Lithium doped calcium phosphate cement maintains physical mechanical properties and promotes osteoblast proliferation and differentiation
Li Li
The Fourth Affiliated Hospital of Guangxi Medical University/Liu Zhou Work's Hospital, Liuzhou, Guangxi, 545005 China
Both authors contributed equally to this work.
Search for more papers by this authorRenchong Wang
The Fourth Affiliated Hospital of Guangxi Medical University/Liu Zhou Work's Hospital, Liuzhou, Guangxi, 545005 China
Both authors contributed equally to this work.
Search for more papers by this authorBaichuan Li
The Fourth Affiliated Hospital of Guangxi Medical University/Liu Zhou Work's Hospital, Liuzhou, Guangxi, 545005 China
Search for more papers by this authorWei Liang
The Fourth Affiliated Hospital of Guangxi Medical University/Liu Zhou Work's Hospital, Liuzhou, Guangxi, 545005 China
Search for more papers by this authorHaobo Pan
Center for Human Tissues and Organs Degeneration, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen, Guangdong, 518055 China
Search for more papers by this authorXu Cui
Center for Human Tissues and Organs Degeneration, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen, Guangdong, 518055 China
Search for more papers by this authorJingli Tang
The Fourth Affiliated Hospital of Guangxi Medical University/Liu Zhou Work's Hospital, Liuzhou, Guangxi, 545005 China
Search for more papers by this authorCorresponding Author
Bing Li
The Fourth Affiliated Hospital of Guangxi Medical University/Liu Zhou Work's Hospital, Liuzhou, Guangxi, 545005 China
Correspondence to: B. Li; e-mail: [email protected]Search for more papers by this authorLi Li
The Fourth Affiliated Hospital of Guangxi Medical University/Liu Zhou Work's Hospital, Liuzhou, Guangxi, 545005 China
Both authors contributed equally to this work.
Search for more papers by this authorRenchong Wang
The Fourth Affiliated Hospital of Guangxi Medical University/Liu Zhou Work's Hospital, Liuzhou, Guangxi, 545005 China
Both authors contributed equally to this work.
Search for more papers by this authorBaichuan Li
The Fourth Affiliated Hospital of Guangxi Medical University/Liu Zhou Work's Hospital, Liuzhou, Guangxi, 545005 China
Search for more papers by this authorWei Liang
The Fourth Affiliated Hospital of Guangxi Medical University/Liu Zhou Work's Hospital, Liuzhou, Guangxi, 545005 China
Search for more papers by this authorHaobo Pan
Center for Human Tissues and Organs Degeneration, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen, Guangdong, 518055 China
Search for more papers by this authorXu Cui
Center for Human Tissues and Organs Degeneration, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen, Guangdong, 518055 China
Search for more papers by this authorJingli Tang
The Fourth Affiliated Hospital of Guangxi Medical University/Liu Zhou Work's Hospital, Liuzhou, Guangxi, 545005 China
Search for more papers by this authorCorresponding Author
Bing Li
The Fourth Affiliated Hospital of Guangxi Medical University/Liu Zhou Work's Hospital, Liuzhou, Guangxi, 545005 China
Correspondence to: B. Li; e-mail: [email protected]Search for more papers by this authorDisclosure: The authors declared no competing interest.
Abstract
Calcium phosphate cement (CPC) has been widely used in bone tissue repairing due to its physical mechanical properties and biocompatibility. Addition of trace element to CPC has shown promising evidence to improve the physical properties and biological activities of CPC. Lithium (Li) has effect on osteoblast proliferation and differentiation. In this study, we incorporated Li to CPC and examined the physical properties of Li/CPC and its effect on osteoblast proliferation and differentiation. We found that Li doped CPC maintained similar setting time, pore size distribution, compressive strength, composition, and morphology as CPC without Li. Additionally, Li doped CPC improved osteoblast proliferation and differentiation significantly compared to CPC without Li. To our knowledge, our results, for the first time, show that Li doped CPC has beneficial effect on osteoblast in cell culture while keeps the excellent physical–mechanical properties of CPC. This study will lead to potential application of Li doped CPC in bone tissue engineering. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 105B: 944–952, 2017.
REFERENCES
- 1 Ambard AJ, Mueninghoff L. Calcium phosphate cement: Review of mechanical and biological properties. J Prosthodont 2006; 15: 321–328.
- 2 Cade JFJ. Next generation calcium phosphate-based biomaterials. Dent Mater J 2009; 28: 1–10.
- 3 Cherng AM, Chow LC, Takagi S. Calcium orthophosphate cements for biomedical application. J Mater Sci 2008; 43: 3028–3057.
- 4 Chow LC. Bone tissue engineering via nanostructured calcium phosphate biomaterials and stem cells. Bone Res 2014; 2: 14017.
- 5 Davis LA, Zur Nieden NI. Nanomaterials: The next step in injectable bone cements. Nanomedicine (Lond) 2014; 9: 1745–1764.
- 6 Dorozhkin SV. Calcium phosphate ceramics in bone tissue engineering: A review of properties and their influence on cell behavior. Acta Biomater 2013; 9: 8037–8045.
