Selective resonance suppression 1H-[13C] NMR spectroscopy with asymmetric adiabatic RF pulses
Lijing Xin
Laboratory of Functional and Metabolic Imaging (LIFMET), École Polytechnique Fédérale de Lausanne, Lausanne, Switzerland
Search for more papers by this authorHanne Frenkel
Laboratory of Functional and Metabolic Imaging (LIFMET), École Polytechnique Fédérale de Lausanne, Lausanne, Switzerland
Search for more papers by this authorVladimír Mlynárik
Laboratory of Functional and Metabolic Imaging (LIFMET), École Polytechnique Fédérale de Lausanne, Lausanne, Switzerland
Search for more papers by this authorFlorence D. Morgenthaler
Laboratory of Functional and Metabolic Imaging (LIFMET), École Polytechnique Fédérale de Lausanne, Lausanne, Switzerland
Search for more papers by this authorCorresponding Author
Rolf Gruetter
Laboratory of Functional and Metabolic Imaging (LIFMET), École Polytechnique Fédérale de Lausanne, Lausanne, Switzerland
Department of Radiology, University of Lausanne, Lausanne, Switzerland
Department of Radiology, University of Geneva, Geneva, Switzerland
École Polytechnique Fédérale de Lausanne, SB-LIFMET, CH F0 632, Station 6, CH-1015 Lausanne, Switzerland===Search for more papers by this authorLijing Xin
Laboratory of Functional and Metabolic Imaging (LIFMET), École Polytechnique Fédérale de Lausanne, Lausanne, Switzerland
Search for more papers by this authorHanne Frenkel
Laboratory of Functional and Metabolic Imaging (LIFMET), École Polytechnique Fédérale de Lausanne, Lausanne, Switzerland
Search for more papers by this authorVladimír Mlynárik
Laboratory of Functional and Metabolic Imaging (LIFMET), École Polytechnique Fédérale de Lausanne, Lausanne, Switzerland
Search for more papers by this authorFlorence D. Morgenthaler
Laboratory of Functional and Metabolic Imaging (LIFMET), École Polytechnique Fédérale de Lausanne, Lausanne, Switzerland
Search for more papers by this authorCorresponding Author
Rolf Gruetter
Laboratory of Functional and Metabolic Imaging (LIFMET), École Polytechnique Fédérale de Lausanne, Lausanne, Switzerland
Department of Radiology, University of Lausanne, Lausanne, Switzerland
Department of Radiology, University of Geneva, Geneva, Switzerland
École Polytechnique Fédérale de Lausanne, SB-LIFMET, CH F0 632, Station 6, CH-1015 Lausanne, Switzerland===Search for more papers by this authorAbstract
Despite obvious improvements in spectral resolution at high magnetic field, the detection of 13C labeling by 1H-[13C] NMR spectroscopy remains hampered by spectral overlap, such as in the spectral region of 1H resonances bound to C3 of glutamate (Glu) and glutamine (Gln), and C6 of N-acetylaspartate (NAA). The aim of this study was to develop, implement, and apply a novel 1H-[13C] NMR spectroscopic editing scheme, dubbed “selective Resonance suppression by Adiabatic Carbon Editing and Decoupling single-voxel STimulated Echo Acquisition Mode” (RACED-STEAM). The sequence is based on the application of two asymmetric narrow-transition-band adiabatic RF inversion pulses at the resonance frequency of the 13C coupled to the protons that need to be suppressed during the mixing time (TM) period, alternating the inversion band downfield and upfield from the 13C resonance on odd and even scans, respectively, thus suppressing the detection of 1H resonances bound to 13C within the transition band of the inversion pulse. The results demonstrate the efficient suppression of 1H resonances bound to C3 of Glu and Gln, and C4 of Glu, which allows the 1H resonances bound to C6 of NAA and C4 of Gln to be revealed. The measured time course of the resolved labeling into NAA C6 with the new scheme was consistent with the slow turnover of NAA. Magn Reson Med 61:260–266, 2009. © 2009 Wiley-Liss, Inc.
REFERENCES
- 1 Bluml S, Moreno A, Hwang JH, Ross BD. 1-(13)C glucose magnetic resonance spectroscopy of pediatric and adult brain disorders. NMR Biomed 2001; 14: 19–32.
