Rapid investigation of hepatitis A virus outbreak by single strand conformation polymorphism analysis
Corresponding Author
Vincent Mackiewicz
Centre National de Référence du Virus de l'Hépatite A, Laboratoire de Virologie, Hôpital Paul Brousse, Assistance Publique-Hôpitaux de Paris, Villejuif, France
Laboratoire de Virologie, Hôpital Paul Brousse, 12 avenue Paul Vaillant-Couturier, 94804 Villejuif, France.===Search for more papers by this authorAnne-Marie Roque-Afonso
Centre National de Référence du Virus de l'Hépatite A, Laboratoire de Virologie, Hôpital Paul Brousse, Assistance Publique-Hôpitaux de Paris, Villejuif, France
Search for more papers by this authorEric Marchadier
Centre National de Référence du Virus de l'Hépatite A, Laboratoire de Virologie, Hôpital Paul Brousse, Assistance Publique-Hôpitaux de Paris, Villejuif, France
Search for more papers by this authorElisabeth Nicand
Laboratoire de Biologie Clinique, Hôpital d'Instruction des Armées Val de Grâce, Paris, France
Search for more papers by this authorLamia Fki-Berrajah
Service de Biologie, Hôpital Habib Bourguiba, Sfax, Tunisie
Search for more papers by this authorElisabeth Dussaix
Centre National de Référence du Virus de l'Hépatite A, Laboratoire de Virologie, Hôpital Paul Brousse, Assistance Publique-Hôpitaux de Paris, Villejuif, France
Search for more papers by this authorCorresponding Author
Vincent Mackiewicz
Centre National de Référence du Virus de l'Hépatite A, Laboratoire de Virologie, Hôpital Paul Brousse, Assistance Publique-Hôpitaux de Paris, Villejuif, France
Laboratoire de Virologie, Hôpital Paul Brousse, 12 avenue Paul Vaillant-Couturier, 94804 Villejuif, France.===Search for more papers by this authorAnne-Marie Roque-Afonso
Centre National de Référence du Virus de l'Hépatite A, Laboratoire de Virologie, Hôpital Paul Brousse, Assistance Publique-Hôpitaux de Paris, Villejuif, France
Search for more papers by this authorEric Marchadier
Centre National de Référence du Virus de l'Hépatite A, Laboratoire de Virologie, Hôpital Paul Brousse, Assistance Publique-Hôpitaux de Paris, Villejuif, France
Search for more papers by this authorElisabeth Nicand
Laboratoire de Biologie Clinique, Hôpital d'Instruction des Armées Val de Grâce, Paris, France
Search for more papers by this authorLamia Fki-Berrajah
Service de Biologie, Hôpital Habib Bourguiba, Sfax, Tunisie
Search for more papers by this authorElisabeth Dussaix
Centre National de Référence du Virus de l'Hépatite A, Laboratoire de Virologie, Hôpital Paul Brousse, Assistance Publique-Hôpitaux de Paris, Villejuif, France
Search for more papers by this authorAbstract
Investigation of hepatitis A virus (HAV) outbreaks often implies nucleotide sequence analysis. As an alternative method for the identification of related strains, single strand conformation polymorphism method (SSCP) was compared to sequence analysis. Twenty-three strains from sporadic and outbreak cases were studied retrospectively. SSCP, sequence identity and phylogenetic analyses were conducted on a 267 bp fragment of the VP1-2A variable region. The results of SSCP pattern comparison and sequence identity were highly correlated (r = 0.92, P < 0.001). If SSCP showed similar patterns, the VP1-2A fragments had a high and significant probability to have a sequence identity over 99.6%. Results were concordant for outbreak strains. The only discordant result concerned a cluster of three sporadic cases evidenced by phylogenetic analysis while SSCP showed similar patterns for only two of these three cases. A prospective SSCP analysis of a recent HAV outbreak confirmed the reliability of this technique. SSCP may thus provide a rapid and cost-effective tool for preliminary investigation of HAV outbreaks, before undertaking exhaustive nucleotide sequence analysis. J. Med. Virol. 76:271–278, 2005. © 2005 Wiley-Liss, Inc.
REFERENCES
- Apaire-Marchais V, Robertson BH, Aubineau-Ferre V, Le Roux MG, Leveque F, Schwartzbrod L, Billaudel S. 1995. Direct sequencing of hepatitis A virus strains isolated during an epidemic in France. Appl Environ Microbiol 61: 3977–3980.
- Arauz-Ruiz P, Sundqvist L, Garcia Z, Taylor L, Visona K, Norder H, Magnius LO. 2001. Presumed common source outbreaks of hepatitis A in an endemic area confirmed by limited sequencing within the VP1 region. J Med Virol 65: 449–456.
