Exercise training improves the soleus muscle morphology in experimental diabetic nerve regeneration
Tais Malysz PhD
Programa de Pós-Graduação em Neurociências, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul, RS, Brasil
Laboratório de Histofisiologia Comparada, Departamento de Ciências Morfológicas, Instituto de Ciâncias Básicas da Saúde, Universidade Federal do Rio Grande do Sul, Sarmento Leite 500, CEP 90050-170, Porto Alegre, RS, Brasil
Search for more papers by this authorJocemar Ilha PhD
Programa de Pós-Graduação em Neurociências, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul, RS, Brasil
Laboratório de Histofisiologia Comparada, Departamento de Ciências Morfológicas, Instituto de Ciâncias Básicas da Saúde, Universidade Federal do Rio Grande do Sul, Sarmento Leite 500, CEP 90050-170, Porto Alegre, RS, Brasil
Search for more papers by this authorPatrícia Severo do Nascimento MS
Programa de Pós-Graduação em Neurociências, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul, RS, Brasil
Laboratório de Histofisiologia Comparada, Departamento de Ciências Morfológicas, Instituto de Ciâncias Básicas da Saúde, Universidade Federal do Rio Grande do Sul, Sarmento Leite 500, CEP 90050-170, Porto Alegre, RS, Brasil
Search for more papers by this authorMaria Cristina Faccioni-Heuser PhD
Programa de Pós-Graduação em Neurociências, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul, RS, Brasil
Laboratório de Histofisiologia Comparada, Departamento de Ciências Morfológicas, Instituto de Ciâncias Básicas da Saúde, Universidade Federal do Rio Grande do Sul, Sarmento Leite 500, CEP 90050-170, Porto Alegre, RS, Brasil
Search for more papers by this authorKátia De Angelis PhD
Laboratório de Movimento Humano, Universidade de São Judas Tadeu, São Paulo, SP, Brasil
Search for more papers by this authorBeatriz D'agord Schaan MD, PhD
Programa de Pós-Graduação em Neurociências, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul, RS, Brasil
Serviço de Endocrinologia, Hospital de Clínicas de Porto Alegre, Universidade Federal do Rio Grande do Sul e Instituto de Cardiologia do Rio Grande do Sul, Fundação Universitária de Cardiologia Porto Alegre, RS, Brasil
Search for more papers by this authorCorresponding Author
Matilde Achaval MD, PhD
Programa de Pós-Graduação em Neurociências, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul, RS, Brasil
Laboratório de Histofisiologia Comparada, Departamento de Ciências Morfológicas, Instituto de Ciâncias Básicas da Saúde, Universidade Federal do Rio Grande do Sul, Sarmento Leite 500, CEP 90050-170, Porto Alegre, RS, Brasil
Programa de Pós-Graduação em Neurociências, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul, RS, BrasilSearch for more papers by this authorTais Malysz PhD
Programa de Pós-Graduação em Neurociências, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul, RS, Brasil
Laboratório de Histofisiologia Comparada, Departamento de Ciências Morfológicas, Instituto de Ciâncias Básicas da Saúde, Universidade Federal do Rio Grande do Sul, Sarmento Leite 500, CEP 90050-170, Porto Alegre, RS, Brasil
Search for more papers by this authorJocemar Ilha PhD
Programa de Pós-Graduação em Neurociências, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul, RS, Brasil
Laboratório de Histofisiologia Comparada, Departamento de Ciências Morfológicas, Instituto de Ciâncias Básicas da Saúde, Universidade Federal do Rio Grande do Sul, Sarmento Leite 500, CEP 90050-170, Porto Alegre, RS, Brasil
Search for more papers by this authorPatrícia Severo do Nascimento MS
Programa de Pós-Graduação em Neurociências, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul, RS, Brasil
Laboratório de Histofisiologia Comparada, Departamento de Ciências Morfológicas, Instituto de Ciâncias Básicas da Saúde, Universidade Federal do Rio Grande do Sul, Sarmento Leite 500, CEP 90050-170, Porto Alegre, RS, Brasil
Search for more papers by this authorMaria Cristina Faccioni-Heuser PhD
Programa de Pós-Graduação em Neurociências, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul, RS, Brasil
Laboratório de Histofisiologia Comparada, Departamento de Ciências Morfológicas, Instituto de Ciâncias Básicas da Saúde, Universidade Federal do Rio Grande do Sul, Sarmento Leite 500, CEP 90050-170, Porto Alegre, RS, Brasil
Search for more papers by this authorKátia De Angelis PhD
Laboratório de Movimento Humano, Universidade de São Judas Tadeu, São Paulo, SP, Brasil
Search for more papers by this authorBeatriz D'agord Schaan MD, PhD
Programa de Pós-Graduação em Neurociências, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul, RS, Brasil
Serviço de Endocrinologia, Hospital de Clínicas de Porto Alegre, Universidade Federal do Rio Grande do Sul e Instituto de Cardiologia do Rio Grande do Sul, Fundação Universitária de Cardiologia Porto Alegre, RS, Brasil
Search for more papers by this authorCorresponding Author
Matilde Achaval MD, PhD
Programa de Pós-Graduação em Neurociências, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul, RS, Brasil
Laboratório de Histofisiologia Comparada, Departamento de Ciências Morfológicas, Instituto de Ciâncias Básicas da Saúde, Universidade Federal do Rio Grande do Sul, Sarmento Leite 500, CEP 90050-170, Porto Alegre, RS, Brasil
Programa de Pós-Graduação em Neurociências, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul, RS, BrasilSearch for more papers by this authorAbstract
Introduction:
In this study we evaluate the effects of exercise training (10 weeks) on soleus muscle morphology in diabetic nerve regeneration after injury by sciatic nerve crush.
Methods:
Wistar rats were assigned to either a non-diabetic (n = 6), non-diabetic injured (n = 6), diabetic (n = 6), diabetic injured (DC; n = 9), or trained diabetic injured group (TDC; n = 7). Muscle transverse sections were used for morphometric and ultrastructural analyses.
Results:
Higher fiber density and smaller average myofiber area were observed in the DC and TDC (P < 0.05) groups compared with the other groups. This atrophic pattern was partially reversed in TDC. There was misalignment of the sarcomeres and structural alterations in the blood vessels, sarcolemma, nucleus, and mitochondria in the DC animals. The myofibers and blood vessels had a similar normal appearance in the TDC group. In addition, polyribosomes, rough sarcoplasmic reticulum, developed Golgi apparatus, and new myofibrils were observed.
Conclusions:
Sciatic nerve injury was found to promote soleus muscle atrophy and ultrastructural alterations in experimental diabetic nerve regeneration, which were partially reversed by exercise training. Muscle Nerve, 2011
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