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Endogenous phosphorylation in dunning prostate tumors of rats treated with LH-RH analogues

Georges Cehovic

Endocrine and Polypeptide Laboratories, Veterans Administration Medical Center, and Tulane University School of Medicine, New Orleans

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Tommie W. Redding,

Tommie W. Redding

Endocrine and Polypeptide Laboratories, Veterans Administration Medical Center, and Tulane University School of Medicine, New Orleans

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Marion T. Hierowski,

Marion T. Hierowski

Endocrine and Polypeptide Laboratories, Veterans Administration Medical Center, and Tulane University School of Medicine, New Orleans

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Dr. Andrew V. Schally,

Corresponding Author

Dr. Andrew V. Schally

Endocrine and Polypeptide Laboratories, Veterans Administration Medical Center, and Tulane University School of Medicine, New Orleans

151, Research Bldg 3, Veterans Administration Medical Center, 1601 Perdido St, New Orleans, LA 70146Search for more papers by this author

Georges Cehovic,

Georges Cehovic

Endocrine and Polypeptide Laboratories, Veterans Administration Medical Center, and Tulane University School of Medicine, New Orleans

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Tommie W. Redding,

Tommie W. Redding

Endocrine and Polypeptide Laboratories, Veterans Administration Medical Center, and Tulane University School of Medicine, New Orleans

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Marion T. Hierowski,

Marion T. Hierowski

Endocrine and Polypeptide Laboratories, Veterans Administration Medical Center, and Tulane University School of Medicine, New Orleans

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Dr. Andrew V. Schally,

Corresponding Author

Dr. Andrew V. Schally

Endocrine and Polypeptide Laboratories, Veterans Administration Medical Center, and Tulane University School of Medicine, New Orleans

151, Research Bldg 3, Veterans Administration Medical Center, 1601 Perdido St, New Orleans, LA 70146Search for more papers by this author

First published: 1984

https://doi.org/10.1002/pros.2990050606

Citations: 1

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Abstract

We investigated phosphorylation in Dunning R-3327H prostate tumor tissue of untreated rats, and rats treated with the agonist D-Trp⁶-LH-RH and antagonist N-Ac-D-p-Cl-Phe^1,2, D-Trp³, D-Arg⁶, D-Ala¹⁰-LH-RH. The total phosphorylation was significantly higher in Dunning tumors than in normal ventral and dorsal prostate. Incorporation of ³²P into tumor tissue of rats treated with D-Trp⁶-LH-RH was significantly lower than in tumors from untreated animals. The tumor regression produced by LH-RH agonist appeared to be linked with changes in the pattern of tumor protein phosphorylation. Although inhibition of tumor growth also occurred after administration of the LH-RH antagonist, no significant changes in phosphorylation were observed. The dissimilarity of effects of the agonists and the antagonists on protein phosphorylation in rat prostate tumors may be related to the differences in the mechanisms of action of these two types of LH-RH analogues.

