The antigen/antibody systems specific for non-A, non-B hepatitis (NANB) were studied using urine samples as the antigen source and sera for the antibody source. Two immunologically distinct systems, SO-antigen/anti-SO and MI-antigen/anti-MI were discovered. This paper deals chiefly with the characterization of the SO-antigen, which was associated with an epidemic-type non-A, non-B hepatitis found during September 1979 to February 1980 (first outbreak) and October 1980 to January 1981 (second outbreak) in the Cardiovascular Surgical Unit of Tohoku University Hospital. All patients who developed non-A, non-B hepatitis during the first and second epidemic periods had SO-antigen in their urine (24 out of 24). After the epidemic, however, the detection rate of SO-antigen gradually decreased among patients in the same unit, although posttransfusion non-A, non-B hepatitis continued to be found.

The initial detection of SO-antigen occurred at or just after the elevation of alanine aminotransferase (ALT) levels during the episode of hepatitis and persisted in most cases throughout the elevated period. Anti-SO antibody was detected relatively late (eight months after blood transfusion, in most cases) and apparently persisted longer than five years.

The immunological and physicochemical properties of SO-antigen were also studied. It appeared to be neither a plasma protein nor a liver tissue component when the crossreactivity of SO-antigen was examined by the immunodiffusion method. Absorption with insolubilized human serum and liver tissues failed to affect the anti-SO antibody activity. The molecular weight of SO-antigen was estimated to be 250,000, the sedimentation coefficient to be 11.0 S, and the buoyant density in CsCl to be 1.215 g/cm³. Electron microscopy showed that the SO-antigen corresponded with uniform particles with a mean diameter of 11 nm. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) analysis of SO-antigen revealed only a single protein band corresponding to molecular weight 38,000.

