Volume 113, Issue 6 e35588
RESEARCH ARTICLE

Decellularised Amniotic Membrane for the Neurogenic Expression of Human Mesenchymal Stem Cells

Jingwen Wu

Jingwen Wu

Technical Institute of Physics and Chemistry, University of Chinese Academy of Sciences, Beijing, China

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Yantong Wang

Yantong Wang

Laboratory of Molecular Signaling and Stem Cells Therapy, Beijing Key Laboratory of Tooth Regeneration and Function Reconstruction, Capital Medical University School of Stomatology, Beijing, China

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Tong Zhang

Tong Zhang

Technical Institute of Physics and Chemistry, University of Chinese Academy of Sciences, Beijing, China

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Fenglin Yu

Fenglin Yu

Technical Institute of Physics and Chemistry, University of Chinese Academy of Sciences, Beijing, China

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Yunci Wang

Yunci Wang

Technical Institute of Physics and Chemistry, University of Chinese Academy of Sciences, Beijing, China

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Xiaoyong Ran

Xiaoyong Ran

Weihai Yinhe Biological Technology Co., Ltd, Weihai, Huancui District, China

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Qi Hao

Qi Hao

Weihai Yinhe Biological Technology Co., Ltd, Weihai, Huancui District, China

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Yangyang Cao

Corresponding Author

Yangyang Cao

Laboratory of Molecular Signaling and Stem Cells Therapy, Beijing Key Laboratory of Tooth Regeneration and Function Reconstruction, Capital Medical University School of Stomatology, Beijing, China

Correspondence:

Yangyang Cao ([email protected])

Yanchuan Guo ([email protected])

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Yanchuan Guo

Corresponding Author

Yanchuan Guo

Technical Institute of Physics and Chemistry, University of Chinese Academy of Sciences, Beijing, China

Correspondence:

Yangyang Cao ([email protected])

Yanchuan Guo ([email protected])

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First published: 27 May 2025

Funding: This work was supported by the National Key Research &Development Program of China (2022YFE0106000), the Postdoctoral Merit Grants in Zhejiang Province (ZJ2024159), and the National Natural Science Foundation of China (82301019, 82401173).

Jingwen Wu and Yantong Wang contributed equally and share the first authorship.

ABSTRACT

To observe the induction of neurogenic differentiation in human mesenchymal stem cells (hMSCs) by decellularized amniotic membrane (DAM), thereby promoting neural regeneration for peripheral neuropathy. Subcutaneous implantation and immunofluorescence staining were conducted to observe the condition of neural cells. Cell adhesion and viability were evaluated through adhesion assays and live/dead cell staining on the DAM. Spatial transcriptomics sequencing was performed to analyze the expression of genes related to adhesion and neural differentiation. Subsequently, stem cells were seeded onto the DAM, and immunofluorescence staining was used to observe neural cell markers and cell migration capabilities. Finally, a network pharmacological analysis, based on the spatial transcriptome results, was performed to identify neurological-related disorders that may be treated by DAM. The cell adhesion assays showed an increased number of adherent cells with normal morphology. Spatial transcriptomics analysis indicated that the DAM significantly upregulated genes associated with cell adhesion and neural differentiation. Immunofluorescence staining revealed that the DAM significantly induced the expression of neural marker proteins. Lastly, subcutaneous implantation demonstrated the aggregation of neural-related cells. DAM can promote stem cell adhesion, induce cell migration, and thereby enhance neural repair and regeneration in cases of peripheral neuropathy.

Conflicts of Interest

The authors declare no conflicts of interest.

Data Availability Statement

The data that support the findings of this study are available from the corresponding author upon reasonable request.

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