Regulation of the calpain and ubiquitin-proteasome systems in a canine model of muscular dystrophy
Kristine M. Wadosky BS
Department of Pathology and Laboratory Medicine, University of North Carolina, Chapel Hill, North Carolina, USA
Department of Medicine and Pharmacology, University of North Carolina, Chapel Hill, North Carolina, USA
Search for more papers by this authorLuge Li BS
Department of Pathology and Laboratory Medicine, University of North Carolina, Chapel Hill, North Carolina, USA
Department of Medicine and Pharmacology, University of North Carolina, Chapel Hill, North Carolina, USA
Search for more papers by this authorJessica E. Rodríguez BS
Department of Pathology and Laboratory Medicine, University of North Carolina, Chapel Hill, North Carolina, USA
Department of Medicine and Pharmacology, University of North Carolina, Chapel Hill, North Carolina, USA
Search for more papers by this authorJin-na Min PhD
McAllister Heart Institute, University of North Carolina, Medical Biomolecular Research Building, 103 Mason Farm Road, Chapel Hill, North Carolina 27599-7525, USA
Department of Medicine and Pharmacology, University of North Carolina, Chapel Hill, North Carolina, USA
Search for more papers by this authorDan Bogan MS
Department of Pathology and Laboratory Medicine, University of North Carolina, Chapel Hill, North Carolina, USA
Department of Medicine and Pharmacology, University of North Carolina, Chapel Hill, North Carolina, USA
Search for more papers by this authorJason Gonzalez BS
Department of Exercise and Sports Science, University of North Carolina, Chapel Hill, North Carolina, USA
Department of Medicine and Pharmacology, University of North Carolina, Chapel Hill, North Carolina, USA
Search for more papers by this authorCam Patterson MD
McAllister Heart Institute, University of North Carolina, Medical Biomolecular Research Building, 103 Mason Farm Road, Chapel Hill, North Carolina 27599-7525, USA
Department of Medicine and Pharmacology, University of North Carolina, Chapel Hill, North Carolina, USA
Search for more papers by this authorJoe N. Kornegay DVM, PhD
Department of Pathology and Laboratory Medicine, University of North Carolina, Chapel Hill, North Carolina, USA
Department of Medicine and Pharmacology, University of North Carolina, Chapel Hill, North Carolina, USA
Search for more papers by this authorCorresponding Author
Monte Willis MD, PhD
Department of Pathology and Laboratory Medicine, University of North Carolina, Chapel Hill, North Carolina, USA
McAllister Heart Institute, University of North Carolina, Medical Biomolecular Research Building, 103 Mason Farm Road, Chapel Hill, North Carolina 27599-7525, USA
Department of Medicine and Pharmacology, University of North Carolina, Chapel Hill, North Carolina, USA
Department of Pathology and Laboratory Medicine, University of North Carolina, Chapel Hill, North Carolina, USASearch for more papers by this authorKristine M. Wadosky BS
Department of Pathology and Laboratory Medicine, University of North Carolina, Chapel Hill, North Carolina, USA
Department of Medicine and Pharmacology, University of North Carolina, Chapel Hill, North Carolina, USA
Search for more papers by this authorLuge Li BS
Department of Pathology and Laboratory Medicine, University of North Carolina, Chapel Hill, North Carolina, USA
Department of Medicine and Pharmacology, University of North Carolina, Chapel Hill, North Carolina, USA
Search for more papers by this authorJessica E. Rodríguez BS
Department of Pathology and Laboratory Medicine, University of North Carolina, Chapel Hill, North Carolina, USA
Department of Medicine and Pharmacology, University of North Carolina, Chapel Hill, North Carolina, USA
Search for more papers by this authorJin-na Min PhD
McAllister Heart Institute, University of North Carolina, Medical Biomolecular Research Building, 103 Mason Farm Road, Chapel Hill, North Carolina 27599-7525, USA
Department of Medicine and Pharmacology, University of North Carolina, Chapel Hill, North Carolina, USA
Search for more papers by this authorDan Bogan MS
Department of Pathology and Laboratory Medicine, University of North Carolina, Chapel Hill, North Carolina, USA
Department of Medicine and Pharmacology, University of North Carolina, Chapel Hill, North Carolina, USA
Search for more papers by this authorJason Gonzalez BS
Department of Exercise and Sports Science, University of North Carolina, Chapel Hill, North Carolina, USA
Department of Medicine and Pharmacology, University of North Carolina, Chapel Hill, North Carolina, USA
Search for more papers by this authorCam Patterson MD
McAllister Heart Institute, University of North Carolina, Medical Biomolecular Research Building, 103 Mason Farm Road, Chapel Hill, North Carolina 27599-7525, USA
Department of Medicine and Pharmacology, University of North Carolina, Chapel Hill, North Carolina, USA
Search for more papers by this authorJoe N. Kornegay DVM, PhD
Department of Pathology and Laboratory Medicine, University of North Carolina, Chapel Hill, North Carolina, USA
Department of Medicine and Pharmacology, University of North Carolina, Chapel Hill, North Carolina, USA
Search for more papers by this authorCorresponding Author
Monte Willis MD, PhD
Department of Pathology and Laboratory Medicine, University of North Carolina, Chapel Hill, North Carolina, USA
McAllister Heart Institute, University of North Carolina, Medical Biomolecular Research Building, 103 Mason Farm Road, Chapel Hill, North Carolina 27599-7525, USA
Department of Medicine and Pharmacology, University of North Carolina, Chapel Hill, North Carolina, USA
Department of Pathology and Laboratory Medicine, University of North Carolina, Chapel Hill, North Carolina, USASearch for more papers by this authorAbstract
Introduction: Previous studies have tested the hypothesis that calpain and/or proteasome inhibition is beneficial in Duchenne muscular dystrophy, based largely on evidence that calpain and proteasome activities are enhanced in the mdx mouse. Methods: mRNA expression of ubiquitin-proteasome and calpain system components were determined using real-time polymerase chain reaction in skeletal muscle and heart in the golden retriever muscular dystrophy model. Similarly, calpain 1 and 2 and proteasome activities were determined using fluorometric activity assays. Results: We found that less than half of the muscles tested had increases in proteasome activity, and only half had increased calpain activity. In addition, transcriptional regulation of the ubiquitin-proteasome system was most pronounced in the heart, where numerous components were significantly decreased. Conclusion: This study illustrates the diversity of expression and activities of the ubiquitin-proteasome and calpain systems, which may lead to unexpected consequences in response to pharmacological inhibition. Muscle Nerve, 2011
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