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Structural study of hyaluronic acid oligomers and their complexes with copper in water by NMR and IR and molecular dynamics calculations
Abstract
The hexasaccharide (trimer) and tetrasaccharide (dimer) oligomers of hyaluronic acid were investigated by 1H and 13C high resolution Nuclear Magnetic Resonance. The dynamic behavior of the molecules and their complexation with copper(II) were analysed by 1H NMR relaxation studies. A specific site for the complexation of the tetrasaccharide with Cu2+ was demonstrated by analysis of the paramagnetic effect of the metal on non-selective proton relaxation rates. A model for the complex involving two molecules of the tetrasaccharide is proposed.
