Development of surface functionalization strategies for 3D-printed polystyrene constructs
Max J. Lerman
Department of Materials Science and Engineering, University of Maryland, College Park, Maryland
Surface and Trace Chemical Analysis Group, Materials Measurement Laboratory, National Institute of Standards and Technology, Gaithersburg, Maryland
Center for Engineering Complex Tissues, University of Maryland, College Park, Maryland
Search for more papers by this authorShin Muramoto
Surface and Trace Chemical Analysis Group, Materials Measurement Laboratory, National Institute of Standards and Technology, Gaithersburg, Maryland
Search for more papers by this authorNavein Arumugasaamy
Center for Engineering Complex Tissues, University of Maryland, College Park, Maryland
Fischell Department of Bioengineerin, University of Maryland, College Park, Maryland
Sheikh Zayed Institute for Pediatric Surgical Innovation, Children's National Health System, Washington, District of Columbia
Search for more papers by this authorMichael Van Order
Department of Materials Science and Engineering, University of Maryland, College Park, Maryland
Search for more papers by this authorJosephine Lembong
Center for Engineering Complex Tissues, University of Maryland, College Park, Maryland
Fischell Department of Bioengineerin, University of Maryland, College Park, Maryland
Search for more papers by this authorAnushka G. Gerald
Center for Engineering Complex Tissues, University of Maryland, College Park, Maryland
Fischell Department of Bioengineerin, University of Maryland, College Park, Maryland
Search for more papers by this authorGreg Gillen
Surface and Trace Chemical Analysis Group, Materials Measurement Laboratory, National Institute of Standards and Technology, Gaithersburg, Maryland
Search for more papers by this authorCorresponding Author
John P. Fisher
Center for Engineering Complex Tissues, University of Maryland, College Park, Maryland
Fischell Department of Bioengineerin, University of Maryland, College Park, Maryland
Correspondence to: J. P. Fisher; e-mail: [email protected]Search for more papers by this authorMax J. Lerman
Department of Materials Science and Engineering, University of Maryland, College Park, Maryland
Surface and Trace Chemical Analysis Group, Materials Measurement Laboratory, National Institute of Standards and Technology, Gaithersburg, Maryland
Center for Engineering Complex Tissues, University of Maryland, College Park, Maryland
Search for more papers by this authorShin Muramoto
Surface and Trace Chemical Analysis Group, Materials Measurement Laboratory, National Institute of Standards and Technology, Gaithersburg, Maryland
Search for more papers by this authorNavein Arumugasaamy
Center for Engineering Complex Tissues, University of Maryland, College Park, Maryland
Fischell Department of Bioengineerin, University of Maryland, College Park, Maryland
Sheikh Zayed Institute for Pediatric Surgical Innovation, Children's National Health System, Washington, District of Columbia
Search for more papers by this authorMichael Van Order
Department of Materials Science and Engineering, University of Maryland, College Park, Maryland
Search for more papers by this authorJosephine Lembong
Center for Engineering Complex Tissues, University of Maryland, College Park, Maryland
Fischell Department of Bioengineerin, University of Maryland, College Park, Maryland
Search for more papers by this authorAnushka G. Gerald
Center for Engineering Complex Tissues, University of Maryland, College Park, Maryland
Fischell Department of Bioengineerin, University of Maryland, College Park, Maryland
Search for more papers by this authorGreg Gillen
Surface and Trace Chemical Analysis Group, Materials Measurement Laboratory, National Institute of Standards and Technology, Gaithersburg, Maryland
Search for more papers by this authorCorresponding Author
John P. Fisher
Center for Engineering Complex Tissues, University of Maryland, College Park, Maryland
Fischell Department of Bioengineerin, University of Maryland, College Park, Maryland
Correspondence to: J. P. Fisher; e-mail: [email protected]Search for more papers by this authorAbstract
There is a growing interest in 3D printing to fabricate culture substrates; however, the surface properties of the scaffold remain pertinent to elicit targeted and expected cell responses. Traditional 2D polystyrene (PS) culture systems typically require surface functionalization (oxidation) to facilitate and encourage cell adhesion. Determining the surface properties which enhance protein adhesion from media and cellular extracellular matrix (ECM) production remains the first step to translating 2D PS systems to a 3D culture surface. Here we show that the presence of carbonyl groups to PS surfaces correlated well with successful adhesion of ECM proteins and sustaining ECM production of deposited human mesenchymal stem cells, if the surface has a water contact angle between 50° and 55°. Translation of these findings to custom-fabricated 3D PS scaffolds reveals carbonyl groups continued to enhance spreading and growth in 3D culture. Cumulatively, these data present a method for 3D printing PS and the design considerations required for understanding cell–material interactions. © 2019 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 107B:2566–2578, 2019.
CONFLICT OF INTEREST
JPF is a founding member of, and has a financial interest in, 3DBioWorks, which focuses on developing bioreactor technology.
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