Stilbenoids from Rheum undulatum Protect Hepatocytes Against Oxidative Stress Through AMPK Activation
Guang-zhi Dong
Research Center for Cell Fate Control and College of Pharmacy, Sookmyung Women's University, 52 Hyochangwon-Gil, Yongsan-Gu, Seoul, 140-742 Republic of Korea
Search for more papers by this authorYu-ih Lee
Research Center for Cell Fate Control and College of Pharmacy, Sookmyung Women's University, 52 Hyochangwon-Gil, Yongsan-Gu, Seoul, 140-742 Republic of Korea
Search for more papers by this authorJi Hye Jeong
Research Center for Cell Fate Control and College of Pharmacy, Sookmyung Women's University, 52 Hyochangwon-Gil, Yongsan-Gu, Seoul, 140-742 Republic of Korea
Search for more papers by this authorHui-Yuan Zhao
Research Center for Cell Fate Control and College of Pharmacy, Sookmyung Women's University, 52 Hyochangwon-Gil, Yongsan-Gu, Seoul, 140-742 Republic of Korea
Search for more papers by this authorRaok Jeon
Research Center for Cell Fate Control and College of Pharmacy, Sookmyung Women's University, 52 Hyochangwon-Gil, Yongsan-Gu, Seoul, 140-742 Republic of Korea
Search for more papers by this authorHwa Jin Lee
Department of Natural Medicine Resources, Semyung University, 65 Semyung-ro, Jecheon, Chungbuk, 390-711 Republic of Korea
Search for more papers by this authorCorresponding Author
Jae-Ha Ryu
Research Center for Cell Fate Control and College of Pharmacy, Sookmyung Women's University, 52 Hyochangwon-Gil, Yongsan-Gu, Seoul, 140-742 Republic of Korea
Correspondence to: Jae-Ha Ryu, Research Center for Cell Fate Control and College of Pharmacy, Sookmyung Women's University, 52 Hyochangwon-Gil, Yongsan-Gu, Seoul 140-742, Republic of Korea.
E-mail: [email protected]
Search for more papers by this authorGuang-zhi Dong
Research Center for Cell Fate Control and College of Pharmacy, Sookmyung Women's University, 52 Hyochangwon-Gil, Yongsan-Gu, Seoul, 140-742 Republic of Korea
Search for more papers by this authorYu-ih Lee
Research Center for Cell Fate Control and College of Pharmacy, Sookmyung Women's University, 52 Hyochangwon-Gil, Yongsan-Gu, Seoul, 140-742 Republic of Korea
Search for more papers by this authorJi Hye Jeong
Research Center for Cell Fate Control and College of Pharmacy, Sookmyung Women's University, 52 Hyochangwon-Gil, Yongsan-Gu, Seoul, 140-742 Republic of Korea
Search for more papers by this authorHui-Yuan Zhao
Research Center for Cell Fate Control and College of Pharmacy, Sookmyung Women's University, 52 Hyochangwon-Gil, Yongsan-Gu, Seoul, 140-742 Republic of Korea
Search for more papers by this authorRaok Jeon
Research Center for Cell Fate Control and College of Pharmacy, Sookmyung Women's University, 52 Hyochangwon-Gil, Yongsan-Gu, Seoul, 140-742 Republic of Korea
Search for more papers by this authorHwa Jin Lee
Department of Natural Medicine Resources, Semyung University, 65 Semyung-ro, Jecheon, Chungbuk, 390-711 Republic of Korea
Search for more papers by this authorCorresponding Author
Jae-Ha Ryu
Research Center for Cell Fate Control and College of Pharmacy, Sookmyung Women's University, 52 Hyochangwon-Gil, Yongsan-Gu, Seoul, 140-742 Republic of Korea
Correspondence to: Jae-Ha Ryu, Research Center for Cell Fate Control and College of Pharmacy, Sookmyung Women's University, 52 Hyochangwon-Gil, Yongsan-Gu, Seoul 140-742, Republic of Korea.
E-mail: [email protected]
Search for more papers by this authorAbstract
Oxidative stress promotes several diseases, including liver disease. We have isolated several stilbenoids from Rheum undulatum to investigate their hepatoprotective activities and mechanism. Stilbenoids from R. undulatum protects hepatocytes against arachidonic acid + iron (AA + Fe) induced oxidative stress. Pterostilbene (compound 5) shows stronger activity than the others. Trimethoxystilbenoid (compound 6) shows best activity on protection of HepG2 cells from AA + Fe-induced oxidative stress, and trans-stilbenoid (compound 7) shows weak activity. These stilbenoids suppress ROS generation in AA + Fe-treated HepG2 cells and also suppress AA + Fe-induced MMP disruption. Their protective effects on AA + Fe-induced MMP disruption were abrogated by treatment of AMP-activated protein kinase (AMPK) inhibitor, compound C or transfection of dominant negative form of AMPK. Taken together, stilbenoids from R. undulatum protect hepatocytes against AA + Fe-induced oxidative stress through AMPK activation. And the methoxy groups in the aryl groups are important for their cytoprotective activity. Copyright © 2015 John Wiley & Sons, Ltd.
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