- 2 Gruetter R, Novotny EJ, Boulware SD, Mason GF, Rothman DL, Shulman GI, Prichard JW, Shulman RG. Localized C-13 NMR-spectroscopy in the human brain of amino-acid labeling from D-[1-C-13]glucose. J Neurochem 1994; 63: 1377–1385.
- 3 Gruetter R, Ugurbil K, Seaquist ER. Steady-state cerebral glucose concentrations and transport in the human brain. J Neurochem 1998; 70: 397–408.
- 4 Lebon V, Petersen KF, Cline GW, Shen J, Mason GF, Dufour S, Behar KL, Shulman GI, Rothman DL. Astroglial contribution to brain energy metabolism in humans revealed by 13C nuclear magnetic resonance spectroscopy: elucidation of the dominant pathway for neurotransmitter glutamate repletion and measurement of astrocytic oxidative metabolism. J Neurosci 2002; 22: 1523–1531.
- 5 Mason GF, Rothman DL, Behar KL, Shulman RG. NMR determination of the TCA cycle rate and alpha-ketoglutarate glutamate exchange-rate in rat-brain. J Cereb Blood Flow Metab 1992; 12: 434–447.
- 6 Rothman DL, Novotny EJ, Shulman GI, Howseman AM, Petroff OAC, Mason G, Nixon T, Hanstock CC, Prichard JW, Shulman RG. H-1[C-13] NMR measurements of [4-C-13]glutamate turnover in human brain. Proc Natl Acad Sci USA 1992; 89: 9603–9606.
- 7 Sibson NR, Shen J, Mason GF, Rothman DL, Behar KL, Shulman RG. Functional energy metabolism: in vivo C-13-NMR spectroscopy evidence for coupling of cerebral glucose consumption and glutamatergic neuronal activity. Dev Neurosci 1998; 20: 321–330.
- 8 Gruetter R, Seaquist ER, Ugurbil K. A mathematical model of compartmentalized neurotransmitter metabolism in the human brain. Am J Physiol Endocrinol Metab 2001; 281: E100–E112.
- 9 Mason GF, Gruetter R, Rothman DL, Behar KL, Shulman RG, Novotny EJ. Simultaneous determination of the rates of the TCA cycle, glucose-utilization, alpha-ketoglutarate glutamate exchange, and glutamine synthesis in human brain by NMR. J Cereb Blood Flow Metab 1995; 15: 12–25.
- 10 Choi IY, Gruetter R. Dynamic or inert metabolism? Turnover of N-acetyl aspartate and glutathione from D-[1-C-13]glucose in the rat brain in vivo. J Neurochem 2004; 91: 778–787.
- 11 Henry PG, Tkac I, Gruetter R. H-1-localized broadband C-13 NMR spectroscopy of the rat brain in vivo at 9.4 T. Magn Reson Med 2003; 50: 684–692.
- 12 de Graaf RA, Brown PB, Mason GF, Rothman DL, Behar KL. Detection of [1,6-C-13]-glucose metabolism in rat brain by in vivo H-1[C-13]-NMR spectroscopy. Magn Reson Med 2003; 49: 37–46.
- 13 de Graaf RA, Mason GF, Patel AB, Behar KL, Rothman DL. In vivo 1H-[13C]-NMR spectroscopy of cerebral metabolism. NMR Biomed 2003; 16: 339–357.
- 14
Pfeuffer J,
Tkac I,
Choi IY,
Merkle H,
Ugurbil K,
Garwood M,
Gruetter R.
Localized in vivo 1H NMR detection of neurotransmitter labeling in rat brain during infusion of [1-13C] D-glucose.
Magn Reson Med
1999;
41:
1077–1083.
10.1002/(SICI)1522-2594(199906)41:6<1077::AID-MRM1>3.0.CO;2-# CAS PubMed Web of Science® Google Scholar
- 15 van Zijl PC, Chesnick AS, DesPres D, Moonen CT, Ruiz-Cabello J, van Gelderen P. In vivo proton spectroscopy and spectroscopic imaging of [1-13C]-glucose and its metabolic products. Magn Reson Med 1993; 30: 544–551.
- 16
Watanabe H,
Ishihara Y,
Okamoto K,
Oshio K,
Kanamatsu T,
Tsukada Y.
