Capilliposide B inhibits the migration of prostate cancer by inducing autophagy through the ROS/AMPK/mTOR pathway
Luping Wang
College of Biomedical Engineering & Instrument Science, Zhejiang University, Hangzhou, People's Republic of China
Institute of Basic Medicine and Cancer, Chinese Academy of Sciences, Cancer Hospital of the University of Chinese Academy of Sciences, Zhejiang Cancer Hospital, Hangzhou, People's Republic of China
Search for more papers by this authorHaote Han
College of Biomedical Engineering & Instrument Science, Zhejiang University, Hangzhou, People's Republic of China
Search for more papers by this authorYue Feng
Institute of Basic Medicine and Cancer, Chinese Academy of Sciences, Cancer Hospital of the University of Chinese Academy of Sciences, Zhejiang Cancer Hospital, Hangzhou, People's Republic of China
Search for more papers by this authorJiahui Ma
Marine Science College, Zhejiang Ocean University, Zhoushan, Zhejiang, People's Republic of China
Search for more papers by this authorZhuo Han
Institute of Basic Medicine and Cancer, Chinese Academy of Sciences, Cancer Hospital of the University of Chinese Academy of Sciences, Zhejiang Cancer Hospital, Hangzhou, People's Republic of China
Search for more papers by this authorRuyi Li
College of Biomedical Engineering & Instrument Science, Zhejiang University, Hangzhou, People's Republic of China
Search for more papers by this authorWei Zhu
Institute of Basic Medicine and Cancer, Chinese Academy of Sciences, Cancer Hospital of the University of Chinese Academy of Sciences, Zhejiang Cancer Hospital, Hangzhou, People's Republic of China
Search for more papers by this authorShouxin Li
Institute of Basic Medicine and Cancer, Chinese Academy of Sciences, Cancer Hospital of the University of Chinese Academy of Sciences, Zhejiang Cancer Hospital, Hangzhou, People's Republic of China
Search for more papers by this authorCorresponding Author
Jingkui Tian
Institute of Basic Medicine and Cancer, Chinese Academy of Sciences, Cancer Hospital of the University of Chinese Academy of Sciences, Zhejiang Cancer Hospital, Hangzhou, People's Republic of China
Correspondence
Lin Zhang, College of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou 310018, People's Republic of China.
Email: [email protected]
Jingkui Tian, Institute of Basic Medicine and Cancer, Chinese Academy of Sciences, Cancer Hospital of the University of Chinese Academy of Sciences, Zhejiang Cancer Hospital, Hangzhou, People's Republic of China.
Email: [email protected]
Search for more papers by this authorCorresponding Author
Lin Zhang
College of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou, People's Republic of China
Correspondence
Lin Zhang, College of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou 310018, People's Republic of China.
Email: [email protected]
Jingkui Tian, Institute of Basic Medicine and Cancer, Chinese Academy of Sciences, Cancer Hospital of the University of Chinese Academy of Sciences, Zhejiang Cancer Hospital, Hangzhou, People's Republic of China.
Email: [email protected]
Search for more papers by this authorLuping Wang
College of Biomedical Engineering & Instrument Science, Zhejiang University, Hangzhou, People's Republic of China
Institute of Basic Medicine and Cancer, Chinese Academy of Sciences, Cancer Hospital of the University of Chinese Academy of Sciences, Zhejiang Cancer Hospital, Hangzhou, People's Republic of China
Search for more papers by this authorHaote Han
College of Biomedical Engineering & Instrument Science, Zhejiang University, Hangzhou, People's Republic of China
Search for more papers by this authorYue Feng
Institute of Basic Medicine and Cancer, Chinese Academy of Sciences, Cancer Hospital of the University of Chinese Academy of Sciences, Zhejiang Cancer Hospital, Hangzhou, People's Republic of China
Search for more papers by this authorJiahui Ma
Marine Science College, Zhejiang Ocean University, Zhoushan, Zhejiang, People's Republic of China
Search for more papers by this authorZhuo Han
Institute of Basic Medicine and Cancer, Chinese Academy of Sciences, Cancer Hospital of the University of Chinese Academy of Sciences, Zhejiang Cancer Hospital, Hangzhou, People's Republic of China
Search for more papers by this authorRuyi Li
College of Biomedical Engineering & Instrument Science, Zhejiang University, Hangzhou, People's Republic of China
Search for more papers by this authorWei Zhu
Institute of Basic Medicine and Cancer, Chinese Academy of Sciences, Cancer Hospital of the University of Chinese Academy of Sciences, Zhejiang Cancer Hospital, Hangzhou, People's Republic of China
Search for more papers by this authorShouxin Li
Institute of Basic Medicine and Cancer, Chinese Academy of Sciences, Cancer Hospital of the University of Chinese Academy of Sciences, Zhejiang Cancer Hospital, Hangzhou, People's Republic of China
Search for more papers by this authorCorresponding Author
Jingkui Tian
Institute of Basic Medicine and Cancer, Chinese Academy of Sciences, Cancer Hospital of the University of Chinese Academy of Sciences, Zhejiang Cancer Hospital, Hangzhou, People's Republic of China
Correspondence
Lin Zhang, College of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou 310018, People's Republic of China.
Email: [email protected]
Jingkui Tian, Institute of Basic Medicine and Cancer, Chinese Academy of Sciences, Cancer Hospital of the University of Chinese Academy of Sciences, Zhejiang Cancer Hospital, Hangzhou, People's Republic of China.
Email: [email protected]
Search for more papers by this authorCorresponding Author
Lin Zhang
College of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou, People's Republic of China
Correspondence
Lin Zhang, College of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou 310018, People's Republic of China.
Email: [email protected]
Jingkui Tian, Institute of Basic Medicine and Cancer, Chinese Academy of Sciences, Cancer Hospital of the University of Chinese Academy of Sciences, Zhejiang Cancer Hospital, Hangzhou, People's Republic of China.
Email: [email protected]
Search for more papers by this authorLuping Wang, Haote Han, and Yue Feng all equally contributed to this study.
Abstract
Capilliposide B (CPS-B), a novel oleanane triterpenoid saponin derived from Lysimachia capillipes Hemsl, is a potent anticancer agent. However, its anticancer mechanism remains elusive. In the present study, we demonstrated the potent anti-tumor activity and molecular mechanism of CPS-B both in vitro and in vivo. Proteomic analysis using isobaric tags for relative and absolute quantitation techniques suggested that CPS-B modulated autophagy in prostate cancer (PC). Moreover, Western blotting showed that both autophagy and epithelial–mesenchymal transition occurred place after CPS-B treatment in vivo, which was also proven in PC-3 cancer cells. We deduced that CPS-B inhibited migration by inducing autophagy. We examined the accumulation of reactive oxygen species (ROS) in cells, and in downstream pathways, LKB1 and AMPK were activated while mTOR was inhibited. Transwell experiment results showed that CPS-B inhibited the metastasis of PC-3 cells and that this effect was significantly attenuated after pretreatment with chloroquine, indicating that CPS-B inhibited metastasis via autophagy induction. Altogether, these data suggest that CPS-B is a potential therapeutic agent for cancer treatment that acts by inhibiting migration through the ROS/AMPK/mTOR signaling pathway.
CONFLICT OF INTEREST STATEMENT
The authors declare no conflicts of interest.
Open Research
DATA AVAILABILITY STATEMENT
The data that supports the findings of this study are available in the supplementary material of this article.
Supporting Information
| Filename | Description |
|---|---|
| ptr7785-sup-0001-TableS1.docxWord 2007 document , 1.2 MB | Data S1. Supporting information. |
Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.
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