Liposomal Drug Deposits in Poly(Dopamine) Coatings: Effect of Their Composition, Cell Type, Uptake Pathway Considerations, and Shear Stress

Figure S1. a) Dissipation changes ΔD of crystals upon exposure to M^zw, M^zw/OL1(25), M^zw/ST1(25), M^zw/PL1(5,10), M^zwp, or M^zw/PL1(5,10) for 70 min changing the solution after 35 min. (ϕ: The solution was not changed within the 70 min.) b) Frequency change Δf of a crystal upon coating with PLL and M^zw/PL10 including the buffer washing steps. c) Dissipation changes ΔD of crystals upon exposure to M^zw/ST25 using different DA concentrations in the mixture for 70 min.

Figure S2. a) Representative brightfield microscopy images of myoblasts adhering to M^zw, M^zw/ST25 and M^zw/PL10 for 24 h. Scale bars are 10 µm. b) Photograph of the well plate containing the glass slides after coating with M^zw, M^zw/ST25 and M^zw/OL25, showing that the solution in the last case aggregated within the coating time.

Figure S3. Charged liposomes: Change in dissipation ΔD of crystals upon exposure to M^zw, Mⁿ¹⁽²⁾, M^p1(2) and M^p1(2)/ST25 using 2.5 mg/mL DA. Adsorption onto PLL was measured for M^zw, Mⁿ, and onto PLL, PLL/PMA and PLL/PMA_c for M^p.

Figure S4. A photograph of solutions containing freshly extruded L^p2/ST25 (left) and L^p1/ST25 (right). A pinkish color is observed, which is more pronounced for higher content of positively charges lipids in the liposomes.

Figure S5. Representative bright field images of macrophages adhered for 3 h to substrates coated with M^zw, Mⁿ¹⁽²⁾, or M^p1(2). Scale bars are 25 µm.

Figure S6. Representative bright field images of hepatocytes adhered 3 h to substrates coated with M^zw, Mⁿ¹⁽²⁾, or M^p1(2). Scale bars are 25 µm.

Figure S7. Representative bright field microscopy images of myoblasts cells adhered 3 h to substrates coated with M^zw, Mⁿ¹⁽²⁾, or M^p1(2). Scale bars are 25 µm

Figure S8. Representative bright field images of endothelial cells adhered for 3 h to substrates coated with M^zw, M^{n1 (2)}, or M^{p1 (2)}. Scale bars are 25 µm.

Figure S9. Representative CLSM images of macrophages adhered for 3 h to substrates coated with M^zw, Mⁿ¹⁽²⁾, or M^p1(2). Scale bars are 50 µm.

Figure S10. Representative CLSM images of hepatocytes adhered for 3 h to substrates coated with M^zw, Mⁿ¹⁽²⁾, or M^p1(2). Scale bars are 50 µm.

Figure S11. Representative CLSM images of myoblasts adhered for 3 h to substrates coated with M^zw, Mⁿ¹⁽²⁾, or M^p1(2). Scale bars are 50 µm.

Figure S12. Representative CLSM images of endothelial cells adhered for 3 h to substrates coated with Mⁿ¹⁽²⁾. Scale bars are 50 µm.

Figure S13. Cell viability of cells incubated for 3 h in the presence of inhibitors with concentrations 1x, 2x and 4x. Amil: Amiloride (x: 50 µM), Cp: Chlorpromazine (x: 10 µM), Fil: Filipin (x: 1 µg/mL)

Figure S14. Uptake pathway: CMF of myoblasts exposed to Lⁿ² for 3 h in the presence of different chemical inhibitors (Ami: Amiloride (200 µg/ml), Cp: chlorpromazine (10 µg/mL), Fil: filipin complex (1 µM)) for specific endocytosis uptake pathways are shown. Control experiments using untreated cells (C), cells with PBS (P) or PBS and DMSO (D) but without inhibitors are shown for comparison.

Figure S15. Mass spectra data of PL-DA (a) and ST-DA (b).

Table S1. Diameter and PDI of liposomes containing different lipophilic DA-conjugates.

Table S2. Diameter and PDI of liposomes containing different charged lipids and/or lipophilic DA-conjugates.