References

Almeida JD, Waterson AP (1969): The morphology of virus-antibody interaction. Advances in Virus Research 15: 307–338.
10.1016/S0065-3527(08)60878-7
CAS PubMed Google Scholar
Alter HJ, Purcell RH, Feinstone SM, Tegtmeier GE (1982): Non-A, non-B hepatitis: Its relationship to cytomegalovirus, to chronic hepatitis, and to direct and indirect methods. In W Szmuness, HS Alter, JE Maynard (eds): “ Viral Hepatitis;” 1981 International Symposium. Philadelphia: Franklin Institute Press, pp 279–294.
Google Scholar
Bradley DW, Cook EH, Maynard JE, MacCaustland KA, Ebert JW, Dolana GH, Petzel RA (1979): Experimental infection of chimpanzees with antihemophilic (factor VIII) materials: Recovery of virus-like particles associated with non-A, non-B hepatitis. Journal of Medical Virology 3: 253–269.
10.1002/jmv.1890030403
PubMed Web of Science® Google Scholar
Chircu LV, Pezzella M, Lacava V, Ricci G (1980): Post-transfusion hepatitis: Antigen/antibody systems correlated with non-A, non-B hepatitis. Journal of Medical Virology 6: 147–151.
10.1002/jmv.1890060207
CAS PubMed Web of Science® Google Scholar
Coursaget P, Maupas P, Levin P, Barin F (1979): Virus-like particles associated with non-A, non-B hepatitis. Lancet 2: 92.
10.1016/S0140-6736(79)90137-5
CAS PubMed Web of Science® Google Scholar
Craske J, Spponer RJD, Vandervelde EM (1978): Evidence for the existence of at least two types of factor VIII associated non-A, non-B transfusion hepatitis. Lancet 2: 1051–1052.
10.1016/S0140-6736(78)92374-7
CAS PubMed Web of Science® Google Scholar
Dienstag JL, Alaama A, Mosley JW, Redeker AG, Purcell RH (1977): Etiology of specific hepatitis B surface antigen negative hepatitis. Annals Internal Medicine 87: 1–6.
10.7326/0003-4819-87-1-1
CAS PubMed Web of Science® Google Scholar
Diensteg JL, Bhan AK, Alter HJ, Feinstone SM, Purcell RH (1979): Circulating immune complexes in non-A, non-B hepatitis. Lancet 1: 1265–1267.
10.1016/S0140-6736(79)92228-1
PubMed Web of Science® Google Scholar
Hanshaw JB (1966): Cytomegalovirus complement-fixing antibody microcephaly. New England Journal of Medicine 275: 476–479.
10.1056/NEJM196609012750905
CAS PubMed Web of Science® Google Scholar
Henle G, Henle W (1966): Immunofluorescence in cells derived from Burkitt's lymphoma. Journal of Bacteriology 91: 1248–1256.
PubMed Web of Science® Google Scholar
Henson JB, McGuire TC (1971): Immuno-pathology of equine infectious anemia. American Journal of Clinical Pathology 56: 306–314.
10.1093/ajcp/56.3.306
CAS PubMed Web of Science® Google Scholar
Hirsh MS, Allison AC, Harvey JJ (1969): Immune complexes in mice infected neonatally with Molney leukemogenic and murine sarcoma viruses. Nature 223: 739–740.
10.1038/223739a0
PubMed Web of Science® Google Scholar
Hollinger FB, Gitnick GL, Aach RD, Szmuness W, Mosely JW, Stevens CE, Peters RL, Weiner JM, Wereh JB, Lander JJ (1978): Non-A, non-B hepatitis transmission in chimpanzees: A project of the Transfusion-Transmitted Virus Group. Intervirology 10: 60–68.
10.1159/000148969
PubMed Web of Science® Google Scholar
Hopkins R, Robertson AE, Haase G, Brettle R, Green D, Brown T (1981): Non-A, non-B hepatitis. Lancet 1: 946–947.
10.1016/S0140-6736(81)91643-3
CAS PubMed Web of Science® Google Scholar
Kabiri M, Tabor E, Gerety RJ (1979): Antigen-antibody system associated with non-A, non-B hepatitis detected by indirect immunofluorescence. Lancet 2: 221–224.
10.1016/S0140-6736(79)90237-X
CAS PubMed Web of Science® Google Scholar
Khuroo MS (1980): Study of an epidemic non-A, non-B hepatitis: Possibility of another human hepatitis virus distinct from posttransfusion non-A, non-B type. American Journal of Medicine 68: 818–824.
10.1016/0002-9343(80)90200-4
PubMed Web of Science® Google Scholar
Lehmann H, Meyer GJ, Niedermeyer W, Schlaak M (1982): Frequency of hepatitis B and non-A, non-B hepatitis in a hemodialysis unit from 1975 to 1980. In W Szmuness, HJ Alter, JE Maynard (eds): “ Viral Hepatitis;” 1981 International Symposium. Philadelphia: Franklin Institute Press, pp 774.
Google Scholar
Meyers JD, Dienstag DL, Purcell RH, Thomas ED, Holmes KK (1977): Parenterally transmitted non-A, non-B hepatitis: An epidemic reassessed. Annals of Internal Medicine 87: 57–59.
10.7326/0003-4819-87-1-57
CAS PubMed Web of Science® Google Scholar
Morrison WI, Wright NG (1977): Viruses associated with renal disease of man and animals. Progress in Medical Virology 23: 22–50.
CAS PubMed Google Scholar
Mosley JW, Redeker AG, Feinstone SM, Purcell RH (1977): Multiple hepatitis viruses in multiple attacks of acute viral hepatitis. New England Journal of Medicine 296: 75–78.
10.1056/NEJM197701132960204
PubMed Web of Science® Google Scholar
Neurath AR, Stevens CE, Strick N, Szmuness E, Oleszko R, Harley EJ (1980): An antigen detected frequently in human sera with elevated levels of alanine amino transferase: A potential marker for non-A, non-B hepatitis. The Journal of General Virology 48: 285–295.
10.1099/0022-1317-48-2-285
CAS PubMed Web of Science® Google Scholar
Ohori H, Yamada E, Tateda A, Ishida N (1980): Prevalence of Williams e₁ antigen in comparison with e₂ antigen in hepatitis B antigen carriers and patients in hemodialysis patients. Journal of Medical Virology 6: 61–74.