- 7 Eldar-Finkelman H, Martinez A. Injectable calcium phosphate cement for bone repair and implant fixation. Orthop Clin North Am 2015; 36: 89–95, vii.
- 8 Ginebra MP, Canal C, Espanol M, Pastorino D, Montufar EB. Calcium phosphate-based composites as injectable bone substitute materials. J Biomed Mater Res B: Appl Biomater 94B: 273–286.
- 9 Ginebra MP, Fernandez E, Boltong MG, Bermudez O, Planell JA, Driessens FC. In vitro evaluation of a calcium phosphate cement root canal filler/sealer. J Endod 2001; 27: 613–615.
- 10 Glass DA, 2nd, Karsenty G. Osteoblastic induction on calcium phosphate cement-chitosan constructs for bone tissue engineering. J Biomed Mater Res A 2010; 94: 223–233.
- 11 Han P, Wu C, Chang J, Xiao Y. A novel injectable calcium phosphate cement-bioactive glass composite for bone regeneration. PLoS One 2013; 8: e62570.
- 12 He F, Chen Y, Li J, Lin B, Ouyang Y, Yu B, Xia Y, Ye J. Calcium phosphate cements as drug delivery materials. Adv Drug Deliv Rev 2012; 64: 1090–1110.
- 13 Jansen J, Ooms E, Verdonschot N, Wolke J. Microstructure and properties of alendronate-loaded calcium phosphate cement. Mater Sci Eng C Mater Biol Appl 2014; 42: 303–311.
- 14 Kuang GM, Yau WP, Lam WM, Wu J, Chiu KY, Lu WW, Pan H. Improving bone repair of femoral and radial defects in rabbit by incorporating PRP into PLGA/CPC composite scaffold with unidirectional pore structure. J Biomed Mater Res A 2015; 103: 1312–1324.
- 15 Kuang GM, Yau WP, Wu J, Yeung KW, Pan H, Lam WM, Lu WW, Chiu KY. An effective approach by a chelate reaction in optimizing the setting process of strontium-incorporated calcium phosphate bone cement. J Biomed Mater Res B Appl Biomater 2012; 100: 778–787.
- 16 Low KL, Tan SH, S Zein SH, Roether JA, Mourino V, Boccaccini AR. Strontium exerts dual effects on calcium phosphate cement: Accelerating the degradation and enhancing the osteoconductivity both in vitro and in vivo. J Biomed Mater Res A 2015; 103: 1613–1621.
- 17 Makoukji J, Belle M, Meffre D, Stassart R, Grenier J, Shackleford G, Fledrich R, Fonte C, Branchu J, Goulard M, de Waele C, Charbonnier F, Sereda MW, Baulieu EE, Schumacher M, Bernard S, Massaad C. Self-setting bioactive calcium-magnesium phosphate cement with high strength and degradability for bone regeneration. Acta Biomater 2008; 4: 1873–1884.
- 18 No YJ, Roohani-Esfahani SI, Zreiqat H. Lithium chloride regulates the proliferation of stem-like cells in retinoblastoma cell lines: a potential role for the canonical Wnt signaling pathway. Mol Vis 2010; 16: 36–45.
- 19 Samavedi S, Whittington AR, Goldstein AS. Molecular bases of the regulation of bone remodeling by the canonical Wnt signaling pathway. Curr Top Dev Biol 2006; 73: 43–84.
- 20 Shen Z, Yu T, Ye J. Mesodermal fate decisions of a stem cell: The Wnt switch. Cell Mol Life Sci 2008; 65: 2658–2674.
- 21 Silva AK, Yi H, Hayes SH, Seigel GM, Hackam AS. Microstructure and biomechanical characteristics of bone substitutes for trauma and orthopaedic surgery. BMC Musculoskelet Disord 2011; 12: 34.
- 22 Takahashi-Yanaga F. Compliance of an apatitic calcium phosphate cement with the short-term clinical requirements in bone surgery, orthopaedics and dentistry. Clin Mater 1994; 17: 99–104.
- 23 Van Lieshout EM, Van Kralingen GH, El-Massoudi Y, Weinans H, Patka P. GSK-3 Inhibitors: Preclinical and Clinical Focus on CNS. Front Mol Neurosci 2011; 4: 32.
- 24 Wang P, Zhao L, Liu J, Weir MD, Zhou X, Xu HH. Activator or inhibitor? GSK-3 as a new drug target. Biochem Pharmacol 2013; 86: 191–199.
- 25 Weir MD, Xu HH. The cementogenic differentiation of periodontal ligament cells via the activation of Wnt/beta-catenin signalling pathway by Li+ ions released from bioactive scaffolds. Biomaterials 2012; 33: 6370–6379.
- 26 Wu F, Wei J, Guo H, Chen F, Hong H, Liu C. Lithium enhances remyelination of peripheral nerves. Proc Natl Acad Sci USA 2012; 109: 3973–3978.
- 27 Yu L, Li Y, Zhao K, Tang Y, Cheng Z, Chen J, Zang Y, Wu J, Kong L, Liu S, Lei W, Wu Z. Lithium salts in the treatment of psychotic excitement. Med J Aust 1949; 2: 349–352.
