Comparative Study of Different Acorus Species in Potentiating Neuronal Differentiation in Cultured PC12 Cells
Kelly Y.C. Lam
Division of Life Science, Center for Chinese Medicine, The Hong Kong University of Science and Technology, Clear Water Bay, Hong Kong
Search for more papers by this authorYun Huang
Division of Life Science, Center for Chinese Medicine, The Hong Kong University of Science and Technology, Clear Water Bay, Hong Kong
Search for more papers by this authorPing Yao
Division of Life Science, Center for Chinese Medicine, The Hong Kong University of Science and Technology, Clear Water Bay, Hong Kong
Search for more papers by this authorHuaiyou Wang
Division of Life Science, Center for Chinese Medicine, The Hong Kong University of Science and Technology, Clear Water Bay, Hong Kong
HKUST Shenzhen Research Institute, Hi-Tech Park, Nanshan, Shenzhen, Guangdong Province, China
Search for more papers by this authorTina T.X. Dong
Division of Life Science, Center for Chinese Medicine, The Hong Kong University of Science and Technology, Clear Water Bay, Hong Kong
HKUST Shenzhen Research Institute, Hi-Tech Park, Nanshan, Shenzhen, Guangdong Province, China
Search for more papers by this authorZhongyu Zhou
Division of Life Science, Center for Chinese Medicine, The Hong Kong University of Science and Technology, Clear Water Bay, Hong Kong
Key Laboratory of Plant Resources Conservation and Sustainable Utilization, Guangdong Provincial Key Laboratory of Applied Botany, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou, China
Search for more papers by this authorCorresponding Author
Karl W.K. Tsim
Division of Life Science, Center for Chinese Medicine, The Hong Kong University of Science and Technology, Clear Water Bay, Hong Kong
HKUST Shenzhen Research Institute, Hi-Tech Park, Nanshan, Shenzhen, Guangdong Province, China
Correspondence to: Professor Karl W.K. Tsim, Division of Life Science, Center for Chinese Medicine The Hong Kong University of Science and Technology, Clear Water Bay Road, Hong Kong SAR, China.
E-mail: [email protected]
Search for more papers by this authorKelly Y.C. Lam
Division of Life Science, Center for Chinese Medicine, The Hong Kong University of Science and Technology, Clear Water Bay, Hong Kong
Search for more papers by this authorYun Huang
Division of Life Science, Center for Chinese Medicine, The Hong Kong University of Science and Technology, Clear Water Bay, Hong Kong
Search for more papers by this authorPing Yao
Division of Life Science, Center for Chinese Medicine, The Hong Kong University of Science and Technology, Clear Water Bay, Hong Kong
Search for more papers by this authorHuaiyou Wang
Division of Life Science, Center for Chinese Medicine, The Hong Kong University of Science and Technology, Clear Water Bay, Hong Kong
HKUST Shenzhen Research Institute, Hi-Tech Park, Nanshan, Shenzhen, Guangdong Province, China
Search for more papers by this authorTina T.X. Dong
Division of Life Science, Center for Chinese Medicine, The Hong Kong University of Science and Technology, Clear Water Bay, Hong Kong
HKUST Shenzhen Research Institute, Hi-Tech Park, Nanshan, Shenzhen, Guangdong Province, China
Search for more papers by this authorZhongyu Zhou
Division of Life Science, Center for Chinese Medicine, The Hong Kong University of Science and Technology, Clear Water Bay, Hong Kong
Key Laboratory of Plant Resources Conservation and Sustainable Utilization, Guangdong Provincial Key Laboratory of Applied Botany, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou, China
Search for more papers by this authorCorresponding Author
Karl W.K. Tsim
Division of Life Science, Center for Chinese Medicine, The Hong Kong University of Science and Technology, Clear Water Bay, Hong Kong
HKUST Shenzhen Research Institute, Hi-Tech Park, Nanshan, Shenzhen, Guangdong Province, China
Correspondence to: Professor Karl W.K. Tsim, Division of Life Science, Center for Chinese Medicine The Hong Kong University of Science and Technology, Clear Water Bay Road, Hong Kong SAR, China.
E-mail: [email protected]
Search for more papers by this authorAbstract
Acori Tatarinowii Rhizoma (ATR), the rhizome of Acorus tatarinowii Schott, is a common traditional Chinese medicine being used clinically for mental disorder. However, other Acorus species herbs are all having the same Chinese name ‘Chang Pu’, making the confusion in herbal market. Acori Graminei Rhizoma (AGR) and Acori Calami Rhizoma (ACR) are common adulterants of ATR. Here, we aim to provide a comparative analysis between ATR, AGR, and ACR in potentiating neuronal differentiation. Volatile oil, derived from Acorus species, was applied onto cultured PC12 cells, and various parameters were determined: (i) transcriptional activation of neurofilament promoters was determined by the promoter-driven luciferase activity assay; (ii) the neurite outgrowth of PC12 cells was captured and measured; and (iii) the neurofilament expression and its underlying mechanism were analyzed by western blotting. The co-treatment of ATR, AGR, or ACR volatile oil with low concentration of nerve growth factor (NGF) could potentiate the NGF-induced neuronal differentiation in cultured PC12 cells. In addition, application of protein kinase A inhibitor H89 in cultures blocked the induction of neurofilament. Among these three Acorus species, ATR volatile oil showed the highest NGF-induced induction in neurite outgrowth and neurofilament expression, as compared with that of AGR and ACR. Copyright © 2017 John Wiley & Sons, Ltd.
Supporting Information
| Filename | Description |
|---|---|
| ptr5904-supp-0001Fig_S1.tifTIFF image, 8.4 MB |
Fig. S1 Effect of Acori Tatarinowii Rhizoma, Acori Graminei Rhizoma, or Acori Calami Rhizoma volatile oil on the viability of PC12 cells. Cultured PC12 cells were treated with the different doses (0.3 to 300 μg/mL) of Acori Tatarinowii Rhizoma, Acori Graminei Rhizoma, or Acori Calami Rhizoma volatile oil for 48 h. Cell viability (using the colorimetric MTT assay) was performed. No significant increase in cell viability was observed. Values were expressed in percentage of control in cell number and in mean ± SEM, n = 5, each with triplicate samples. |
Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.
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