems and Soft Matter, Institute of Fundamental Technological Research, Polish Academy of Sciences, Warsaw, 02-106 Poland
Search for more papers by this authorMichał Pruchniewski
Department of Biosystems and Soft Matter, Institute of Fundamental Technological Research, Polish Academy of Sciences, Warsaw, 02-106 Poland
Department of Nanobiotechnology, Institute of Biology, Warsaw University of Life Sciences, Warsaw, 02-787 Poland
Search for more papers by this authorSylwia Pawłowska
Department of Biosystems and Soft Matter, Institute of Fundamental Technological Research, Polish Academy of Sciences, Warsaw, 02-106 Poland
Search for more papers by this authorMałgorzata Gazińska
Department of Engineering and Technology of Polymers, Wrocław University of Science and Technology, Wrocław, 50-370 Poland
Search for more papers by this authorBarbara Strojny
Department of Nanobiotechnology, Institute of Biology, Warsaw University of Life Sciences, Warsaw, 02-787 Poland
Search for more papers by this authorDaniel Rybak
Department of Biosystems and Soft Matter, Institute of Fundamental Technological Research, Polish Academy of Sciences, Warsaw, 02-106 Poland
Search for more papers by this authorKatarzyna Jezierska-Woźniak
Laboratory for Regenerative Medicine, Department of Neurosurgery, School of Medicine, University of Warmia and Mazury in Olsztyn, Olsztyn, 10-082 Poland
Search for more papers by this authorOlga Urbanek
Laboratory of Polymers and Biomaterials, Institute of Fundamental Technological Research, Polish Academy of Sciences, Warsaw, 02-106 Poland
Search for more papers by this authorPiotr Denis
Laboratory of Polymers and Biomaterials, Institute of Fundamental Technological Research, Polish Academy of Sciences, Warsaw, 02-106 Poland
Search for more papers by this authorEmilia Sinderewicz
Laboratory for Regenerative Medicine, Department of Neurosurgery, School of Medicine, University of Warmia and Mazury in Olsztyn, Olsztyn, 10-082 Poland
Search for more papers by this authorWioleta Czelejewska
Laboratory for Regenerative Medicine, Department of Neurosurgery, School of Medicine, University of Warmia and Mazury in Olsztyn, Olsztyn, 10-082 Poland
Search for more papers by this authorJoanna Staszkiewicz-Chodor
Laboratory for Regenerative Medicine, Department of Neurosurgery, School of Medicine, University of Warmia and Mazury in Olsztyn, Olsztyn, 10-082 Poland
Search for more papers by this authorMarta Grodzik
Department of Nanobiotechnology, Institute of Biology, Warsaw University of Life Sciences, Warsaw, 02-787 Poland
Search for more papers by this authorYasamin Ziai
Department of Biosystems and Soft Matter, Institute of Fundamental Technological Research, Polish Academy of Sciences, Warsaw, 02-106 Poland
Search for more papers by this authorMonika Barczewska
Laboratory for Regenerative Medicine, Department of Neurosurgery, School of Medicine, University of Warmia and Mazury in Olsztyn, Olsztyn, 10-082 Poland
Search for more papers by this authorWojciech Maksymowicz
Laboratory for Regenerative Medicine, Department of Neurosurgery, School of Medicine, University of Warmia and Mazury in Olsztyn, Olsztyn, 10-082 Poland
Search for more papers by this authorCorresponding Author
Filippo Pierini
Department of Biosystems and Soft Matter, Institute of Fundamental Technological Research, Polish Academy of Sciences, Warsaw, 02-106 Poland
E-mail: [email protected], [email protected]
Search for more papers by this authorAbstract
The use of injectable biomaterials for cell delivery is a rapidly expanding field which may revolutionize the medical treatments by making them less invasive. However, creating desirable cell carriers poses significant challenges to the clinical implementation of cell-based therapeutics. At the same time, no method has been developed to produce injectable microscaffolds (MSs) from electrospun materials. Here the fabrication of injectable electrospun nanofibers is reported on, which retain their fibrous structure to mimic the extracellular matrix. The laser-assisted micro-scaffold fabrication has produced tens of thousands of MSs in a short time. An efficient attachment of cells to the surface and their proliferation is observed, creating cell-populated MSs. The cytocompatibility assays proved their biocompatibility, safety, and potential as cell carriers. Ex vivo results with the use of bone and cartilage tissues proved that NaOH hydrolyzed and chitosan functionalized MSs are compatible with living tissues and readily populated with cells. Injectability studies of MSs showed a high injectability rate, while at the same time, the force needed to eject the load is no higher than 25 N. In the future, the produced MSs may be studied more in-depth as cell carriers in minimally invasive cell therapies and 3D bioprinting applications.
Conflict of Interest
The authors declare no conflict of interest.
Open Research
Data Availability Statement
Research data are not shared.
Supporting Information
| Filename | Description |
|---|---|
| smll202104971-sup-0001-SuppMat.pdf1.6 MB | Supporting Information |
| smll202104971-sup-0002-MovieS1.mp430.2 MB | Supplemental Movie 1 |
| smll202104971-sup-0003-MovieS2.mp4501.5 KB | Supplemental Movie 2 |
| smll202104971-sup-0004-MovieS3.mp41.5 MB | Supplemental Movie 3 |
| smll202104971-sup-0005-MovieS4.mp410.9 MB | Supplemental Movie 4 |
Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.
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