An efficient protocol for isolating melanised chaetothyrialean anamorphic fungi associated with plant-ants
Corresponding Author
Mario X. Ruiz-González
Université de Toulouse, UPS, UMR5174 EDB (Laboratoire Evolution et Diversité Biologique), Toulouse, France
CNRS, UMR5174 EDB (Laboratoire Evolution et Diversité Biologique), Toulouse, France
Abiotic Stress Department, Integrative and Systems Biology Group. Instituto de Biología Molecular y Celular de Plantas, C.S.I.C.–U.P.V. C/Ingeniero Fausto Elio s/n., Valencia, Spain
Correspondence: Dr. M. X. Ruiz-González, Abiotic Stress Department, Integrative and Systems Biology Group. Instituto de Biología Molecular y Celular de Plantas, C.S.I.C. – U.P.V. C/Ingeniero Fausto Elio s/n. 46022 Valencia, Spain
E-mail:[email protected]
Phone: +34 963879934
Fax: +34 963877859
Search for more papers by this authorJérémie Lauth
Université des Antilles et de la Guyane; UMR Ecologie des forêts de Guyane, Campus Agronomique, Kourou, France
Search for more papers by this authorCéline Leroy
CNRS, UMR Ecologie des Forêts de Guyane, Campus Agronomique, Kourou, France
Search for more papers by this authorAlain Jauneau
CNRS, IFR40 Pôle de Biotechnologie Végétale, Plateforme d'imagerie cellulaire de Toulouse, Auzeville, Castanet-Tolosan, France
Search for more papers by this authorHervé Gryta
Université de Toulouse, UPS, UMR5174 EDB (Laboratoire Evolution et Diversité Biologique), Toulouse, France
CNRS, UMR5174 EDB (Laboratoire Evolution et Diversité Biologique), Toulouse, France
Search for more papers by this authorPatricia Jargeat
Université de Toulouse, UPS, UMR5174 EDB (Laboratoire Evolution et Diversité Biologique), Toulouse, France
CNRS, UMR5174 EDB (Laboratoire Evolution et Diversité Biologique), Toulouse, France
Search for more papers by this authorAlain Dejean
CNRS, UMR Ecologie des Forêts de Guyane, Campus Agronomique, Kourou, France
Université de Toulouse, ECOLAB, Toulouse, France
Search for more papers by this authorJérôme Orivel
CNRS, UMR Ecologie des Forêts de Guyane, Campus Agronomique, Kourou, France
Search for more papers by this authorCorresponding Author
Mario X. Ruiz-González
Université de Toulouse, UPS, UMR5174 EDB (Laboratoire Evolution et Diversité Biologique), Toulouse, France
CNRS, UMR5174 EDB (Laboratoire Evolution et Diversité Biologique), Toulouse, France
Abiotic Stress Department, Integrative and Systems Biology Group. Instituto de Biología Molecular y Celular de Plantas, C.S.I.C.–U.P.V. C/Ingeniero Fausto Elio s/n., Valencia, Spain
Correspondence: Dr. M. X. Ruiz-González, Abiotic Stress Department, Integrative and Systems Biology Group. Instituto de Biología Molecular y Celular de Plantas, C.S.I.C. – U.P.V. C/Ingeniero Fausto Elio s/n. 46022 Valencia, Spain
E-mail:[email protected]
Phone: +34 963879934
Fax: +34 963877859
Search for more papers by this authorJérémie Lauth
Université des Antilles et de la Guyane; UMR Ecologie des forêts de Guyane, Campus Agronomique, Kourou, France
Search for more papers by this authorCéline Leroy
CNRS, UMR Ecologie des Forêts de Guyane, Campus Agronomique, Kourou, France
Search for more papers by this authorAlain Jauneau
CNRS, IFR40 Pôle de Biotechnologie Végétale, Plateforme d'imagerie cellulaire de Toulouse, Auzeville, Castanet-Tolosan, France
Search for more papers by this authorHervé Gryta
Université de Toulouse, UPS, UMR5174 EDB (Laboratoire Evolution et Diversité Biologique), Toulouse, France
CNRS, UMR5174 EDB (Laboratoire Evolution et Diversité Biologique), Toulouse, France
Search for more papers by this authorPatricia Jargeat
Université de Toulouse, UPS, UMR5174 EDB (Laboratoire Evolution et Diversité Biologique), Toulouse, France
CNRS, UMR5174 EDB (Laboratoire Evolution et Diversité Biologique), Toulouse, France
Search for more papers by this authorAlain Dejean
CNRS, UMR Ecologie des Forêts de Guyane, Campus Agronomique, Kourou, France
Université de Toulouse, ECOLAB, Toulouse, France
Search for more papers by this authorJérôme Orivel
CNRS, UMR Ecologie des Forêts de Guyane, Campus Agronomique, Kourou, France
Search for more papers by this authorAbstract
Because of their ecological characteristics, slow growth rates and the presence of contaminants, Chaetothyriales fungi associated with structures built by tropical plant-ants can be difficult to isolate with standard procedures. Here, we describe an easy-to-use protocol for obtaining pure cultures by using cotton as a first substrate. We have further found by means of fluorescent stains that nuclei concentrate either in young hyphae or in the tips of the hyphae.
References
- 1 de Hoog, G.S., Zalar, P., Urzì, C., de Leo, F., Yurlova, N.A., Sterflinger, K., et al. 1999, Relationships of dothideaceous black yeasts and meristematic fungi base don 5.8.S and ITS2 rDNA sequence comparison. Stud. Mycol., 43, 31–37.
- 2 Gueidan, C., 2008, Chaetothyriales. Version 29 January 2008 (under construction). http://tolweb.org/Chaetothyriales/29305/2008.01.29 in The Tree of Life Web Project, http://tolweb.org/.
- 3 Gueidan, C., Ruibal Villaseñor, C., de Hoog, G.S., Gorbushina, A.A., et al., 2008, A rock-inhabiting ancestor for mutualistic and pathogen-rich fungal lineages. Stud. Mycol., 61, 111–119.
- 4 Hughes, S.J., 1976, Sooty moulds. Mycologia, 68, 693–820.
- 5 Defossez, E., Selosse, M.A., Dubois, M.P., Mondolot, L., et al., 2009, Ants-plants and fungi: a new threeway symbiosis. New Phytol., 182, 942–949.
- 6 Mayer, V.E., Voglmayr, H., 2009, Mycelial carton galleries of Azteca brevis (Formicidae) as a multi-species network. P.R. Soc. London, 276, 3265–3273.
- 7 Ruiz-González, M.X., Malé, P.-J.G., Leroy, C., Dejean, A., et al., 2011, Specific, non-nutritional association between an ascomycete fungus and Allomerus plant-ants. Biol. Lett., 7, 475–479.
- 8 Voglmayr, H., Mayer, V., Maschwitz, U., Moog, J., et al., 2011, The diversity of ant-associated black yeasts: insights into a newly discovered world of symbiotic interactions. Fungal Biol. 115, 1077–1091. DOI: 10.1016/j.funbio.2010.11.006.
- 9 Dejean, A., Solano, P.J., Ayroles, J., Corbara, B., Orivel, J., 2005, Arboreal ants build traps to capture prey. Nature, 434, 973.
- 10 Rao, P.S., Niederpruem, D.J., 1969, Carbohydrate metabolism during morphogenesis of Coprinus lagopus (sensu Buller). J. Bacteriol., 100, 1222–1228.