3D localized H-1-C-13 heteronuclear single-quantum coherence correlation spectroscopy in vivo.
Magn Reson Med
2000;
43:
200–210.
10.1002/(SICI)1522-2594(200002)43:2<200::AID-MRM6>3.0.CO;2-H CAS PubMed Web of Science® Google Scholar
- 17 Fitzpatrick SM, Hetherington HP, Behar KL, Shulman RG. The flux from glucose to glutamate in the rat-brain invivo as determined by H-1-observed, C-13-edited NMR-spectroscopy. J Cereb Blood Flow Metab 1990; 10: 170–179.
- 18 Rothman DL, Behar KL, Hetherington HP, Denhollander JA, Bendall MR, Petroff OAC, Shulman RG. H-1-observe C-13-decouple spectroscopic measurements of lactate and glutamate in the rat-brain invivo. Proc Natl Acad Sci USA 1985; 82: 1633–1637.
- 19
Gruetter R,
Tkac I.
Field mapping without reference scan using asymmetric echo-planar techniques.
Magn Reson Med
2000;
43:
319–323.
10.1002/(SICI)1522-2594(200002)43:2<319::AID-MRM22>3.0.CO;2-1 CAS PubMed Web of Science® Google Scholar
- 20 Provencher SW. Estimation of metabolite concentrations from localized in vivo proton NMR spectra. Magn Reson Med 1993; 30: 672–679.
- 21 Choi IY, Gruetter R. Dynamic or inert metabolism? Turnover of N-acetyl aspartate and glutathione from D-[1-13C]glucose in the rat brain in vivo. J Neurochem 2004; 91: 778–787.
- 22
Tkac I,
Starcuk Z,
Choi IY,
Gruetter R.
In vivo 1H NMR spectroscopy of rat brain at 1 ms echo time.
Magn Reson Med
1999;
41:
649–656.
10.1002/(SICI)1522-2594(199904)41:4<649::AID-MRM2>3.0.CO;2-G CAS PubMed Web of Science® Google Scholar
- 23 Hwang TL, van Zijl PC, Garwood M. Asymmetric adiabatic pulses for NH selection. J Magn Reson 1999; 138: 173–177.
- 24
Tannus A,
Garwood M.
Adiabatic pulses.
NMR Biomed
1997;
10:
423–434.
10.1002/(SICI)1099-1492(199712)10:8<423::AID-NBM488>3.0.CO;2-X CAS PubMed Web of Science® Google Scholar
- 25 Adriany G, Gruetter R. A half-volume coil for efficient proton decoupling in humans at 4 Tesla. J Magn Reson 1997; 125: 178–184.
- 26 Henry PG, Oz G, Provencher S, Gruetter R. Toward dynamic isotopomer analysis in the rat brain in vivo: automatic quantitation of 13C NMR spectra using LCModel. NMR Biomed 2003; 16: 400–412.
- 27 Henry PG, Tkac I, Gruetter R. 1H-localized broadband 13C NMR spectroscopy of the rat brain in vivo at 9.4 T. Magn Reson Med 2003; 50: 684–692.
- 28 Pfeuffer J, Tkac I, Provencher SW, Gruetter R. Toward an in vivo neurochemical profile: quantification of 18 metabolites in short-echo-time H NMR spectra of the rat brain. J Magn Reson 1999; 141: 104–120.
- 29 Clark JB. N-acetyl aspartate: a marker for neuronal loss or mitochondrial dysfunction. Dev Neurosci 1998; 20: 271–276.
- 30 Moreno A, Ross BD, Bluml S. Direct determination of the N-acetyl-L-aspartate synthesis rate in the human brain by (13)C MRS and [1-(13)C]glucose infusion. J Neurochem 2001; 77: 347–350.
- 31 Tyson RL, Sutherland GR. Labeling of N-acetylaspartate and N-acetylaspartylglutamate in rat neocortex, hippocampus and cerebellum from [1-13C]glucose. Neurosci Lett 1998; 251: 181–184.
- 32 Henry PG, Roussel R, Vaufrey F, Dautry C, Bloch G. Semiselective POCE NMR spectroscopy. Magn Reson Med 2000; 44: 395–400.
