Lupeol Protects Against Cerulein-Induced Acute Pancreatitis in Mice
Min-Jun Kim
Department of Herbology, School of Oriental Medicine, Wonkwang University, Iksan, Jeonbuk, 540-749 Republic of Korea
BK21 Plus Team, Professional Graduate School of Oriental medicine, Wonkwang University, Iksan, Jeonbuk, 540-749 Republic of Korea
These authors contributed equally to this work.Search for more papers by this authorGi-Sang Bae
Department of Herbology, School of Oriental Medicine, Wonkwang University, Iksan, Jeonbuk, 540-749 Republic of Korea
Hanbang Body-Fluid Research Center, Wonkwang University, Iksan, Jeonbuk, 540-749 Republic of Korea
These authors contributed equally to this work.Search for more papers by this authorSun Bok Choi
Department of Herbology, School of Oriental Medicine, Wonkwang University, Iksan, Jeonbuk, 540-749 Republic of Korea
BK21 Plus Team, Professional Graduate School of Oriental medicine, Wonkwang University, Iksan, Jeonbuk, 540-749 Republic of Korea
Search for more papers by this authorIl-Joo Jo
Department of Herbology, School of Oriental Medicine, Wonkwang University, Iksan, Jeonbuk, 540-749 Republic of Korea
BK21 Plus Team, Professional Graduate School of Oriental medicine, Wonkwang University, Iksan, Jeonbuk, 540-749 Republic of Korea
Search for more papers by this authorDong-Goo Kim
Department of Herbology, School of Oriental Medicine, Wonkwang University, Iksan, Jeonbuk, 540-749 Republic of Korea
BK21 Plus Team, Professional Graduate School of Oriental medicine, Wonkwang University, Iksan, Jeonbuk, 540-749 Republic of Korea
Search for more papers by this authorJoon-Yeon Shin
Department of Herbology, School of Oriental Medicine, Wonkwang University, Iksan, Jeonbuk, 540-749 Republic of Korea
BK21 Plus Team, Professional Graduate School of Oriental medicine, Wonkwang University, Iksan, Jeonbuk, 540-749 Republic of Korea
Search for more papers by this authorSung-Kon Lee
Department of Herbology, School of Oriental Medicine, Wonkwang University, Iksan, Jeonbuk, 540-749 Republic of Korea
BK21 Plus Team, Professional Graduate School of Oriental medicine, Wonkwang University, Iksan, Jeonbuk, 540-749 Republic of Korea
Search for more papers by this authorMyoung-Jin Kim
Department of Herbology, School of Oriental Medicine, Wonkwang University, Iksan, Jeonbuk, 540-749 Republic of Korea
BK21 Plus Team, Professional Graduate School of Oriental medicine, Wonkwang University, Iksan, Jeonbuk, 540-749 Republic of Korea
Search for more papers by this authorHo-Joon Song
Department of Herbology, School of Oriental Medicine, Wonkwang University, Iksan, Jeonbuk, 540-749 Republic of Korea
BK21 Plus Team, Professional Graduate School of Oriental medicine, Wonkwang University, Iksan, Jeonbuk, 540-749 Republic of Korea
Search for more papers by this authorCorresponding Author
Sung-Joo Park
Department of Herbology, School of Oriental Medicine, Wonkwang University, Iksan, Jeonbuk, 540-749 Republic of Korea
BK21 Plus Team, Professional Graduate School of Oriental medicine, Wonkwang University, Iksan, Jeonbuk, 540-749 Republic of Korea
Hanbang Body-Fluid Research Center, Wonkwang University, Iksan, Jeonbuk, 540-749 Republic of Korea
Correspondence to: Sung-Joo Park, Department of Herbology, School of Oriental Medicine, Wonkwang University, Iksan, Jeonbuk 540-749, Republic of Korea.