- F Blaine Hollinger, JR Ticehurst, editors. 1996. Hepatitis A virus, 3rd edn. Philadelphia: Lippincott-Raven Publishers. pp 735–785.
- Bower WA, Nainan OV, Han X, Margolis HS. 2000. Duration of viremia in hepatitis A virus infection. J Infect Dis 182: 12–17.
- Chironna M, Grottola A, Lanave C, Villa E, Barbuti S, Quarto M. 2003. Genetic analysis of HAV strains recovered from patients with acute hepatitis from Southern Italy. J Med Virol 70: 343–349.
- Costa-Mattioli M, Ferre V, Monpoeho S, Garcia L, Colina R, Billaudel S, Vega I, Perez-Bercoff R, Cristina J. 2001a. Genetic variability of hepatitis A virus in South America reveals heterogeneity and co-circulation during epidemic outbreaks. J Gen Virol 82: 2647–2652.
- Costa-Mattioli M, Monpoeho S, Schvoerer C, Besse B, Aleman MH, Billaudel S, Cristina J, Ferre V. 2001b. Genetic analysis of hepatitis A virus outbreak in France confirms the co-circulation of subgenotypes Ia, Ib and reveals a new genetic lineage. J Med Virol 65: 233–240.
- Cotter SM, Sansom S, Long T, Koch E, Kellerman S, Smith F, Averhoff F, Bell BP. 2003. Outbreak of hepatitis A among men who have sex with men: Implications for hepatitis A vaccination strategies. J Infect Dis 187: 1235–1240.
-
Dal-Re R,
Garcia-Corbeira P,
Garcia-de-Lomas J.
2000.
A large percentage of the Spanish population under 30 years of age is not protected against hepatitis A.
J Med Virol
60:
363–366.
10.1002/(SICI)1096-9071(200004)60:4<363::AID-JMV1>3.0.CO;2-L CAS PubMed Web of Science® Google Scholar
- Dato V, Weltman A, Waller K, Ruta MA, Highbaugh-Battle A, Hembree C, Evenson S, Wheeler C, Vogt T. 2003. Hepatitis A outbreak associated with green onions at a restaurant—Monaca, Pennsylvania, 2003. MMWR Morb Mortal Wkly Rep 52: 1155–1157.
- Delarocque-Astagneau E, Valenciano M, Dariosecq J-M, Rousselle C, Bouvet E, Laporte A. 2001. An hepatitis A outbreak among MSM in Paris in the year 2000. Bulletin Epidemiologique Hebdomadaire (Paris): 1–3.
- Dentinger CM, Bower WA, Nainan OV, Cotter SM, Myers G, Dubusky LM, Fowler S, Salehi ED, Bell BP. 2001. An outbreak of hepatitis A associated with green onions. J Infect Dis 183: 1273–1276.
- Farfan JA, Olson KE, Black WCt, Gubler DJ, Beaty BJ. 1997. Rapid characterization of genetic diversity among twelve dengue-2 virus isolates by single-strand conformation polymorphism analysis. Am J Trop Med Hyg 57: 416–422.
- Fujiwara K, Yokosuka O, Ehata T, Imazeki F, Saisho H. 2000. PCR-SSCP analysis of 5′-nontranslated region of hepatitis A viral RNA: Comparison with clinicopathological features of hepatitis A. Dig Dis Sci 45: 2422–2427.
- Gigou M, Roque-Afonso AM, Falissard B, Penin F, Dussaix E, Feray C. 2001. Genetic clustering of hepatitis C virus strains and severity of recurrent hepatitis after liver transplantation. J Virol 75: 11292–11297.
- Goswami BB, Burkhardt W III, Cebula TA. 1997. Identification of genetic variants of hepatitis A virus. J Virol Methods 65: 95–103.
-
Hardie DR,
Kannemeyer J,
Stannard LM.
1996.
DNA single strand conformation polymorphism identifies five defined strains of hepatitis B virus (HBV) during an outbreak of HBV infection in an oncology unit.
J Med Virol
49:
49–54.
10.1002/(SICI)1096-9071(199605)49:1<49::AID-JMV8>3.0.CO;2-K CAS PubMed Web of Science® Google Scholar
- Hutin YJ, Pool V, Cramer EH, Nainan OV, Weth J, Williams IT, Goldstein ST, Gensheimer KF, Bell BP, Shapiro CN, Alter MJ, Margolis HS. 1999. A multistate, foodborne outbreak of hepatitis A. National Hepatitis A Investigation Team. N Engl J Med 340: 595–602.
- Koff RS. 1995. Seroepidemiology of hepatitis A in the United States. J Infect Dis 171: S19–S23.
