Specific MR imaging of human lymphocytes by monoclonal antibody-guided dextran-magnetite particles
Jeff W. M. Bulte
Department of Clinical Immunology, Groningen, The Netherlands
Search for more papers by this authorYke Hoekstra
Department of Clinical Immunology, Groningen, The Netherlands
Search for more papers by this authorRichard L. Kamman
Department of Magnetic Resonance, Groningen, The Netherlands
Search for more papers by this authorRichard L. Magin
Department of Neurosurgery, Groningen, The Netherlands
Search for more papers by this authorAndrew G. Webb
Department of Neurosurgery, Groningen, The Netherlands
Histology and Cell Biology, University of Groningen, Oostersingel 59, Groningen, The Netherlands
Search for more papers by this authorRichard W. Briggs
Histology and Cell Biology, University of Groningen, Oostersingel 59, Groningen, The Netherlands
Search for more papers by this authorK. Gwan Go
Bioacoustics Research Laboratory, University of Illinois, Urbana, Illinois 61801
Search for more papers by this authorCaesar E. Hulstaert
Department of Radiology, University of Florida, Gainesville, Florida 32610
Search for more papers by this authorStefan Miltenyi
Miltenyi Biotec GmbH, Moitzfeld 60a, D-5060 Bergisch-Gladhach 1, Germany
Search for more papers by this authorT. Hauw The
Department of Clinical Immunology, Groningen, The Netherlands
Search for more papers by this authorLou De Leij
Department of Clinical Immunology, Groningen, The Netherlands
Search for more papers by this authorJeff W. M. Bulte
Department of Clinical Immunology, Groningen, The Netherlands
Search for more papers by this authorYke Hoekstra
Department of Clinical Immunology, Groningen, The Netherlands
Search for more papers by this authorRichard L. Kamman
Department of Magnetic Resonance, Groningen, The Netherlands
Search for more papers by this authorRichard L. Magin
Department of Neurosurgery, Groningen, The Netherlands
Search for more papers by this authorAndrew G. Webb
Department of Neurosurgery, Groningen, The Netherlands
Histology and Cell Biology, University of Groningen, Oostersingel 59, Groningen, The Netherlands
Search for more papers by this authorRichard W. Briggs
Histology and Cell Biology, University of Groningen, Oostersingel 59, Groningen, The Netherlands
Search for more papers by this authorK. Gwan Go
Bioacoustics Research Laboratory, University of Illinois, Urbana, Illinois 61801
Search for more papers by this authorCaesar E. Hulstaert
Department of Radiology, University of Florida, Gainesville, Florida 32610
Search for more papers by this authorStefan Miltenyi
Miltenyi Biotec GmbH, Moitzfeld 60a, D-5060 Bergisch-Gladhach 1, Germany
Search for more papers by this authorT. Hauw The
Department of Clinical Immunology, Groningen, The Netherlands
Search for more papers by this authorLou De Leij
Department of Clinical Immunology, Groningen, The Netherlands
Search for more papers by this authorAbstract
Human lymphocytes were labeled with biotinylated anti-lymphocyte-directed monoclonal antibodies, to which streptavidin and subsequently biotinylated dextran-magnetite particles were coupled. This labeling resulted in a strong and selective negative contrast enhancement of lymphocyte suspensions at 2.0 T, caused predominantly by the specific increase of R2 with a small but significant specific increase of R2. The R1 was found to decrease with increasing field strength. The immunolabeling procedure described here may be used for the selective signal depletion of target cells in MR imaging.
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