Volume 22, Issue 2 pp. 51-53
Biophotonics
Free Access

Unveiling cell secrets with lasers

Ultrafast lasers enable rapid cancer diagnosis with simultaneous two and three-photon imaging

Oliver Prochnow

Corresponding Author

Oliver Prochnow

VALO Innovations GmbH, Hollerithallee 17, 30419 Hannover, Germany

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Laura van Huizen

Laura van Huizen

Flash Pathology B.V., De Boelelaan 1085, 1081HV Amsterdam, The Netherlands

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First published: 16 June 2025

Abstract

A new generation of compact ultrafast laser systems capable of simultaneous two and three-photon microscopy – including two-photon fluorescence (2PF), second harmonic generation (SHG), three-photon fluorescence (3PF), and third harmonic generation (THG) imaging – offer exciting potential to accelerate biomedical research and clinical diagnostics.

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