Polycaprolactone–thiophene-conjugated carbon nanotube meshes as scaffolds for cardiac progenitor cells
Abeni M. Wickham
Integrative Regenerative Medicine (IGEN) Centre and Department of Physics, Chemistry and Biology, Linköping University, Linköping, Sweden
*These authors contributed equally to this work.
Search for more papers by this authorM. Mirazul Islam
Swedish Medical Nanoscience Centre Department of Neuroscience, Karolinska Institutet, Stockholm, Sweden
Integrative Regenerative Medicine (IGEN) Centre and Department of Clinical and Experimental Medicine, Linköping University, Linköping, Sweden
*These authors contributed equally to this work.
Search for more papers by this authorDebasish Mondal
Integrative Regenerative Medicine (IGEN) Centre and Department of Physics, Chemistry and Biology, Linköping University, Linköping, Sweden
*These authors contributed equally to this work.
Search for more papers by this authorJaywant Phopase
Integrative Regenerative Medicine (IGEN) Centre and Department of Physics, Chemistry and Biology, Linköping University, Linköping, Sweden
Search for more papers by this authorVeera Sadhu
Integrative Regenerative Medicine (IGEN) Centre and Department of Physics, Chemistry and Biology, Linköping University, Linköping, Sweden
Search for more papers by this authorÉva Tamás
Division of Cardiovascular Diseases Department of Medicine and Health Sciences, Linköping University, Sweden
Department of Cardiothoracic Surgery UHL, County Council of Östergötland, Linköping, Sweden
Search for more papers by this authorNaresh Polisetti
Integrative Regenerative Medicine (IGEN) Centre and Department of Clinical and Experimental Medicine, Linköping University, Linköping, Sweden
Search for more papers by this authorAgneta Richter-Dahlfors
Swedish Medical Nanoscience Centre Department of Neuroscience, Karolinska Institutet, Stockholm, Sweden
Search for more papers by this authorBo Liedberg
Integrative Regenerative Medicine (IGEN) Centre and Department of Physics, Chemistry and Biology, Linköping University, Linköping, Sweden
Centre for Biomimetic Sensor Science, Nanyang Technological University, Singapore
Search for more papers by this authorCorresponding Author
May Griffith
Swedish Medical Nanoscience Centre Department of Neuroscience, Karolinska Institutet, Stockholm, Sweden
Integrative Regenerative Medicine (IGEN) Centre and Department of Clinical and Experimental Medicine, Linköping University, Linköping, Sweden
Correspondence to: M. Griffith (e-mail: [email protected])Search for more papers by this authorAbeni M. Wickham
Integrative Regenerative Medicine (IGEN) Centre and Department of Physics, Chemistry and Biology, Linköping University, Linköping, Sweden
*These authors contributed equally to this work.
Search for more papers by this authorM. Mirazul Islam
Swedish Medical Nanoscience Centre Department of Neuroscience, Karolinska Institutet, Stockholm, Sweden
Integrative Regenerative Medicine (IGEN) Centre and Department of Clinical and Experimental Medicine, Linköping University, Linköping, Sweden
*These authors contributed equally to this work.
Search for more papers by this authorDebasish Mondal
Integrative Regenerative Medicine (IGEN) Centre and Department of Physics, Chemistry and Biology, Linköping University, Linköping, Sweden
*These authors contributed equally to this work.
Search for more papers by this authorJaywant Phopase
Integrative Regenerative Medicine (IGEN) Centre and Department of Physics, Chemistry and Biology, Linköping University, Linköping, Sweden
Search for more papers by this authorVeera Sadhu
Integrative Regenerative Medicine (IGEN) Centre and Department of Physics, Chemistry and Biology, Linköping University, Linköping, Sweden
Search for more papers by this authorÉva Tamás
Division of Cardiovascular Diseases Department of Medicine and Health Sciences, Linköping University, Sweden
Department of Cardiothoracic Surgery UHL, County Council of Östergötland, Linköping, Sweden
Search for more papers by this authorNaresh Polisetti
Integrative Regenerative Medicine (IGEN) Centre and Department of Clinical and Experimental Medicine, Linköping University, Linköping, Sweden
Search for more papers by this authorAgneta Richter-Dahlfors
Swedish Medical Nanoscience Centre Department of Neuroscience, Karolinska Institutet, Stockholm, Sweden
Search for more papers by this authorBo Liedberg
Integrative Regenerative Medicine (IGEN) Centre and Department of Physics, Chemistry and Biology, Linköping University, Linköping, Sweden
Centre for Biomimetic Sensor Science, Nanyang Technological University, Singapore
Search for more papers by this authorCorresponding Author
May Griffith
Swedish Medical Nanoscience Centre Department of Neuroscience, Karolinska Institutet, Stockholm, Sweden
Integrative Regenerative Medicine (IGEN) Centre and Department of Clinical and Experimental Medicine, Linköping University, Linköping, Sweden
Correspondence to: M. Griffith (e-mail: [email protected])Search for more papers by this authorAbstract
The myocardium is unable to regenerate itself after infarct, resulting in scarring and thinning of the heart wall. Our objective was to develop a patch to buttress and bypass the scarred area, while allowing regeneration by incorporated cardiac stem/progenitor cells (CPCs). Polycaprolactone (PCL) was fabricated as both sheets by solvent casting, and fibrous meshes by electrospinning, as potential patches, to determine the role of topology in proliferation and phenotypic changes to the CPCs. Thiophene-conjugated carbon nanotubes (T-CNTs) were incorporated to enhance the mechanical strength. We showed that freshly isolated CPCs from murine hearts neither attached nor spread on the PCL sheets, both with and without T-CNT. As electrospun meshes, however, both PCL and PCL/T-CNT supported CPC adhesion, proliferation, and differentiation. The incorporation of T-CNT into PCL resulted in a significant increase in mechanical strength but no morphological changes to the meshes. In turn, proliferation, but not differentiation, of CPCs into cardiomyocytes was enhanced in T-CNT containing meshes. We have shown that changing the topology of PCL, a known hydrophobic material, dramatically altered its properties, in this case, allowing CPCs to survive and differentiate. With further development, PCL/T-CNT meshes or similar patches may become a viable strategy to aid restoration of the postmyocardial infarction myocardium. © 2014 Wiley Periodicals, Inc. J Biomed Mater Res Part B, 102B: 1553–1561, 2014.
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