- Kumar S, Tamura K, Jakobsen IB, Nei M. 2001. MEGA2: Molecular evolutionary genetics analysis software. Bioinformatics 17: 1244–1245.
- Maggi F, Fornai C, Vatteroni ML, Giorgi M, Morrica A, Pistello M, Cammarota G, Marchi S, Ciccorossi P, Bionda A, Bendinelli M. 1997. Differences in hepatitis C virus quasispecies composition between liver, peripheral blood mononuclear cells and plasma. J Gen Virol 78: 1521–1525.
- Maisonneuve L, Esteve V, Scart G, Lusina D, Le Pennec MP, Baledent F, Bingen E. 1999. PCR SSCP study of an echovirus 30 meningitis outbreak. Pathol Biol (Paris) 47: 534–538.
- Niu MT, Polish LB, Robertson BH, Khanna BK, Woodruff BA, Shapiro CN, Miller MA, Smith JD, Gedrose JK, Alter MJ, et al. 1992. Multistate outbreak of hepatitis A associated with frozen strawberries. J Infect Dis 166: 518–524.
- Poss M, Rodrigo AG, Gosink JJ, Learn GH, de Vange Panteleeff D, Martin HL, Jr., Bwayo J, Kreiss JK, Overbaugh J. 1998. Evolution of envelope sequences from the genital tract and peripheral blood of women infected with clade A human immunodeficiency virus type 1. J Virol 72: 8240–8251.
- Rezende G, Roque-Afonso AM, Samuel D, Gigou M, Nicand E, Ferre V, Dussaix E, Bismuth H, Feray C. 2003. Viral and clinical factors associated with the fulminant course of hepatitis A infection. Hepatology 38: 613–618.
- Robertson BH, Jansen RW, Khanna B, Totsuka A, Nainan OV, Siegl G, Widell A, Margolis HS, Isomura S, Ito K, et al. 1992. Genetic relatedness of hepatitis A virus strains recovered from different geographical regions. J Gen Virol 73: 1365–1377.
- Robertson BH, Averhoff F, Cromeans TL, Han X, Khoprasert B, Nainan OV, Rosenberg J, Paikoff L, DeBess E, Shapiro CN, Margolis HS. 2000. Genetic relatedness of hepatitis A virus isolates during a community-wide outbreak. J Med Virol 62: 144–150.
- Roque-Afonso AM, Robain M, Simoneau D, Rodriguez-Mathieu P, Gigou M, Meyer L, Dussaix E. 2002. Influence of CD4 cell counts on the genetic heterogeneity of hepatitis C virus in patients coinfected with human immunodeficiency virus. J Infect Dis 185: 728–733.
- Sanchez G, Pinto RM, Vanaclocha H, Bosch A. 2002. Molecular characterization of hepatitis a virus isolates from a transcontinental shellfish-borne outbreak. J Clin Microbiol 40: 4148–4155.
- Sanchez G, Bosch A, Gomez-Mariano G, Domingo E, Pinto RM. 2003. Evidence for quasispecies distributions in the human hepatitis A virus genome. Virology 315: 34–42.
- Stene-Johansen K, Skaug K, Blystad H, Grinde B. 1998. A unique hepatitis A virus strain caused an epidemic in Norway associated with intravenous drug abuse. The Hepatitis A Study Group. Scand J Infect Dis 30: 35–38.
- Stene-Johansen K, Jenum PA, Hoel T, Blystad H, Sunde H, Skaug K. 2002. An outbreak of hepatitis A among homosexuals linked to a family outbreak. Epidemiol Infect 129: 113–117.
- Stroffolini T, Chiaramonte M, Franco E, Rapicetta M, De Mattia D, Mura I, Trivello R, Giammanco A, Rigo G, Scarpa B. 1991. Baseline seroepidemiology of hepatitis A virus infection among children and teenagers in Italy. Infection 19: 97–100.
- Tallo T, Norder H, Tefanova V, Ott K, Ustina V, Prukk T, Solomonova O, Schmidt J, Zilmer K, Priimagi L, Krispin T, Magnius LO. 2003. Sequential changes in hepatitis A virus genotype distribution in Estonia during 1994 to 2001. J Med Virol 70: 187–193.
- Tapia-Conyer R, Santos JI, Cavalcanti AM, Urdaneta E, Rivera L, Manterola A, Potin M, Ruttiman R, Tanaka Kido J. 1999. Hepatitis A in Latin America: A changing epidemiologic pattern. Am J Trop Med Hyg 61: 825–829.
- Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG. 1997. The CLUSTAL_X windows interface: Flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25: 4876–4882.
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