Experimental muscle injury: Correlation between ultrasound and histological findings
Fernando JimÉnez-Díaz MD, PhD
Laboratory of Performance and Sports Readaptation, Faculty of Sport Sciences, University of Castilla-La Mancha, Spain
Search for more papers by this authorIgnacio Jimena MD, PhD
Department of Morphological Sciences, Histology Section, Faculty of Medicine, University of Córdoba, Maimónides Institute for Biomedical Research, Avenida Menéndez Pidal s/n, Córdoba 14071, Spain
Search for more papers by this authorEvelio Luque MD, PhD
Department of Morphological Sciences, Histology Section, Faculty of Medicine, University of Córdoba, Maimónides Institute for Biomedical Research, Avenida Menéndez Pidal s/n, Córdoba 14071, Spain
Search for more papers by this authorSusana MendizÁbal PhD
Laboratory of Performance and Sports Readaptation, Faculty of Sport Sciences, University of Castilla-La Mancha, Spain
Search for more papers by this authorAntonio Bouffard MD
Detroit Medical Center, Sports Medicine Institute, Detroit, Michigan, USA
Search for more papers by this authorLuis JimÉnez-Reina MD, PhD
Department of Morphological Sciences, Histology Section, Faculty of Medicine, University of Córdoba, Maimónides Institute for Biomedical Research, Avenida Menéndez Pidal s/n, Córdoba 14071, Spain
Search for more papers by this authorCorresponding Author
JosÉ PeÑa MD, PhD
Department of Morphological Sciences, Histology Section, Faculty of Medicine, University of Córdoba, Maimónides Institute for Biomedical Research, Avenida Menéndez Pidal s/n, Córdoba 14071, Spain
Department of Morphological Sciences, Histology Section, Faculty of Medicine, University of Córdoba, Maimónides Institute for Biomedical Research, Avenida Menéndez Pidal s/n, Córdoba 14071, SpainSearch for more papers by this authorFernando JimÉnez-Díaz MD, PhD
Laboratory of Performance and Sports Readaptation, Faculty of Sport Sciences, University of Castilla-La Mancha, Spain
Search for more papers by this authorIgnacio Jimena MD, PhD
Department of Morphological Sciences, Histology Section, Faculty of Medicine, University of Córdoba, Maimónides Institute for Biomedical Research, Avenida Menéndez Pidal s/n, Córdoba 14071, Spain
Search for more papers by this authorEvelio Luque MD, PhD
Department of Morphological Sciences, Histology Section, Faculty of Medicine, University of Córdoba, Maimónides Institute for Biomedical Research, Avenida Menéndez Pidal s/n, Córdoba 14071, Spain
Search for more papers by this authorSusana MendizÁbal PhD
Laboratory of Performance and Sports Readaptation, Faculty of Sport Sciences, University of Castilla-La Mancha, Spain
Search for more papers by this authorAntonio Bouffard MD
Detroit Medical Center, Sports Medicine Institute, Detroit, Michigan, USA
Search for more papers by this authorLuis JimÉnez-Reina MD, PhD
Department of Morphological Sciences, Histology Section, Faculty of Medicine, University of Córdoba, Maimónides Institute for Biomedical Research, Avenida Menéndez Pidal s/n, Córdoba 14071, Spain
Search for more papers by this authorCorresponding Author
JosÉ PeÑa MD, PhD
Department of Morphological Sciences, Histology Section, Faculty of Medicine, University of Córdoba, Maimónides Institute for Biomedical Research, Avenida Menéndez Pidal s/n, Córdoba 14071, Spain
Department of Morphological Sciences, Histology Section, Faculty of Medicine, University of Córdoba, Maimónides Institute for Biomedical Research, Avenida Menéndez Pidal s/n, Córdoba 14071, SpainSearch for more papers by this authorAbstract
Introduction:
In this study we correlated ultrasound findings with histological changes taking place during experimentally induced degeneration–regeneration in rat skeletal muscle.
Methods:
Gastrocnemius muscles were injected with mepivacaine, and the progress of the muscle injury was monitored by ultrasound from day 1 to day 20. Muscles were extracted on the same days for histological examination.
Results:
The degenerative phase was characterized by increased echogenicity in the injured area; thereafter, echogenicity gradually diminished during the regenerative phase, attaining normal levels by 20 days postinjection. By this stage, histological examination revealed that regeneration was complete. The heteroechoic texture observed from day 4 to day 10 appeared to reflect the coexistence of degenerative and regenerative processes.
Conclusions:
The results suggest that the degenerative and regenerative phases of muscle injury may be distinguished sonographically through differences in echogenicity and echotexture and, using Doppler ultrasound, differences in the degree of vascularization. Muscle Nerve, 2012
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