Sequential Multitrack Nonlinear Ex vivo Imaging of Esophageal Stroma Based on Backscattered Second-harmonic Generation and Two-photon Autofluorescence
Shuangmu Zhuo
Key Laboratory of Optoelectronic Science and Technology for Medicine, (Fujian Normal University), Ministry of Education, Fuzhou 350007, China
Search for more papers by this authorCorresponding Author
Jianxin Chen
Key Laboratory of Optoelectronic Science and Technology for Medicine, (Fujian Normal University), Ministry of Education, Fuzhou 350007, China
Jianxin Chen, Key Laboratory of Optoelectronic Science and Technology for Medicine, (Fujian Normal University), Ministry of Education, Fuzhou 350007, ChinaSearch for more papers by this authorXingshan Jiang
Key Laboratory of Optoelectronic Science and Technology for Medicine, (Fujian Normal University), Ministry of Education, Fuzhou 350007, China
Search for more papers by this authorTianshu Luo
Key Laboratory of Optoelectronic Science and Technology for Medicine, (Fujian Normal University), Ministry of Education, Fuzhou 350007, China
Search for more papers by this authorRong Chen
Key Laboratory of Optoelectronic Science and Technology for Medicine, (Fujian Normal University), Ministry of Education, Fuzhou 350007, China
Search for more papers by this authorShusen Xie
Key Laboratory of Optoelectronic Science and Technology for Medicine, (Fujian Normal University), Ministry of Education, Fuzhou 350007, China
Search for more papers by this authorQilian Zou
Division of cell biology and genetics, Fujian Medical University, Fuzhou 350004, China
Search for more papers by this authorShuangmu Zhuo
Key Laboratory of Optoelectronic Science and Technology for Medicine, (Fujian Normal University), Ministry of Education, Fuzhou 350007, China
Search for more papers by this authorCorresponding Author
Jianxin Chen
Key Laboratory of Optoelectronic Science and Technology for Medicine, (Fujian Normal University), Ministry of Education, Fuzhou 350007, China
Jianxin Chen, Key Laboratory of Optoelectronic Science and Technology for Medicine, (Fujian Normal University), Ministry of Education, Fuzhou 350007, ChinaSearch for more papers by this authorXingshan Jiang
Key Laboratory of Optoelectronic Science and Technology for Medicine, (Fujian Normal University), Ministry of Education, Fuzhou 350007, China
Search for more papers by this authorTianshu Luo
Key Laboratory of Optoelectronic Science and Technology for Medicine, (Fujian Normal University), Ministry of Education, Fuzhou 350007, China
Search for more papers by this authorRong Chen
Key Laboratory of Optoelectronic Science and Technology for Medicine, (Fujian Normal University), Ministry of Education, Fuzhou 350007, China
Search for more papers by this authorShusen Xie
Key Laboratory of Optoelectronic Science and Technology for Medicine, (Fujian Normal University), Ministry of Education, Fuzhou 350007, China
Search for more papers by this authorQilian Zou
Division of cell biology and genetics, Fujian Medical University, Fuzhou 350004, China
Search for more papers by this authorAbstract
We demonstrate the technique of subsequent multitrack nonlinear imaging based on backscattered second-harmonic generation (B-SHG) and two-photon autofluorescence (TPA) to obtain large-area, high-contrast, submicron-resolution image ex vivo of esophageal stroma. Our findings show that this technique is effective in improving the B-SHG/TPA image contrast. It was found that the method can quantitatively obtain microscopic structural and biochemical information on stroma. Our work suggests that the technique has the potential to provide accurate and comprehensive information in determining the physiological and pathological states of the esophagus. SCANNING 29: 219-224, 2007. © 2007 Wiley Periodicals, Inc.
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