Osteogenic differentiation of umbilical cord and adipose derived stem cells onto highly porous 45S5 Bioglass®-based scaffolds
Rainer Detsch
Institute of Biomaterials, Department of Materials Science and Engineering, University of Erlangen-Nuremberg, 91058 Erlangen Germany
Search for more papers by this authorSonja Alles
Institute for Technical Chemistry, Leibniz University, Hanover, Germany
Search for more papers by this authorJasmin Hum
Institute of Biomaterials, Department of Materials Science and Engineering, University of Erlangen-Nuremberg, 91058 Erlangen Germany
Search for more papers by this authorPeter Westenberger
FEI Visualization Sciences Group, Wernigeroder Str. 129, D-40595 Duesseldorf, Germany
Search for more papers by this authorFrank Sieker
GE Sensing & Inspection Technologies GmbH, Niels-Bohr-Straße 7, 31515 Wunstorf, Germany
Search for more papers by this authorDominik Heusinger
Institute of Biomaterials, Department of Materials Science and Engineering, University of Erlangen-Nuremberg, 91058 Erlangen Germany
Search for more papers by this authorCorresponding Author
Cornelia Kasper
Institute for Technical Chemistry, Leibniz University, Hanover, Germany
Department of Biotechnology, University of Natural Resources and Life Science, Vienna, Austria
Correspondence to: C. Kasper; e-mail: [email protected] or A. R. Boccaccini; e-mail: [email protected]Search for more papers by this authorCorresponding Author
Aldo R. Boccaccini
Institute of Biomaterials, Department of Materials Science and Engineering, University of Erlangen-Nuremberg, 91058 Erlangen Germany
Correspondence to: C. Kasper; e-mail: [email protected] or A. R. Boccaccini; e-mail: [email protected]Search for more papers by this authorRainer Detsch
Institute of Biomaterials, Department of Materials Science and Engineering, University of Erlangen-Nuremberg, 91058 Erlangen Germany
Search for more papers by this authorSonja Alles
Institute for Technical Chemistry, Leibniz University, Hanover, Germany
Search for more papers by this authorJasmin Hum
Institute of Biomaterials, Department of Materials Science and Engineering, University of Erlangen-Nuremberg, 91058 Erlangen Germany
Search for more papers by this authorPeter Westenberger
FEI Visualization Sciences Group, Wernigeroder Str. 129, D-40595 Duesseldorf, Germany
Search for more papers by this authorFrank Sieker
GE Sensing & Inspection Technologies GmbH, Niels-Bohr-Straße 7, 31515 Wunstorf, Germany
Search for more papers by this authorDominik Heusinger
Institute of Biomaterials, Department of Materials Science and Engineering, University of Erlangen-Nuremberg, 91058 Erlangen Germany
Search for more papers by this authorCorresponding Author
Cornelia Kasper
Institute for Technical Chemistry, Leibniz University, Hanover, Germany
Department of Biotechnology, University of Natural Resources and Life Science, Vienna, Austria
Correspondence to: C. Kasper; e-mail: [email protected] or A. R. Boccaccini; e-mail: [email protected]Search for more papers by this authorCorresponding Author
Aldo R. Boccaccini
Institute of Biomaterials, Department of Materials Science and Engineering, University of Erlangen-Nuremberg, 91058 Erlangen Germany
Correspondence to: C. Kasper; e-mail: [email protected] or A. R. Boccaccini; e-mail: [email protected]Search for more papers by this authorAbstract
In the context of bone tissue engineering (BTE), combinations of bioactive scaffolds with living cells are investigated to optimally yield functional bone tissue for implantation purposes. Bioactive glasses are a class of highly bioactive, inorganic materials with broad application potential in BTE strategies. The aim of this study was to evaluate bioactive glass (45S5 Bioglass®) samples of composition: 45 SiO2, 24.5 CaO, 24.5 Na2O, and 6 P2O5 (in wt%) as scaffold materials for mesenchymal stem cells (MSC). Pore architecture of the scaffolds as well as cell behavior in the three-dimensional environment was evaluated by several methods. Investigations concerned the osteogenic cell attachment, growth and differentiation of adipose tissue derived MSC (adMSC) compared with MSC from human full term umbilical cord tissues (ucMSC) on porous Bioglass®-based scaffolds over a cultivation period of 5 weeks. Differences in lineage-specific osteogenic differentiation of adMSC and ucMSC on Bioglass® samples were demonstrated. The investigation led to positive results in terms of cell attachment, proliferation, and differentiation of MSC onto Bioglass®-based scaffolds confirming the relevance of these matrices for BTE applications. © 2014 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 103A: 1029–1037, 2015.
