Decellularised Amniotic Membrane for the Neurogenic Expression of Human Mesenchymal Stem Cells
Jingwen Wu
Technical Institute of Physics and Chemistry, University of Chinese Academy of Sciences, Beijing, China
Search for more papers by this authorYantong Wang
Laboratory of Molecular Signaling and Stem Cells Therapy, Beijing Key Laboratory of Tooth Regeneration and Function Reconstruction, Capital Medical University School of Stomatology, Beijing, China
Search for more papers by this authorTong Zhang
Technical Institute of Physics and Chemistry, University of Chinese Academy of Sciences, Beijing, China
Search for more papers by this authorFenglin Yu
Technical Institute of Physics and Chemistry, University of Chinese Academy of Sciences, Beijing, China
Search for more papers by this authorYunci Wang
Technical Institute of Physics and Chemistry, University of Chinese Academy of Sciences, Beijing, China
Search for more papers by this authorXiaoyong Ran
Weihai Yinhe Biological Technology Co., Ltd, Weihai, Huancui District, China
Search for more papers by this authorQi Hao
Weihai Yinhe Biological Technology Co., Ltd, Weihai, Huancui District, China
Search for more papers by this authorCorresponding Author
Yangyang Cao
Laboratory of Molecular Signaling and Stem Cells Therapy, Beijing Key Laboratory of Tooth Regeneration and Function Reconstruction, Capital Medical University School of Stomatology, Beijing, China
Correspondence:
Yangyang Cao ([email protected])
Yanchuan Guo ([email protected])
Search for more papers by this authorCorresponding Author
Yanchuan Guo
Technical Institute of Physics and Chemistry, University of Chinese Academy of Sciences, Beijing, China
Correspondence:
Yangyang Cao ([email protected])
Yanchuan Guo ([email protected])
Search for more papers by this authorJingwen Wu
Technical Institute of Physics and Chemistry, University of Chinese Academy of Sciences, Beijing, China
Search for more papers by this authorYantong Wang
Laboratory of Molecular Signaling and Stem Cells Therapy, Beijing Key Laboratory of Tooth Regeneration and Function Reconstruction, Capital Medical University School of Stomatology, Beijing, China
Search for more papers by this authorTong Zhang
Technical Institute of Physics and Chemistry, University of Chinese Academy of Sciences, Beijing, China
Search for more papers by this authorFenglin Yu
Technical Institute of Physics and Chemistry, University of Chinese Academy of Sciences, Beijing, China
Search for more papers by this authorYunci Wang
Technical Institute of Physics and Chemistry, University of Chinese Academy of Sciences, Beijing, China
Search for more papers by this authorXiaoyong Ran
Weihai Yinhe Biological Technology Co., Ltd, Weihai, Huancui District, China
Search for more papers by this authorQi Hao
Weihai Yinhe Biological Technology Co., Ltd, Weihai, Huancui District, China
Search for more papers by this authorCorresponding Author
Yangyang Cao
Laboratory of Molecular Signaling and Stem Cells Therapy, Beijing Key Laboratory of Tooth Regeneration and Function Reconstruction, Capital Medical University School of Stomatology, Beijing, China
Correspondence:
Yangyang Cao ([email protected])
Yanchuan Guo ([email protected])
Search for more papers by this authorCorresponding Author
Yanchuan Guo
Technical Institute of Physics and Chemistry, University of Chinese Academy of Sciences, Beijing, China
Correspondence:
Yangyang Cao ([email protected])
Yanchuan Guo ([email protected])
Search for more papers by this authorFunding: This work was supported by the National Key Research &Development Program of China (2022YFE0106000), the Postdoctoral Merit Grants in Zhejiang Province (ZJ2024159), and the National Natural Science Foundation of China (82301019, 82401173).
Jingwen Wu and Yantong Wang contributed equally and share the first authorship.
ABSTRACT
To observe the induction of neurogenic differentiation in human mesenchymal stem cells (hMSCs) by decellularized amniotic membrane (DAM), thereby promoting neural regeneration for peripheral neuropathy. Subcutaneous implantation and immunofluorescence staining were conducted to observe the condition of neural cells. Cell adhesion and viability were evaluated through adhesion assays and live/dead cell staining on the DAM. Spatial transcriptomics sequencing was performed to analyze the expression of genes related to adhesion and neural differentiation. Subsequently, stem cells were seeded onto the DAM, and immunofluorescence staining was used to observe neural cell markers and cell migration capabilities. Finally, a network pharmacological analysis, based on the spatial transcriptome results, was performed to identify neurological-related disorders that may be treated by DAM. The cell adhesion assays showed an increased number of adherent cells with normal morphology. Spatial transcriptomics analysis indicated that the DAM significantly upregulated genes associated with cell adhesion and neural differentiation. Immunofluorescence staining revealed that the DAM significantly induced the expression of neural marker proteins. Lastly, subcutaneous implantation demonstrated the aggregation of neural-related cells. DAM can promote stem cell adhesion, induce cell migration, and thereby enhance neural repair and regeneration in cases of peripheral neuropathy.
Conflicts of Interest
The authors declare no conflicts of interest.
Open Research
Data Availability Statement
The data that support the findings of this study are available from the corresponding author upon reasonable request.
Supporting Information
Filename | Description |
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jbmb35588-sup-0001-Figures.docxWord 2007 document , 1.6 MB |
Figure S1. Cellular Adhesion and Morphology on DAM and NAM Figure S2. Transcriptome Differential Genes between NAM and DAM (GO analysis) Figure S3. Transcriptome Differential Genes between NAM and DAM (KEGG analysis) Figure S4. Cellular Neuronal Differentiation on NAM and DAM Figure S5. Cell Migration on NAM and DAM Figure S6. The network pharmacology analysis of peripheral neuropathy between NAM and DAM. |
Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.
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