References

1 Ahmed K: Studies on nuclear phosphoproteins of rat ventral prostate: Incorporation of ³²P from [gamma³²P] ATP. Biochim Biophys Acta 243: 38–48, 1971.
10.1016/0005-2795(71)90034-1
CAS Web of Science® Google Scholar
2 Cho-Chang YS, Redler BH: Dibutyryl cyclic AMP mimics ovariectomy: Nuclear protein phosphorylation in mammary tumor regression. Science 197: 272–275, 1977.
10.1126/science.195337
PubMed Web of Science® Google Scholar
3 Redding TW, Schally AV: Inhibition of prostate tumor growth in two rat models by chronic administration of D-Trp⁶ analogue of luteinizing hormone-releasing hormone. Proc Natl Acad Sci USA 78: 6509–6512, 1981.
10.1073/pnas.78.10.6509
CAS PubMed Web of Science® Google Scholar
4 Redding TW, Coy DH, Schally AV: Prostate carcinoma tumor size in rats decreases after administration of antagonists of luteinizing hormone-releasing hormone. Proc Natl Acad Sci USA 79: 1273–1276, 1982.
10.1073/pnas.79.4.1273
CAS PubMed Web of Science® Google Scholar
5 Schally AV, Redding TW, Comaru-Schally AM: Inhibition of prostate tumors by agonistic and antagonistic analogs of LH-RH. The Prostate 4: 545–552, 1983.
10.1002/pros.2990040602
CAS PubMed Web of Science® Google Scholar
6 Hierowski MT, Altamirano P, Redding TW, Schally AV: The presence of LHRH-like receptors in Dunning R 3327H prostate tumors. FEBS Lett 153: 92–96, 1983.
10.1016/0014-5793(83)80881-3
Web of Science® Google Scholar
7 Wiegant VM, Zwiers H, Gispen WH: Neuropeptides and brain cAMP and phosphoproteins. Pharmacol Ther 12: 463–490, 1981.
10.1016/0163-7258(81)90093-0
CAS PubMed Web of Science® Google Scholar
8 Flockhart DA, Corbin JD: Regulatory mechanisms in the control of protein kinases. CR Crit Rev Biochem 133–186, 1982.
Google Scholar
9 Cho-Chung YS: Cyclic AMP and its receptor protein in tumor growth regulation in vivo. J Cyclic Nucleotide Res 6 (3): 163–177, 1980.
CAS PubMed Web of Science® Google Scholar
10 Cehovic G: Etude de la présence dans le placenta humain de nouveaux facteurs. Mise au point d'une nouvelle méthode de dosage. C R Acad Sciences, Série III 293: 147–154, 1981.
CAS PubMed Web of Science® Google Scholar
11 Rudolph SA, Krueger BR: Endogenous protein phosphorylation and desphosphorylation. Adv Cyclic Nuceotide Res 10: 107–133, 1979.
CAS PubMed Google Scholar
12 Studier FW: Analysis of bacteriophage T7 early RNA's and proteins on slab gels. J Mol Biol 79: 237–248, 1973.
10.1016/0022-2836(73)90003-X
CAS PubMed Web of Science® Google Scholar
13 Dahlberg E, Snochowski M, Gustafsson JA: Comparison of the R-3327H rat prostatic adenocarcinoma to human benign prostatic hyperplasia and metastatic carcinoma of the prostate with regard to steroid hormone receptors. The Prostate 1: 61–70, 1980.
10.1002/pros.2990010109
CAS PubMed Google Scholar
14 Hierowski MT, Cehovic G, Redding TW, Schally AV: ³²P incorporation from (γ ³²-P)ATP into nuclear protein in Dunning prostate tumors of rats treated with D-Trp⁶-LH-RH. The Endocrine Meeting, Abstract 413, San Antonio, Texas, June 1983.
Google Scholar
15 Tolis G, Ackman D, Stellos A, Mehta A, Labrie F, Fazekas ATA, Comaru-Schally AM, Schally AV: Tumor growth inhibition in patients with prostatic carcinoma treated with luteinizing hormone-releasing hormone agonists. Proc Natl Acad Sci USA 79: 1658–1662, 1982.
10.1073/pnas.79.5.1658
CAS PubMed Web of Science® Google Scholar
16 Borgmann V, Hardt W, Schmidt-Gollwitzer M, Adenauer H, Nagel R: Sustained suppression of testosterone production by the luteinizing hormone-releasing hormone agonist buserelin in patients with advanced prostate carcinoma. Lancet 1: 1097–1099, 1982.
10.1016/S0140-6736(82)92279-6
CAS PubMed Web of Science® Google Scholar
17 Labrie F, Dupont A, Belanger A, Cusan L, Lacourciere Y, Monfette G, Laberge JG, Emond JP, Fazekas ATA, Raynaud JP, Husson JM: New hormonal therapy in prostatic carcinoma: Combined treatment with an LH-RH agonist and an antiandrogen. J. Clin Invest Med 5: 267–275, 1982.
CAS PubMed Web of Science® Google Scholar
18 Waxman JH, Wass JAH, Hendry WF, Whitfield HN, Besser GM, Malpas JS, Oliver RTD: Treatment with gonadotrophin releasing hormone analogue in advanced prostatic cancer. Br Med J 286: 1309–1312, 1983.
10.1136/bmj.286.6374.1309
CAS PubMed Web of Science® Google Scholar
19 Allen JM, O'Shea JP, Mashiter K, Williams G, Bloom SR: Advanced carcinoma of the prostate: Treatment with a gonadotrophin releasing hormone agonist. Br Med J 286: 1607–1609, 1983.
10.1136/bmj.286.6378.1607
CAS PubMed Web of Science® Google Scholar
20 Walker KJ, Nicholson RI, Turkes AO, Turkes A, Robinson M, Crispin Z, Dris S: Therapeutic potential of the LHRH agonist ICI 118630, in the treatment of advanced prostatic carcinoma. Lancet 2: 413–415, 1983.
10.1016/S0140-6736(83)90386-0
CAS PubMed Web of Science® Google Scholar
21 Corbin A: From contraception to cancer: A review of the therapeutic applications of LHRH analogs as antitumor agents. Yale J Biol Med 55: 27–47, 1982.
CAS PubMed Web of Science® Google Scholar

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Volume5, Issue6

1984

Pages 605-611

Endogenous phosphorylation in dunning prostate tumors of rats treated with LH-RH analogues

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Endogenous phosphorylation in dunning prostate tumors of rats treated with LH-RH analogues

Abstract

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