Supporting Information
Additional Supporting Information may be found in the online version of this article.
| Filename | Description |
|---|---|
| jbma35238-sup-0001-suppFig1.tif199.5 KB |
Supplementary Figure 1 |
| jbma35238-sup-0001-suppFig2.tif213 KB |
Supplementary Figure 2 |
Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.
REFERENCES
- 1 Petite H, Viateau V, Bensaid W, Meunier A, de Pollak C, Bourguignon M, Oudina K, Sedel L, Guillemin G. Tissue-engineered bone regeneration. Nat Biotechnol 2000; 18: 959–963.
- 2 Schindeler A, McDonald MM, Bokko P, Little DG. Bone remodeling during fracture repair: The cellular picture. Semin Cell Dev Biol 2008; 19: 459–466.
- 3 Gerstenfeld LC, Cullinane DM, Barnes GL, Graves DT, Einhorn TA. Fracture healing as a post-natal developmental process: Molecular, spatial, and temporal aspects of its regulation. J Cell Biochem 2003; 88: 873–884.
- 4 Rezwan K, Chen QZ, Blaker JJ, Boccaccini AR. Biodegradable and bioactive porous polymer/inorganic composite scaffolds for bone tissue engineering. Biomaterials 2006; 27: 3413–3431.
- 5 Jones JR, Gentleman E, Polak J. Bioactive glass scaffolds for bone regeneration. Elements 2007; 3: 393–399.
- 6 Hutmacher DW. Scaffolds in tissue engineering bone and cartilage. Biomaterials 2000; 21: 2529–2543.
- 7
Detsch R,
Boccaccini AR. The role of osteoclasts in bone tissue engineering. J Tissue Eng Regen Med 2014. DOI: 10.1002/term.1851.
10.1002/term.1851 Google Scholar
- 8 Hench LL. The story of Bioglass®. J Mater Sci Mater Med 2006; 17: 967–978.
- 9 Xynos ID, Edgar AJ, Buttery LDK, Hench LL, Polak JM. Gene-expression profiling of human osteoblasts following treatment with the ionic products of Bioglass® 45S5 dissolution. J Biomed Mater Res 2001; 55: 151–157.
- 10 Oonishi H, Kushitani S, Yasukawa E, Iwaki H, Hench LL, Wilson J, Tsuji E, Sugihara T. Particulate bioglass compared with hydroxyapatite as a bone graft substitute. Clin Orthop Relat Res 1997: 316–325.
- 11 Gorustovich AA, Roether JA, Boccaccini AR. Effect of bioactive glasses on angiogenesis: A review of in vitro and in vivo evidences. Tissue Eng Part B Rev 2010; 16: 199–207.
- 12 Karageorgiou V, Kaplan D. Porosity of 3D biomaterial scaffolds and osteogenesis. Biomaterials 2005; 26: 5474–5491.
- 13 Zuk PA, Zhu M, Mizuno H, Huang J, Futrell JW, Katz AJ, Benhaim P, Lorenz HP, Hedrick MH. Multilineage cells from human adipose tissue: Implications for cell-based therapies. Tissue Eng 2001; 7: 211–228.
- 14 Bruder SP, Fink DJ, Caplan AI. Mesenchymal stem cells in bone development, bone repair, and skeletal regeneration therapy. J Cell Biochem 1994; 56: 283–294.
- 15
Jaiswal N,
Haynesworth SE,
Caplan AI,
Bruder SP. Osteogenic differentiation of purified, culture-expanded human mesenchymal stem cells in vitro. J Cell Biochem 1997; 64: 295–312.
