Quantitative magnetization transfer measured pool-size ratio reflects optic nerve myelin content in ex vivo mice
Xiawei Ou
Department of Radiology, Vanderbilt University Institute of Imaging Science, Nashville, Tennessee
Arkansas Children's Hospital, University of Arkansas for Medical Sciences, Little Rock, Arkansas
Search for more papers by this authorShu-Wei Sun
Department of Radiology, Washington University School of Medicine, St. Louis, Missouri
Search for more papers by this authorHsiao-Fang Liang
Department of Radiology, Washington University School of Medicine, St. Louis, Missouri
Search for more papers by this authorSheng-Kwei Song
Department of Radiology, Washington University School of Medicine, St. Louis, Missouri
Search for more papers by this authorCorresponding Author
Daniel F. Gochberg
Department of Radiology, Vanderbilt University Institute of Imaging Science, Nashville, Tennessee
Vanderbilt University Institute of Imaging Science, 1161 21st Avenue South, Medical Center North, AA-1105, Nashville, Tennessee 37232-2310===Search for more papers by this authorXiawei Ou
Department of Radiology, Vanderbilt University Institute of Imaging Science, Nashville, Tennessee
Arkansas Children's Hospital, University of Arkansas for Medical Sciences, Little Rock, Arkansas
Search for more papers by this authorShu-Wei Sun
Department of Radiology, Washington University School of Medicine, St. Louis, Missouri
Search for more papers by this authorHsiao-Fang Liang
Department of Radiology, Washington University School of Medicine, St. Louis, Missouri
Search for more papers by this authorSheng-Kwei Song
Department of Radiology, Washington University School of Medicine, St. Louis, Missouri
Search for more papers by this authorCorresponding Author
Daniel F. Gochberg
Department of Radiology, Vanderbilt University Institute of Imaging Science, Nashville, Tennessee
Vanderbilt University Institute of Imaging Science, 1161 21st Avenue South, Medical Center North, AA-1105, Nashville, Tennessee 37232-2310===Search for more papers by this authorAbstract
Optic nerves from mice that have undergone retinal ischemia were examined using a newly implemented quantitative magnetization transfer (qMT) technique. Previously published results indicate that the optic nerve from retinal ischemia mice suffered significant axon degeneration without detectable myelin injury at 3 days after reperfusion. At this time point, we acquired ex vivo qMT parameters from both shiverer mice (which have nearly no myelin) and control mice that have undergone retinal ischemia, and these qMT measures were compared with diffusion tensor imaging (DTI) results. Our findings suggests that the qMT estimated ratio of the pool sizes of the macromolecular and free water protons reflected the different myelin contents in the optic nerves between the shiverer and control mice. This pool size ratio was specific to myelin content only and was not significantly affected by the presence of axon injury in mouse optic nerve 3 days after retinal ischemia. Magn Reson Med 61:364–371, 2009. © 2009 Wiley-Liss, Inc.
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