Normal and dystrophic hamster myoblast and fibroblast growth in culture
Henry J. Klamut BSc
Department of biochemistry, Health Sciences Center, University of Western Ontario, London, Ontario, Canada
Search for more papers by this authorChao-Hsiung Lin PhD
Department of biochemistry, Health Sciences Center, University of Western Ontario, London, Ontario, Canada
Search for more papers by this authorCorresponding Author
Dr. Kenneth P. Strickland PhD
Department of biochemistry, Health Sciences Center, University of Western Ontario, London, Ontario, Canada
Strickland at the Department of Biochemistry, Health Sciences Center, University of Western Ontario, London, Ontario, Canada N6A 5C1Search for more papers by this authorHenry J. Klamut BSc
Department of biochemistry, Health Sciences Center, University of Western Ontario, London, Ontario, Canada
Search for more papers by this authorChao-Hsiung Lin PhD
Department of biochemistry, Health Sciences Center, University of Western Ontario, London, Ontario, Canada
Search for more papers by this authorCorresponding Author
Dr. Kenneth P. Strickland PhD
Department of biochemistry, Health Sciences Center, University of Western Ontario, London, Ontario, Canada
Strickland at the Department of Biochemistry, Health Sciences Center, University of Western Ontario, London, Ontario, Canada N6A 5C1Search for more papers by this authorAbstract
Normal and dystrophic hamster myoblasts and fibroblasts were compared for characteristic indicators of growth and differentiation. Clonal analysis of myoblast cultures indicated that 80% of colonies judged to be fusion-competent had differentiated. Dystrophic myoblasts were identical to normal in terms of their morphology, fusion potential (81.4%), and myokinase activity (59.6–49.1 mU/mg at 2–7 days), but displayed a significantly higher plating efficiency (normal: 52.6%; dystrophic: 82.1%), a longer doubling time (normal: 21.7 hours; dystrophic: 33.3 hours), and a lower day-7 creatine kinase activity (normal: 60.7 mU/mg; dystrophic: 41.3 mU/mg). Dystrophic fibroblasts were indistinguishable from normal ones in terms of their morphology, plating efficiency (90.4%), and doubling time (32.5 hours), but displayed a significantly lower day-2 creatine kinase activity (normal 58.3 mU/mg; dystrophic: 35.8 mU/mg) and day-7 myokinase activity (normal: 52.7 mU/mg; dystrophic: 39.9 mU/mg). The results are suggestive of an early and differential expression of the primary defect in dystrophic hamster myoblasts and fibroblasts in culture.
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