10.1002/(SICI)1097-4644(199702)64:2<295::AID-JCB12>3.0.CO;2-I CAS PubMed Web of Science® Google Scholar
- 16 Wagner W, Wein F, Seckinger A, Frankhauser M, Wirkner U, Krause U, Blake J, Schwager C, Eckstein V, Ansorge W, Ho AD. Comparative characteristics of mesenchymal stem cells from human bone marrow, adipose tissue, and umbilical cord blood. Exp Hematol 2005; 33: 1402–1416.
- 17 Mauney JR, Volloch V, Kaplan DL. Role of adult mesenchymal stem cells in bone tissue-engineering applications: Current status and future prospects. Tissue Eng 2005; 11: 787–802.
- 18 Kim J, Shin JM, Jeon YJ, Chung HM, Chae JI. Proteomic validation of multifunctional molecules in mesenchymal stem cells derived from human bone marrow, umbilical cord blood and peripheral blood. PLoS One 2012; 7: e32350.
- 19 Chamberlain G, Fox J, Ashton B, Middleton J. Concise review: Mesenchymal stem cells: Their phenotype, differentiation capacity, immunological features, and potential for homing. Stem Cells 2007; 25: 2739–2749.
- 20 Dominici M, Le Blanc K, Mueller I, Slaper-Cortenbach I, Marini FC, Krause DS, Deans RJ, Keating A, Prockop DJ, Horwitz EM. Minimal criteria for defining multipotent mesenchymal stromal cells. The International Society for Cellular Therapy position statement. Cytotherapy 2006; 8: 315–317.
- 21 Schneider RK, Neuss S, Knüchel R, Perez-Bouza A. Mesenchymale Stammzellen für das „tissue engineering“des Knochens. Der Pathologe 2010; 31: 138–146.
- 22 Kern S, Eichler H, Stoeve J, Klüter H, Bieback K. Comparative analysis of mesenchymal stem cells from bone marrow, umbilical cord blood, or adipose tissue. Stem Cells 2006; 24: 1294–1301.
- 23 Chen QZ, Thompson ID, Boccaccini AR. 45S5 Bioglass®-derived glass–ceramic scaffolds for bone tissue engineering. Biomaterials 2006; 27: 2414–2425.
- 24 Wolbank S, Peterbauer A, Fahrner M, Hennerbichler S, van Griensven M, Stadler G, Redl H, Gabriel C. Dose-dependent immunomodulatory effect of human stem cells from amniotic membrane: A comparison with human mesenchymal stem cells from adipose tissue. Tissue Eng 2007; 13.
- 25 Broz A, Baresova V, Kromka A, Rezek B, Kalbacova M. Strong influence of hierarchically structured diamond nanotopography on adhesion of human osteoblasts and mesenchymal cells. physica status solidi (a) 2009; 206: 2038–2041.
- 26 Discher DE, Janmey P, Wang Y-l. Tissue cells feel and respond to the stiffness of their substrate. Science 2005; 310: 1139–1143.
- 27 Moby V, Kadi A, de Isla N, Stoltz JF, Menu P. Polyelectrolyte multilayer films: Effect of the initial anchoring layer on the cell growth. Bio-Med Mater Eng 2008; 18: 199–204.
- 28 Fabbri P, Valentini L, Hum J, Detsch R, Boccaccini AR. 45S5 Bioglass®-derived scaffolds coated with organic-inorganic hybrids containing graphene. Mater Sci Eng C Mater Biol Appl 2013; 33: 3592–3600.
- 29 Kress S, Neumann A, Weyand B, Kasper C. Stem cell differentiation depending on different surfaces. In: C Kasper, F Witte, R Pörtner, editors. Tissue Engineering III: Cell - Surface Interactions for Tissue Culture. Vol. 126. Berlin Heidelberg: Springer; 2012. p 263–28. Advances in Biochemical Engineering Biotechnology].
- 30 Aronin CEP, Sadik KW, Lay AL, Rion DB, Tholpady SS, Ogle RC, Botchwey EA. Comparative effects of scaffold pore size, pore volume, and total void volume on cranial bone healing patterns using microsphere-based scaffolds. J Biomed Mater Res A 2009; 89: 632–641.
