Enhanced flexor tendon healing through controlled delivery of PDGF-BB
Corresponding Author
Stavros Thomopoulos
Department of Orthopaedic Surgery, Washington University, St. Louis, Missouri
Department of Orthopaedic Surgery, Washington University, St. Louis, Missouri, Telephone: 314-362-8605; Fax: 314-362-0334Search for more papers by this authorRosalina Das
Department of Orthopaedic Surgery, Washington University, St. Louis, Missouri
Search for more papers by this authorMatthew J. Silva
Department of Orthopaedic Surgery, Washington University, St. Louis, Missouri
Search for more papers by this authorShelly Sakiyama-Elbert
Department of Biomedical Engineering, Washington University, St. Louis, Missouri
Search for more papers by this authorFrederick L. Harwood
Department of Orthopaedic Surgery, University of California San Diego, San Diego, California
Search for more papers by this authorEmmanouil Zampiakis
Department of Orthopaedic Surgery, Washington University, St. Louis, Missouri
Search for more papers by this authorH. Mike Kim
Department of Orthopaedic Surgery, Washington University, St. Louis, Missouri
Search for more papers by this authorDavid Amiel
Department of Orthopaedic Surgery, University of California San Diego, San Diego, California
Search for more papers by this authorRichard H. Gelberman
Department of Orthopaedic Surgery, Washington University, St. Louis, Missouri
Search for more papers by this authorCorresponding Author
Stavros Thomopoulos
Department of Orthopaedic Surgery, Washington University, St. Louis, Missouri
Department of Orthopaedic Surgery, Washington University, St. Louis, Missouri, Telephone: 314-362-8605; Fax: 314-362-0334Search for more papers by this authorRosalina Das
Department of Orthopaedic Surgery, Washington University, St. Louis, Missouri
Search for more papers by this authorMatthew J. Silva
Department of Orthopaedic Surgery, Washington University, St. Louis, Missouri
Search for more papers by this authorShelly Sakiyama-Elbert
Department of Biomedical Engineering, Washington University, St. Louis, Missouri
Search for more papers by this authorFrederick L. Harwood
Department of Orthopaedic Surgery, University of California San Diego, San Diego, California
Search for more papers by this authorEmmanouil Zampiakis
Department of Orthopaedic Surgery, Washington University, St. Louis, Missouri
Search for more papers by this authorH. Mike Kim
Department of Orthopaedic Surgery, Washington University, St. Louis, Missouri
Search for more papers by this authorDavid Amiel
Department of Orthopaedic Surgery, University of California San Diego, San Diego, California
Search for more papers by this authorRichard H. Gelberman
Department of Orthopaedic Surgery, Washington University, St. Louis, Missouri
Search for more papers by this authorAbstract
A fibrin/heparin-based delivery system was used to provide controlled delivery of platelet derived growth factor BB (PDGF-BB) in an animal model of intrasynovial flexor tendon repair. We hypothesized that PDGF-BB, administered in this manner, would stimulate cell proliferation and matrix remodeling, leading to improvements in the sutured tendon's functional and structural properties. Fifty-six flexor digitorum profundus tendons were injured and repaired in 28 dogs. Three groups were compared: (1) controlled delivery of PDGF-BB using a fibrin/heparin-based delivery system; (2) delivery system carrier control; and (3) repair- only control. The operated forelimbs were treated with controlled passive motion rehabilitation. The animals were euthanized at 7, 14, and 42 days, at which time the tendons were assessed using histologic (hyaluronic acid content, cellularity, and inflammation), biochemical (total DNA and reducible collagen crosslink levels), and biomechanical (gliding and tensile properties) assays. We found that cell activity (as determined by total DNA, collagen crosslink analyses, and hyaluronic acid content) was accelerated due to PDGF-BB at 14 days. Proximal interphalangeal joint rotation and tendon excursion (i.e., tendon gliding properties) were significantly higher for the PDGF-BB-treated tendons compared to the repair-alone tendons at 42 days. Improvements in tensile properties were not achieved, possibly due to suboptimal release kinetics or other factors. In conclusion, PDGF-BB treatment consistently improved the functional but not the structural properties of sutured intrasynovial tendons through 42 days following repair. © 2009 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res
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