10.1002/jmv.1890060109
CAS PubMed Web of Science® Google Scholar
Ohori H, Nagatsuka Y, Yamada E, Akiba T, Shirachi R, Shiraishi H, Ishida N (1982): Characterization of immune complexes in sera from patients with non-A, non-B hepatitis. In W Szmuness, HJ Alter, JE Maynard (eds): “ Viral Hepatitis;” 1981 International Symposium. Philadelphia: Franklin Institute Press, pp 331–338.
Google Scholar
Ohori H, Nagatsuka Y, Kanno A, Abe Y, Ishida N (1983): Two distinct types of non-A, non-B hepatitis in a cardiovascular surgical unit. Journal of Medical Virology 11: 105–113.
10.1002/jmv.1890110204
CAS PubMed Web of Science® Google Scholar
Oldstone MBA, Dixon EJ (1969): Pathogenesis of chronic disease associated with persistent lymphocytic chroiomeningitis viral infection. The Journal of Experimental Medicine 129: 483–505.
10.1084/jem.129.3.483
CAS PubMed Web of Science® Google Scholar
Porter DD, Larsen AE (1967): Aleutian disease of mink: Infectious virus-antibody complexes in the serum. Proceedings of the Society for Experimental Biology and Medicine 126: 680–682.
10.3181/00379727-126-32539
Web of Science® Google Scholar
Porter DD, Porter HG (1971): Deposition of immune complexes in the kidneys of mice infected with lactic dehydrogenase virus. Journal of Immunology 106: 1264–1266.
CAS PubMed Web of Science® Google Scholar
Prince AM, Brotman B, Van den Ende MC, Richardson L, Kellner A (1978): Non-A, non-B hepatitis identification of virus-specific antigen and antibody. In GN Vyas, SN Cohen, R Schmid (eds): “ Viral Hepatitis: A Contemporary Assessment of Etiology, Epidemiology, Pathogenesis and Prevention.” Philadelphia: Franklin Institute Press, pp 633–640.
Google Scholar
Robinson WS (1982): The enigma of non-A, non-B hepatitis. The Journal of Infectious Diseases 145: 387–395.
10.1093/infdis/145.3.387
CAS PubMed Web of Science® Google Scholar
Seeff LB, Wright EC, Zimmerman HJ, Hoofnagle JH, Dietz AA, Felsher BF, Garcia-Pont PH, Gerety RH, Greenlee HB, Kierman T, Leevy CM, Nath N, Schiff ER, Schwart C, Tabor E, Tamburro C, Veahcevic Z, Zemel R, Zimmon D (1978): Post-transfusion hepatitis; 1978–1975: A Veterans Administration cooperative study. In GN Vyas, SN Cohen, R Schmid (eds): “ Viral Hepatitis: A Contemporary Assessment of Etiology, Epidemiology, Pathogenesis and Prevention.” Philadelphia: Franklin Institute Press, pp 371–381.
Google Scholar
Shimizu YK, Feinstone SM, Purcell RH, Alter HJ, London WT (1979): Non-A, non-B hepatitis: Ultrastructural evidence for two agents in experimentally infected chimpanzees. Science 205: 197–200.
10.1126/science.451589
CAS PubMed Web of Science® Google Scholar
Shirachi R, Shiraishi H, Tateda A, Kikuchi K, Ishida N (1978): Hepatitis “C” antigen in non-A, non-B posttransfusion hepatitis. Lancet 2: 853–856.
10.1016/S0140-6736(78)91567-2
CAS PubMed Web of Science® Google Scholar
Suh DJ, White Y, Eddleston ALWF, Amini S, Tsiquaye K, Zuckerman AJ, Williams R (1981): Specificity of an immunoprecipitin test for non-A, non-B hepatitis. Lancet 1: 178–180.
10.1016/S0140-6736(81)90059-3
PubMed Web of Science® Google Scholar
Tabor E, Gerety RJ, Druker JA, Seeff LB, Hoofnagle JA, Jackson DR, April M, Barker LF, Pineda-Tamondong G (1978): Transmission of non-A, non-B hepatitis from man to chimpanzee. Lancet 1: 463–466.
10.1016/S0140-6736(78)90132-0
CAS PubMed Web of Science® Google Scholar
Tabor E, Mitchell FD, Goudeau AM, Gerety RJ (1979a): Detection of an antigen-antibody system in serum associated with human non-A, non-B hepatitis. Journal of Medical Virology 4: 161–169.
10.1002/jmv.1890040302
CAS PubMed Web of Science® Google Scholar
Tabor E, April M, Seeff LB, Gerety RJ (1979b): Acquired immunity to human non-A, non-B hepatitis: Cross-challenge of chimpanzees with three infectious human sera. The Journal of Infectious Diseases 140: 789–793.
10.1093/infdis/140.5.789
CAS PubMed Web of Science® Google Scholar
Utz JP (1974): Viruia in man, an update. Progress in Medical Virology 17: 77–90.
CAS PubMed Web of Science® Google Scholar
Villarejos VM, Visona KA, Eduarte CA, Provost PJ, Hilleman MR (1975): Evidence for viral hepatitis other than type A or type B among persons in Costa Rica. New England Journal of Medicine 273: 1350–1352.
10.1056/NEJM197512252932606
Web of Science® Google Scholar
Vitvitski L, Trepo C, Prince AM, Brotman B (1979): Detection of virus-associated antigen in serum and liver of patients with non-A, non-B hepatitis. Lancet 2: 1263–1267.
10.1016/S0140-6736(79)92280-3
PubMed Web of Science® Google Scholar
Yamada E, Ohori H, Ishida N (1981): Molecular heterogeneity of hepatitis B e antigen in liver and serum. Journal of General Virology 55: 75–86.
10.1099/0022-1317-55-1-75
CAS PubMed Web of Science® Google Scholar

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Volume12, Issue3

1983

Pages 161-178

An antigen/antibody system specific for an epidemic non-A, non-B hepatitis in patients of a cardiovascular surgical unit

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An antigen/antibody system specific for an epidemic non-A, non-B hepatitis in patients of a cardiovascular surgical unit

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