- 31 Renghini C, Giuliani A, Mazzoni S, Brun F, Larsson E, Baino F, Vitale-Brovarone C. Microstructural characterization and in vitro bioactivity of porous glass-ceramic scaffolds for bone regeneration by synchrotron radiation X-ray microtomography. J Eur Ceram Soc 2013; 33: 1553–1565.
- 32 Jones JR, Poologasundarampillai G, Atwood RC, Bernard D, Lee PD. Non-destructive quantitative 3D analysis for the optimisation of tissue scaffolds. Biomaterials 2007; 28: 1404–1413.
- 33 Xynos ID, Hukkanen MV, Batten JJ, Buttery LD, Hench LL, Polak JM. Bioglass 45S5 stimulates osteoblast turnover and enhances bone formation In vitro: Implications and applications for bone tissue engineering. Calcif Tissue Int 2000; 67: 321–329.
- 34 Detsch R, Guillon O, Wondraczek L, Boccaccini AR. Initial attachment of rMSC and MG-63 cells on patterned bioglass ® substrates. Adv Eng Mater 2012; 14: B38–B44.
- 35 Roldan JC, Chang E, Kelantan M, Jazayeri L, Deisinger U, Detsch R, Reichert TE, Gurtner GC. Quantifying migration and polarization of murine mesenchymal stem cells on different bone substitutes by confocal laser scanning microscopy. J Cranio-Maxillofac Surg 2010; 38: 580–588.
- 36 Declercq HA, Gorski TL, Schacht EH, Cornelissen MJ. Osteoblast behaviour on in situ photopolymerizable three-dimensional scaffolds based on D,L-lactide and ε-caprolactone: Influence of pore volume, pore size and pore shape. J Mater Sci Mater Med 2008; 19: 3105–3114.
- 37 Kruyt MC, De Bruijn JD, Wilson CE, Oner FC, Van Blitterswijk CA, Verbout AJ, Dhert WJA. Viable osteogenic cells are obligatory for tissue-engineered ectopic bone formation in goats. Tissue Eng 2003; 9: 327–336.
- 38 Reilly GC, Radin S, Chen AT, Ducheyne P. Differential alkaline phosphatase responses of rat and human bone marrow derived mesenchymal stem cells to 45S5 bioactive glass. Biomaterials 2007; 28: 4091–4097.
- 39 Detsch R, Uhl F, Deisinger U, Ziegler G. 3D-Cultivation of bone marrow stromal cells on hydroxyapatite scaffolds fabricated by dispense-plotting and negative mould technique. J Mater Sci Mater Med 2008; 19: 1491–1496.
- 40 Silva AR, Paula AC, Martins TM, Goes AM, Pereria MM. Synergistic effect between bioactive glass foam and a perfusion bioreactor on osteogenic differentiation of human adipose stem cells. J Biomed Mater Res A 2014; 102: 818–827.
- 41 Ciavarella S, Dammacco F, De Matteo M, Loverro G, Silvestris F. Umbilical cord mesenchymal stem cells: Role of regulatory genes in their differentiation to osteoblasts. Stem Cells Dev 2009; 18: 1211–1220.
- 42 Thian ES, Huang J, Best SM, Barber ZH, Brooks RA, Rushton N, Bonfield W. The response of osteoblasts to nanocrystalline silicon-substituted hydroxyapatite thin films. Biomaterials 2006; 27: 2692–2698.
- 43 Lamers E, Frank Walboomers X, Domanski M, te Riet J, van Delft FCMJM, Luttge R, Winnubst LAJA, Gardeniers HJGE, Jansen JA. The influence of nanoscale grooved substrates on osteoblast behavior and extracellular matrix deposition. Biomaterials 2010; 31: 3307–3316.
- 44 Huang GTJ, Gronthos S, Shi S. Critical reviews in oral biology & medicine: Mesenchymal stem cells derived from dental tissues vs. those from other sources: Their biology and role in Regenerative Medicine. J Dent Res 2009; 88: 792–806.
- 45 Salgado AJ, Coutinho OP, Reis RL. Bone tissue engineering: State of the art and future trends. Macromol Biosci 2004; 4: 743–765.
Citing Literature
