Volume 24, Issue 12 pp. 1839-1843

Research Article

Radical scavenging and angiotensin converting enzyme inhibitory activities of standardized extracts of Ficus racemosa stem bark

Faiyaz Ahmed,

Corresponding Author

Faiyaz Ahmed

[email protected]

Department of Studies in Food Science and Nutrition, University of Mysore, Mysore-570006, India

Faiyaz Ahmed, Department of Studies in Food Science and Nutrition, University of Mysore, Mysore-570006, India

Bannikuppe S. Vishwanath, Department of Studies in Biochemistry, University of Mysore, Mysore-570006, India

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Jalahalli M. Siddesha,

Jalahalli M. Siddesha

Department of Studies in Biochemistry, University of Mysore, Mysore-570006, India

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Asna Urooj,

Asna Urooj

Department of Studies in Food Science and Nutrition, University of Mysore, Mysore-570006, India

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Bannikuppe S. Vishwanath,

Corresponding Author

Bannikuppe S. Vishwanath

[email protected]

Department of Studies in Biochemistry, University of Mysore, Mysore-570006, India

Faiyaz Ahmed, Department of Studies in Food Science and Nutrition, University of Mysore, Mysore-570006, India

Bannikuppe S. Vishwanath, Department of Studies in Biochemistry, University of Mysore, Mysore-570006, India

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Faiyaz Ahmed,

Corresponding Author

Faiyaz Ahmed

[email protected]

Department of Studies in Food Science and Nutrition, University of Mysore, Mysore-570006, India

Faiyaz Ahmed, Department of Studies in Food Science and Nutrition, University of Mysore, Mysore-570006, India

Bannikuppe S. Vishwanath, Department of Studies in Biochemistry, University of Mysore, Mysore-570006, India

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Jalahalli M. Siddesha,

Jalahalli M. Siddesha

Department of Studies in Biochemistry, University of Mysore, Mysore-570006, India

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Asna Urooj,

Asna Urooj

Department of Studies in Food Science and Nutrition, University of Mysore, Mysore-570006, India

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Bannikuppe S. Vishwanath,

Corresponding Author

Bannikuppe S. Vishwanath

[email protected]

Department of Studies in Biochemistry, University of Mysore, Mysore-570006, India

Faiyaz Ahmed, Department of Studies in Food Science and Nutrition, University of Mysore, Mysore-570006, India

Bannikuppe S. Vishwanath, Department of Studies in Biochemistry, University of Mysore, Mysore-570006, India

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First published: 22 November 2010

https://doi.org/10.1002/ptr.3205

Citations: 20

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Abstract

The present study evaluated the radical scavenging and angiotensin converting enzyme (ACE) inhibitory activity of cold and hot aqueous extracts of Ficus racemosa (Moraceae) stem bark. The extracts were standardized using HPLC. Radical scavenging activity was determined using 1,1-diphenyl-2-picrylhydrazyl radical and angiotensin converting enzyme inhibitory activity using rabbit lung and partially purified porcine kidney ACE. HPLC profiles of cold aqueous extract (FRC) showed the presence of bergenin, an isocoumarin, while hot aqueous extract (FRH) was found to contain ferulic acid, kaempferol and coumarin in addition to bergenin. FRH showed significantly higher (p ≤ 0.01) radical scavenging activity than FRC and butylated hydroxytoluene (BHT), consequently resulting in a significantly lower (p ≤ 0.01) IC₅₀ value than FRC and BHT. Both the extracts exhibited a dose dependent inhibition of porcine kidney and rabbit lung ACE. FRH showed significantly higher (p ≤ 0.01) activity than FRC with lower IC₅₀ values of 1.36 and 1.91 μg/mL respectively, for porcine kidney and rabbit lung ACE, compared with those of FRC (128 and 291 μg/mL). Further, a significant correlation (r = 0.893; p ≤ 0.05) was observed between radical scavenging activity and ACE-inhibitory activity. This is the first report on the ACE-inhibitory activity of F. racemosa stem bark suggesting its potential to be utilized as a therapeutic alternative for hypertension. Copyright © 2010 John Wiley & Sons, Ltd.

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Volume24, Issue12

December 2010

Pages 1839-1843

Radical scavenging and angiotensin converting enzyme inhibitory activities of standardized extracts of Ficus racemosa stem bark

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Radical scavenging and angiotensin converting enzyme inhibitory activities of standardized extracts of Ficus racemosa stem bark

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