Scaffolds for epithelial tissue engineering customized in elastomeric molds
Mohamed-Nur Abdallah
Faculty of Dentistry, McGill University, Montreal, Quebec, Canada
Search for more papers by this authorSara Abdollahi
Department of Mining and Materials Engineering, McGill University, Montreal, Quebec, Canada
Search for more papers by this authorMarco Laurenti
Faculty of Dentistry, McGill University, Montreal, Quebec, Canada
Search for more papers by this authorDongdong Fang
Faculty of Dentistry, McGill University, Montreal, Quebec, Canada
Craniofacial Stem Cells and Tissue Engineering Laboratory, McGill University, Montreal, Quebec, Canada
Search for more papers by this authorSimon D Tran
Faculty of Dentistry, McGill University, Montreal, Quebec, Canada
Craniofacial Stem Cells and Tissue Engineering Laboratory, McGill University, Montreal, Quebec, Canada
Search for more papers by this authorMarta Cerruti
Department of Mining and Materials Engineering, McGill University, Montreal, Quebec, Canada
Search for more papers by this authorCorresponding Author
Faleh Tamimi
Faculty of Dentistry, McGill University, Montreal, Quebec, Canada
Correspondence to: F. Tamimi; e-mail: [email protected]Search for more papers by this authorMohamed-Nur Abdallah
Faculty of Dentistry, McGill University, Montreal, Quebec, Canada
Search for more papers by this authorSara Abdollahi
Department of Mining and Materials Engineering, McGill University, Montreal, Quebec, Canada
Search for more papers by this authorMarco Laurenti
Faculty of Dentistry, McGill University, Montreal, Quebec, Canada
Search for more papers by this authorDongdong Fang
Faculty of Dentistry, McGill University, Montreal, Quebec, Canada
Craniofacial Stem Cells and Tissue Engineering Laboratory, McGill University, Montreal, Quebec, Canada
Search for more papers by this authorSimon D Tran
Faculty of Dentistry, McGill University, Montreal, Quebec, Canada
Craniofacial Stem Cells and Tissue Engineering Laboratory, McGill University, Montreal, Quebec, Canada
Search for more papers by this authorMarta Cerruti
Department of Mining and Materials Engineering, McGill University, Montreal, Quebec, Canada
Search for more papers by this authorCorresponding Author
Faleh Tamimi
Faculty of Dentistry, McGill University, Montreal, Quebec, Canada
Correspondence to: F. Tamimi; e-mail: [email protected]Search for more papers by this authorAbstract
Restoration of soft tissue defects remains a challenge for surgical reconstruction. In this study, we introduce a new approach to fabricate poly(d,l-lactic acid) (PDLLA) scaffolds with anatomical shapes customized to regenerate three-dimensional soft tissue defects. Highly concentrated polymer/salt mixtures were molded in flexible polyether molds. Microcomputed tomography showed that with this approach it was possible to produce scaffolds with clinically acceptable volume ratio maintenance (>90%). Moreover, this technique allowed us to customize the average pore size and pore interconnectivity of the scaffolds by using variations of salt particle size. In addition, this study demonstrated that with the increasing porosity and/or the decreasing of the average pore size of the PDLLA scaffolds, their mechanical properties decrease and they degrade more slowly. Cell culture results showed that PDLLA scaffolds with an average pore size of 100 µm enhance the viability and proliferation rates of human gingival epithelial cells up to 21 days. The simple method proposed in this article can be extended to fabricate porous scaffolds with customizable anatomical shapes and optimal pore structure for epithelial tissue engineering. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 106B: 880–890, 2018.
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