Intraoral defect coverage with prelaminated epigastric fat flaps with human amniotic membrane in rats
Corresponding Author
Thomas Mücke
Department of Oral and Maxillofacial Surgery, Technische Universität München, Klinikum Rechts der Isar, University of Munich, Germany
Department of Oral and Maxillofacial Surgery, Technische Universität München, Klinikum Rechts der Isar, University of Munich, GermanySearch for more papers by this authorDenys J. Loeffelbein
Department of Oral and Maxillofacial Surgery, Technische Universität München, Klinikum Rechts der Isar, University of Munich, Germany
Search for more papers by this authorFrank Hölzle
Department of Oral and Maxillofacial Surgery, Technische Universität München, Klinikum Rechts der Isar, University of Munich, Germany
Search for more papers by this authorJulia Slotta-Huspenina
Institute of Pathology, Technische Universität München, Klinikum Rechts der Isar, University of Munich, Germany
Search for more papers by this authorAnna Borgmann
Department of Oral and Maxillofacial Surgery, Technische Universität München, Klinikum Rechts der Isar, University of Munich, Germany
Search for more papers by this authorAnastasios N. Kanatas
Oral and Facial Specialties Department, Pinderfields General Hospital, Wakefield, WF1 4DG, UK
Search for more papers by this authorDavid A. Mitchell
Oral and Facial Specialties Department, Pinderfields General Hospital, Wakefield, WF1 4DG, UK
Search for more papers by this authorStefan Wagenpfeil
Institute for Medical Statistics and Epidemiology, Technische Universität München, Klinikum Rechts der Isar, Germany
Search for more papers by this authorKlaus-Dietrich Wolff
Department of Oral and Maxillofacial Surgery, Technische Universität München, Klinikum Rechts der Isar, University of Munich, Germany
Search for more papers by this authorMarco R. Kesting
Department of Oral and Maxillofacial Surgery, Technische Universität München, Klinikum Rechts der Isar, University of Munich, Germany
Search for more papers by this authorCorresponding Author
Thomas Mücke
Department of Oral and Maxillofacial Surgery, Technische Universität München, Klinikum Rechts der Isar, University of Munich, Germany
Department of Oral and Maxillofacial Surgery, Technische Universität München, Klinikum Rechts der Isar, University of Munich, GermanySearch for more papers by this authorDenys J. Loeffelbein
Department of Oral and Maxillofacial Surgery, Technische Universität München, Klinikum Rechts der Isar, University of Munich, Germany
Search for more papers by this authorFrank Hölzle
Department of Oral and Maxillofacial Surgery, Technische Universität München, Klinikum Rechts der Isar, University of Munich, Germany
Search for more papers by this authorJulia Slotta-Huspenina
Institute of Pathology, Technische Universität München, Klinikum Rechts der Isar, University of Munich, Germany
Search for more papers by this authorAnna Borgmann
Department of Oral and Maxillofacial Surgery, Technische Universität München, Klinikum Rechts der Isar, University of Munich, Germany
Search for more papers by this authorAnastasios N. Kanatas
Oral and Facial Specialties Department, Pinderfields General Hospital, Wakefield, WF1 4DG, UK
Search for more papers by this authorDavid A. Mitchell
Oral and Facial Specialties Department, Pinderfields General Hospital, Wakefield, WF1 4DG, UK
Search for more papers by this authorStefan Wagenpfeil
Institute for Medical Statistics and Epidemiology, Technische Universität München, Klinikum Rechts der Isar, Germany
Search for more papers by this authorKlaus-Dietrich Wolff
Department of Oral and Maxillofacial Surgery, Technische Universität München, Klinikum Rechts der Isar, University of Munich, Germany
Search for more papers by this authorMarco R. Kesting
Department of Oral and Maxillofacial Surgery, Technische Universität München, Klinikum Rechts der Isar, University of Munich, Germany
Search for more papers by this authorAbstract
Background: The aim of this study was to investigate whether or not wound healing after the use of microvascular anastomosed fat flaps prelaminated with human amniotic membrane, for intraoral defect coverage, is improved when compared wth wound healing of pure fat flaps. Methods: Microsurgical transplantation of the superficial epigastric fat flap prelaminated with HAM was evaluated using 47 Sprague-Dawley rats. Standardized oral mucosa defects were created and covered by HAM or polyglactin910/polydioxanon patches only, prelaminated and bare flaps, uncovered or by HAM after flap insertion. After 7, 15, and 35 days, postoperatively, the flaps were reassessed. Results: The mean value of the defect size after 7 days was 47.73 ± 2.63 mm2 in the control, 48.63 ± 2.23 mm2 in the bare flaps covered by HAM after insertion, and 36.85 ± 2.79 mm2 in the prelaminated HAM group. The mean value of the wound closure time in all rats was 13.74 ± 2.05 days (range 11–18). Intraoral defects were covered with mucosa after 15.67 ± 1.66 days in the pure flap group and 11.89 ± 0.78 days in the HAM group (p < 0.0001). Conclusions: Prelaminated flaps with HAM used in the repair of large mucosa defects complete epithelialization from the surrounding margins faster than bare flaps. Wound healing can be enhanced by using HAM as a prelaminated epithelial structure within microvascular anastomosed flaps. © 2010 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2010.
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