E-mail: [email protected]
Search for more papers by this authorMin-Jun Kim
Department of Herbology, School of Oriental Medicine, Wonkwang University, Iksan, Jeonbuk, 540-749 Republic of Korea
BK21 Plus Team, Professional Graduate School of Oriental medicine, Wonkwang University, Iksan, Jeonbuk, 540-749 Republic of Korea
These authors contributed equally to this work.Search for more papers by this authorGi-Sang Bae
Department of Herbology, School of Oriental Medicine, Wonkwang University, Iksan, Jeonbuk, 540-749 Republic of Korea
Hanbang Body-Fluid Research Center, Wonkwang University, Iksan, Jeonbuk, 540-749 Republic of Korea
These authors contributed equally to this work.Search for more papers by this authorSun Bok Choi
Department of Herbology, School of Oriental Medicine, Wonkwang University, Iksan, Jeonbuk, 540-749 Republic of Korea
BK21 Plus Team, Professional Graduate School of Oriental medicine, Wonkwang University, Iksan, Jeonbuk, 540-749 Republic of Korea
Search for more papers by this authorIl-Joo Jo
Department of Herbology, School of Oriental Medicine, Wonkwang University, Iksan, Jeonbuk, 540-749 Republic of Korea
BK21 Plus Team, Professional Graduate School of Oriental medicine, Wonkwang University, Iksan, Jeonbuk, 540-749 Republic of Korea
Search for more papers by this authorDong-Goo Kim
Department of Herbology, School of Oriental Medicine, Wonkwang University, Iksan, Jeonbuk, 540-749 Republic of Korea
BK21 Plus Team, Professional Graduate School of Oriental medicine, Wonkwang University, Iksan, Jeonbuk, 540-749 Republic of Korea
Search for more papers by this authorJoon-Yeon Shin
Department of Herbology, School of Oriental Medicine, Wonkwang University, Iksan, Jeonbuk, 540-749 Republic of Korea
BK21 Plus Team, Professional Graduate School of Oriental medicine, Wonkwang University, Iksan, Jeonbuk, 540-749 Republic of Korea
Search for more papers by this authorSung-Kon Lee
Department of Herbology, School of Oriental Medicine, Wonkwang University, Iksan, Jeonbuk, 540-749 Republic of Korea
BK21 Plus Team, Professional Graduate School of Oriental medicine, Wonkwang University, Iksan, Jeonbuk, 540-749 Republic of Korea
Search for more papers by this authorMyoung-Jin Kim
Department of Herbology, School of Oriental Medicine, Wonkwang University, Iksan, Jeonbuk, 540-749 Republic of Korea
BK21 Plus Team, Professional Graduate School of Oriental medicine, Wonkwang University, Iksan, Jeonbuk, 540-749 Republic of Korea
Search for more papers by this authorHo-Joon Song
Department of Herbology, School of Oriental Medicine, Wonkwang University, Iksan, Jeonbuk, 540-749 Republic of Korea
BK21 Plus Team, Professional Graduate School of Oriental medicine, Wonkwang University, Iksan, Jeonbuk, 540-749 Republic of Korea
Search for more papers by this authorCorresponding Author
Sung-Joo Park
Department of Herbology, School of Oriental Medicine, Wonkwang University, Iksan, Jeonbuk, 540-749 Republic of Korea
BK21 Plus Team, Professional Graduate School of Oriental medicine, Wonkwang University, Iksan, Jeonbuk, 540-749 Republic of Korea
Hanbang Body-Fluid Research Center, Wonkwang University, Iksan, Jeonbuk, 540-749 Republic of Korea
Correspondence to: Sung-Joo Park, Department of Herbology, School of Oriental Medicine, Wonkwang University, Iksan, Jeonbuk 540-749, Republic of Korea.
E-mail: [email protected]
Search for more papers by this authorAbstract
Lupeol is a triterpenoid commonly found in fruits and vegetables and is known to exhibit a wide range of biological activities, including antiinflammatory and anti-cancer effects. However, the effects of lupeol on acute pancreatitis specifically have not been well characterized. Here, we investigated the effects of lupeol on cerulein-induced acute pancreatitis in mice. Acute pancreatitis was induced via an intraperitoneal injection of cerulein (50 µg/kg). In the lupeol treatment group, lupeol was administered intraperitoneally (10, 25, or 50 mg/kg) 1 h before the first cerulein injection. Blood samples were taken to determine serum cytokine and amylase levels. The pancreas was rapidly removed for morphological examination and used in the myeloperoxidase assay, trypsin activity assay, and real-time reverse transcription polymerase chain reaction. In addition, we isolated pancreatic acinar cells using a collagenase method to examine the acinar cell viability. Lupeol administration significantly attenuated the severity of pancreatitis, as was shown by reduced pancreatic edema, and neutrophil infiltration. In addition, lupeol inhibited elevation of digestive enzymes and cytokine levels, such as tumor necrosis factor (TNF)-α, interleukin (IL)-1, and interleukin (IL)-6. Furthermore, lupeol inhibited the cerulein-induced acinar cell death. In conclusion, these results suggest that lupeol exhibits protective effects on cerulein-induced acute pancreatitis. Copyright © 2015 John Wiley & Sons, Ltd.
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