Biocompatibility of submicron Bioglass® powders obtained by a top-down approach
Anja Dörfler
Friedrich-Alexander-University Erlangen-Nuremberg, Institute of Biomaterials (WW 7), Cauerstraße 6, 91058 Erlangen, Germany
These authors contributed equally and share the first authorship for this article.
Search for more papers by this authorRainer Detsch
Friedrich-Alexander-University Erlangen-Nuremberg, Institute of Biomaterials (WW 7), Cauerstraße 6, 91058 Erlangen, Germany
These authors contributed equally and share the first authorship for this article.
Search for more papers by this authorStefan Romeis
Friedrich-Alexander-University Erlangen-Nuremberg, Institute of Particle Technology (LFG), Cauerstraße 4, 91058 Erlangen, Germany
These authors contributed equally and share the first authorship for this article.
Search for more papers by this authorJochen Schmidt
Friedrich-Alexander-University Erlangen-Nuremberg, Institute of Particle Technology (LFG), Cauerstraße 4, 91058 Erlangen, Germany
Search for more papers by this authorClaudia Eisermann
Friedrich-Alexander-University Erlangen-Nuremberg, Institute of Particle Technology (LFG), Cauerstraße 4, 91058 Erlangen, Germany
Search for more papers by this authorWolfgang Peukert
Friedrich-Alexander-University Erlangen-Nuremberg, Institute of Particle Technology (LFG), Cauerstraße 4, 91058 Erlangen, Germany
Search for more papers by this authorCorresponding Author
Aldo R. Boccaccini
Friedrich-Alexander-University Erlangen-Nuremberg, Institute of Biomaterials (WW 7), Cauerstraße 6, 91058 Erlangen, Germany
Correspondence to: A. R. Boccaccini (e-mail: [email protected])Search for more papers by this authorAnja Dörfler
Friedrich-Alexander-University Erlangen-Nuremberg, Institute of Biomaterials (WW 7), Cauerstraße 6, 91058 Erlangen, Germany
These authors contributed equally and share the first authorship for this article.
Search for more papers by this authorRainer Detsch
Friedrich-Alexander-University Erlangen-Nuremberg, Institute of Biomaterials (WW 7), Cauerstraße 6, 91058 Erlangen, Germany
These authors contributed equally and share the first authorship for this article.
Search for more papers by this authorStefan Romeis
Friedrich-Alexander-University Erlangen-Nuremberg, Institute of Particle Technology (LFG), Cauerstraße 4, 91058 Erlangen, Germany
These authors contributed equally and share the first authorship for this article.
Search for more papers by this authorJochen Schmidt
Friedrich-Alexander-University Erlangen-Nuremberg, Institute of Particle Technology (LFG), Cauerstraße 4, 91058 Erlangen, Germany
Search for more papers by this authorClaudia Eisermann
Friedrich-Alexander-University Erlangen-Nuremberg, Institute of Particle Technology (LFG), Cauerstraße 4, 91058 Erlangen, Germany
Search for more papers by this authorWolfgang Peukert
Friedrich-Alexander-University Erlangen-Nuremberg, Institute of Particle Technology (LFG), Cauerstraße 4, 91058 Erlangen, Germany
Search for more papers by this authorCorresponding Author
Aldo R. Boccaccini
Friedrich-Alexander-University Erlangen-Nuremberg, Institute of Biomaterials (WW 7), Cauerstraße 6, 91058 Erlangen, Germany
Correspondence to: A. R. Boccaccini (e-mail: [email protected])Search for more papers by this authorAbstract
In this study in vitro bioactivity and biocompatibility of two submicron 45S5 Bioglass® powders obtained by top-down processing have been evaluated and are compared to the as-received powder. Both submicron powders exhibited flake-like morphologies with lateral extensions of only a few microns; the flake thickness accounted for a few tens of nanometers. Enhanced in vitro bioactivity was found for the comminuted powders upon immersion in simulated body fluid. In vitro biocompatibility was evaluated by incubation of MG-63 osteoblast-like cells with various amounts (0–200 µg/mL) of the glass powders. Neither LDH-activity nor mitochondrial activity (WST-8) tests indicated cell toxicity. Increased mitochondrial activity was found for the submicron powders: incubation with high amounts revealed up to a threefold increase of osteoblast activity (ALP-activity). An overgrowth of the formed mineralized phase with phenotypical MG-63 cells was found by staining only for the submicron glasses. A distance ring is formed for the as-received powder. Superior bioactivity markers are found for shorter process times, that is, lower mass specific surface areas. This is attributed to the formation of carbonates during the comminution process. © 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 102B: 952–961, 2014.
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