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Susceptibility of a Vibrio alginolyticus rpoS mutant to environmental stresses and its expression of OMPs
Wang Shuxian,
Wei Jianteng,
Li Tianbao,
Corresponding Author
Wang Shuxian
Mariculture Institute of Shandong Province, Shandong Province Key Laboratory for Disease Control of Mariculture, Guizhou Road, Qingdao City, Shandong Province, China
Phone: +86 532 86511767, Fax: +86 532 86558191Search for more papers by this authorWei Jianteng
Institute of Oceanology, Chinese Academy of Sciences, Nanhai Road, Qingdao City, Shandong Province, China
Search for more papers by this authorLi Tianbao
Mariculture Institute of Shandong Province, Shandong Province Key Laboratory for Disease Control of Mariculture, Guizhou Road, Qingdao City, Shandong Province, China
Search for more papers by this authorWang Shuxian,
Wei Jianteng,
Li Tianbao,
Corresponding Author
Wang Shuxian
Mariculture Institute of Shandong Province, Shandong Province Key Laboratory for Disease Control of Mariculture, Guizhou Road, Qingdao City, Shandong Province, China
Phone: +86 532 86511767, Fax: +86 532 86558191Search for more papers by this authorWei Jianteng
Institute of Oceanology, Chinese Academy of Sciences, Nanhai Road, Qingdao City, Shandong Province, China
Search for more papers by this authorLi Tianbao
Mariculture Institute of Shandong Province, Shandong Province Key Laboratory for Disease Control of Mariculture, Guizhou Road, Qingdao City, Shandong Province, China
Search for more papers by this authorAbstract
Vibrio alginolyticus, one of the most important opportunistic pathogens, can be detected in human being and marine animals. Like other bacteria, V. alginolyticus is able to adapt to a variety of stressful environmental changes. The alternate sigma factor RpoS, which is a regulator during stationary phase, plays an important role in surviving under these stressful situations in many bacteria. Sequence analysis reveals a 990 bp open reading frame which is predicted to encode a 330-amino-acid protein with 68% to 96% overall identity to other reported sequences. To study the function of rpoS, the rpoS gene of V. alginolyticus VIB283 was cloned and an rpoS mutant was constructed by homologous recombination. Comparison of the study result of the wild type and the mutant showed that the mutant was more sensitive to stress conditions such as high osmolarity, oxidative stress, heat shock, and long-term starvation and that the LD50 of the mutant strain to the zebra fish was about 2.8 times as that of the control strain. In addition, the SDS-PAGE analysis indicated that the outer membrane proteins (OMPs) existed great differences. (© 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim)
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