Induction of the murine “W phenotype” in long-term cultures of human cord blood cells by c-kit antisense oligomers
Corresponding Author
Anna Rita Migliaccio
Lindsley F. Kimball Research Institute, The New York Blood Center, New York, New York 10021
Lindsley F. Kimball Research Institute, The New York Blood Center, New York, New York 10021Search for more papers by this authorGiovanni Migliaccio
Lindsley F. Kimball Research Institute, The New York Blood Center, New York, New York 10021
Search for more papers by this authorGiancarlo Mancini
Lindsley F. Kimball Research Institute, The New York Blood Center, New York, New York 10021
Search for more papers by this authorMariusz Ratajczak
University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104
Search for more papers by this authorAlan M. Gewirtz
University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104
Search for more papers by this authorJohn W. Adamson
Lindsley F. Kimball Research Institute, The New York Blood Center, New York, New York 10021
Search for more papers by this authorCorresponding Author
Anna Rita Migliaccio
Lindsley F. Kimball Research Institute, The New York Blood Center, New York, New York 10021
Lindsley F. Kimball Research Institute, The New York Blood Center, New York, New York 10021Search for more papers by this authorGiovanni Migliaccio
Lindsley F. Kimball Research Institute, The New York Blood Center, New York, New York 10021
Search for more papers by this authorGiancarlo Mancini
Lindsley F. Kimball Research Institute, The New York Blood Center, New York, New York 10021
Search for more papers by this authorMariusz Ratajczak
University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104
Search for more papers by this authorAlan M. Gewirtz
University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104
Search for more papers by this authorJohn W. Adamson
Lindsley F. Kimball Research Institute, The New York Blood Center, New York, New York 10021
Search for more papers by this authorAbstract
The murine white (W) spotting locus is the site of the c-kit gene and encodes a tyrosine kinase receptor while the complementary Steel (Sl) iocus encodes its ligand. Mutations at either locus have profound effects on hematopoiesis, particularly erythroid and mast cell proliferation. We added c-kit antisense oligonucleotides to long-term suspension cultures of enriched human umbilical cord progenitor cells. This resulted in the suppression of c-kit gene expression and the preferential suppression of the generation of erythroid burst-forming cells (BFU-E) which extended over the life of the culture (3 weeks). The results provide an in vitro model of the “W phenotype” in human hematopoiesis and confirm the importance of c-kit gene function in early erythropoiesis. Because the generation of BFU-E was suppressed even after c-kit gene expression had recovered, this gene product may be critical to the erythroid commitment process. © 1993 Wiley-Liss, Inc.
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